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51.
52.
Zhang ZF Matsuda D Khoo SK Buzzitta K Block E Petillo D Richard S Anema J Furge KA Teh BT 《Mutation research》2008,657(1):77-83
Cytogenetic abnormalities, such as DNA amplifications and deletions, often lead to significant changes in gene expression levels within a chromosomal region. Instead of generating additional DNA copy number data, one method to identify DNA copy number abnormalities has been to search existing gene expression data for regional perturbations in gene expression. However, it is not clear how well this surrogate method performs in the examination of individual tumors and how we can use both DNA and RNA data to identify candidate genes that may be mutated. Here we report a comparison study using summarized DNA and RNA data to identify chromosomal abnormalities in human samples. Forty-four tissue samples from patients diagnosed as having renal cell carcinoma (RCC) were collected, together with 15 normal kidney samples as controls, and for each sample the genome-wide DNA and RNA data were obtained for comparison using Affymetrix 100K SNP and HGU133plus2 gene expression chips, respectively. The DNA and RNA data was summarized by both chromosome arm and cytogenetic banding patterns and compared. The result of this analysis revealed that the two summarized data sets used to identify cytogenetic changes agreed well. However, some differences between the two were also identified. These differences of large-scale gene expression deregulation without evidence of the comparable DNA copy number alterations may be the result of known mechanisms, such as large-scale methylation or chromosome inactivation, or may be the result of some new mechanism of DNA-RNA translation. The usefulness of the combined data set for identifying regions of mutated genes is also discussed. 相似文献
53.
54.
Lydia C. L. Teh Louise S. L. Teh Fung Chen Chung 《Biodiversity and Conservation》2008,17(13):3061-3077
Many marine protected areas (MPAs), particularly in developing countries, have failed because of a lack of enforcement and
monitoring due to limited public funds for conservation. Private investment and management in MPAs offers a potential solution,
and has been applied with initial positive results at the Sugud Islands Marine Conservation Area (SIMCA) in Sabah, Malaysia.
Conservation fees charged to visitors to Lankayan Island Dive Resort within the SIMCA have generated a sustainable source
of financing to meet the majority of management costs for the conservation area, which is separately managed by a private
organization called Reef Guardian. The availability of adequate funds has enabled Reef Guardian to invest in personnel training
and surveillance technology to enforce the rules and regulations of the conservation area. In collaboration with government
enforcement agencies, Reef Guardian has reduced threats such as illegal fishing and turtle egg poaching. As a result, there
is a comparatively high abundance of commercially important fish, and turtle nestings at Lankayan Island have increased. Private
management can be effective in conserving biodiversity in MPAs, and may well succeed regionally in suitable locations.
相似文献
Lydia C. L. TehEmail: |
55.
56.
Pichler H Baumgartner S Freudenschuss M Palme S Binder EM Krska R 《Mycotoxin Research》2001,17(2):202-205
Enzyme-linked immunosorbent assays (ELISA) proved to be a fast and simple method for the detection of mycotoxins and other undesired contaminants in food and feed. The present study is focused on the optimisation and exploitation of the egg yolk antibody technology in order to develop competitive ELISAs for the detection of mycotoxins in cereals. Due to its importance as one of the most relevant Fusarium mycotoxins, the trichothecene deoxynivalenol (DON) was selected as representative. Chickens were immunised with different protein conjugates performing varying booster intervals. The antibodies were isolated by the poly(ethylene glycol) precipitation method according to Polson. By use of these antibodies an indirect competitive ELISA was developed for the detection of DON. First investigations of naturally contaminated wheat samples showed a good correspondence with results obtained by GC-ECD when calibration in blank wheat extracts was performed. 相似文献
57.
J. H. D. Bassett A. A. J. Pannett S. A. Forbes R. V. Thakker M. McCarthy A. P. Read B. T. Teh C. Larsson S. Kytölä J. Leisti P. Salmela G. Weber S. Giraud C. X. Zhang A. Calender J. W. M. Höppener H. K. Ploos van Amstel C. J. M. Lips K. Kas W. J. M. Van de Ven P. Gaudray 《Human genetics》1997,100(5-6):657-665
Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder characterised by tumours of the parathyroids,
pancreas and anterior pituitary. The MEN1 gene has been localised to a 2-Mb region of chromosome 11q13 by meiotic mapping
studies in MEN1 families. Such studies may have a limited resolution of approximately 1 cM (i.e. 1 Mb) and we have therefore
investigated 96 MEN1 families (40 British, 17 French, 12 Finnish, 7 Swedish, 7 Dutch, 7 North American, 2 Australian, 1 New
Zealand, 1 German, 1 Spanish and 1 Danish) for linkage disequilibrium, in order to facilitate a finer mapping resolution.
