全文获取类型
收费全文 | 311篇 |
免费 | 14篇 |
出版年
2013年 | 4篇 |
2012年 | 4篇 |
2011年 | 5篇 |
2009年 | 9篇 |
2008年 | 3篇 |
2007年 | 7篇 |
2006年 | 7篇 |
2005年 | 3篇 |
2004年 | 6篇 |
2000年 | 3篇 |
1999年 | 9篇 |
1998年 | 11篇 |
1997年 | 13篇 |
1996年 | 6篇 |
1995年 | 6篇 |
1994年 | 7篇 |
1993年 | 7篇 |
1992年 | 8篇 |
1991年 | 8篇 |
1990年 | 10篇 |
1989年 | 12篇 |
1988年 | 17篇 |
1987年 | 13篇 |
1986年 | 10篇 |
1985年 | 5篇 |
1984年 | 5篇 |
1982年 | 6篇 |
1981年 | 9篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1976年 | 4篇 |
1975年 | 4篇 |
1973年 | 4篇 |
1972年 | 3篇 |
1971年 | 4篇 |
1966年 | 3篇 |
1959年 | 3篇 |
1958年 | 7篇 |
1957年 | 6篇 |
1956年 | 6篇 |
1955年 | 3篇 |
1954年 | 5篇 |
1953年 | 5篇 |
1952年 | 7篇 |
1951年 | 4篇 |
1950年 | 2篇 |
1948年 | 2篇 |
1946年 | 2篇 |
排序方式: 共有325条查询结果,搜索用时 15 毫秒
51.
Several apiaceous and two asteraceous species were tested for their suitability to support larval development of the carrot fly. Plants grown in pots or transplanted from seed beds into pots, were inoculated with a specific number of eggs. Pupae and non-pupated larvae were collected 6–7 wk after inoculation. Both the number and weights of pupae produced varied widely among the species. Cultivated carrots Daucus carota sativus often gave rise to only moderate numbers of pupae, but these invariably attained the highest weights. Pimpinella major was the only apiaceous plant tested that did not yield any carrot flies. The two asteraceous plants Cichorium intybus and Tanacetum vulgare failed to support larval development. Total carrot fly biomass produced per plant was influenced by both the host species and the root weight. Emergence rates of adult flies were positively correlated with pupal weights. Small individuals tended to have a longer total developmental time from egg to adult fly. 相似文献
52.
Resistance to Brevicoryne brassicae has been identified in the progeny of two selected kale (B. oleracea var. acephala) plants, one from the F1 hybrid cultivar ‘Arsis RS’ and one from the landrace ‘Butzo’. These plants were crossed with susceptible B. oleracea morphotypes that have different periods to flowering. The type of susceptible plant line used had an effect on the resistance phenotypc of the progeny. Tested F2 populations derived from these crosses show that resistance is not under simple genetic control. This, in addition to variation in aphid numbers within accessions, suggests that separation of genetic components of control from environmental ‘noise’ for any accession may only be possible by the production of double haploid plant lines. 相似文献
53.
54.
Drosophila melanogaster are found in sympatry with Drosophila simulans, and matings between the species produce nonfertile hybrid offspring at low frequency. Evolutionary theory predicts that females choose mates, so males should alter their behaviour in response to female cues. We show that D. melanogaster males quickly decrease courtship towards D. simulans females. Courtship levels are reduced within 5 min of exposure to a heterospecific female, and overall courtship is significantly lower than courtship towards conspecific females. To understand changes at the molecular level during mate choice, we performed microarray analysis on D. melanogaster males that courted heterospecific D. simulans females and found nine genes have altered expression compared with controls. In contrast, males that court conspecific females alter expression of at least 35 loci. The changes elicited by conspecific courtship likely modulate nervous system function to reinforce positive conspecific signals and dampen the response to heterospecific signals. 相似文献
55.
David J Lee Lewis EH Bingle Karin Heurlier Mark J Pallen Charles W Penn Stephen JW Busby Jon L Hobman 《BMC microbiology》2009,9(1):252
Background
Homologous recombination mediated by the λ-Red genes is a common method for making chromosomal modifications in Escherichia coli. Several protocols have been developed that differ in the mechanisms by which DNA, carrying regions homologous to the chromosome, are delivered into the cell. A common technique is to electroporate linear DNA fragments into cells. Alternatively, DNA fragments are generated in vivo by digestion of a donor plasmid with a nuclease that does not cleave the host genome. In both cases the λ-Red gene products recombine homologous regions carried on the linear DNA fragments with the chromosome. We have successfully used both techniques to generate chromosomal mutations in E. coli K-12 strains. However, we have had limited success with these λ-Red based recombination techniques in pathogenic E. coli strains, which has led us to develop an enhanced protocol for recombineering in such strains. 相似文献56.
57.
Cabbage root fly ( Delia brassicae ) egg-laying around radish plants ranging in age from newly emerged seedlings to plants with flowering shoots was studied in the field and laboratory. The attractiveness of radish changed with age, one peak in egg-laying occurring just before plants were of marketable size and further peaks as they produced flowering shoots. The pattern of egg-laying was similar for the six radish cultivars tested, both in the field and laboratory, irrespective of whether flies did or did not have a choice of plants of different ages. At any one age, there was no correlation between egg-laying and plant size. Selection within radish cultivars for the extremes of the range of egg-laying preference appeared to alter the cycle of attractiveness of different radish stocks to cabbage root fly, the response being influenced by environmental conditions. 相似文献
58.
59.
P. R. ELLIS B. D. DOWKER G. H. FREEMAN J. A. HARDMAN 《The Annals of applied biology》1985,106(2):349-356
In field trials between 1973 and 1982 families of carrots of cv. Long Chantenay were assessed for their resistance to carrot fly (Psila rosae). In some but not all years, families selected for their observed resistance in earlier trials seemed more resistant than the parent line. In more than half the trials there was an association between carrot fly damage and root density, there being less damage on those families from which more roots were harvested. Thus, family selection within cv. Long Chantenay did not lead to a consistent improvement in resistance to carrot fly. Alternative strategies for achieving plant resistance to this pest are discussed. 相似文献
60.
MILLICENT L. FREAN F.L.S. ELLIS MARKS 《Botanical journal of the Linnean Society. Linnean Society of London》1988,97(3):255-259
FREAN, M. L. & MARKS, E., 1988. Chromosome numbers of C3 and C4 variants within the species Alloteropais semialata (R.Br.) Hitch. (Poaceae). In a study of mid-lamina leaf sections, different variants of A. semialata were found to have C3 or C4 anatomy. The C4 leaf showed a specialized photosynthetic vascular bundle sheath not present in the C3 form. Chromosome counts made from pollen mother cell squashes showed that the C3 