We have utilised five microsatellite DNA sequence polymorphisms from the candidate region and have accurately determined their
allele sizes, which ranged from 161 bp to 272 bp. The heterozygosity and number of alleles (given in brackets), respectively,
at the loci were: D11S1883 (76%, 11), D11S457 (55%, 5), PYGM (94%, 18), D11S1783 (10%, 4) and D11S449 (87%, 16). Allelic association
was assessed by Chi-square 2 ×n contingency tables, by Fisher exact 2 ×n contingency tables and by a likelihood-based approach. The results of haplotype analysis revealed 91 different affected haplotypes
in the 96 families, an identical affected haplotype being observed in no more than two families. These results indicate the
absence of an ancestral affected haplotype. Significant linkage disequilibrium (P < 0.005) could be established amongst the microsatellite loci but not between the loci and MEN1 in either the total population
or in any of the geographical sub-populations. The absence of linkage disequilibrium between MEN1 and the polymorphic loci
is probably the result of the occurrence of multiple different disease-causing mutations in MEN1.
Received: 1 April 1997 / Accepted: 25 June 1997 相似文献
58.
N. J. Lassam Zheng Lin Michael G. Shennan Anouk Courseaux Bin T. Teh Patrick Gaudray Catharina Larsson 《Human genetics》1997,99(6):776-780
MLK-3 kinase is a widely expressed serine/ threonine kinase that bears multiple protein interaction domains and regulates
signals mediated by the stress-responsive pathway. Thus, MLK-3 signaling affects numerous cellular processes, raising the
possibility that MLK-3 might play a role in oncogenesis. In this report, we describe the fine mapping of the MLK-3 gene within the 11q13.1 chromosomal region. By integrating data from somatic cell hybrids and double color fluorescence in
situ hybridization on metaphase chromosomes and DNA fibers, MLK-3 has been assigned approximately 1 Mb telomeric of PYGM, close to the D11S546 locus. Since the MEN1 susceptibility locus is also located within the 11q13.1 region, we have carried
out Southern and Northern blot analyses, as well as protein truncation assays to establish whether abnormalities in MLK-3 lead to the development of this familial cancer syndrome. Our observations exclude MLK-3 as the MEN1 gene.
Received: 25 September 1996 / Revised: 16 December 1996 相似文献
59.
Julie M. Koeman Ryan C. Russell Min-Han Tan David Petillo Michael Westphal Katherine Koelzer Julie L. Metcalf Zhongfa Zhang Daisuke Matsuda Karl J. Dykema Heather L. Houseman Eric J. Kort Laura L. Furge Richard J. Kahnoski Stphane Richard Annick Vieillefond Pamela J. Swiatek Bin Tean Teh Michael Ohh Kyle A. Furge 《PLoS genetics》2008,4(9)
60.
Chien Teh Chen Cheng Chang Li Ching Huei Kao 《Journal of Plant Growth Regulation》1991,10(1-4):201-205
Leaf senescence of a chlorophylldeficient rice mutant (LT-8) was investigated. At 10 days after planting, the chlorophyll level in the third leaves of rice seedlings of the mutant was about one half that of normal leaves (Norin no. 8), whereas no difference in the protein level could be detected in the two genotypes. The protein level in leaves decreased with increasing age, and no significant difference could be detected during senescence in the two genotypes. Chlorophyll level in the normal leaves also decreased with increasing age. However, the chlorophyll level in the mutant leaves began to decrease only after more than 60% of the initial protein had been degraded. The pattern of ethylene production in the normal leaves was, in general, similar to that in the mutant leaves. Ethylene production first decreased with age, increased to a maximum at day 18, and decreased thereafter. Both spermidine and spermine levels in the leaves of the two genotypes decreased with increasing age. The pattern of the putrescine level in the normal leaves behaved somewhat similar to that in the mutant leaves. However, during the course of senescence, the putrescine level in the mutant leaves was always higher than that in the normal leaves. The possible relationship between endogenous polyamine levels and ethylene production is discussed. 相似文献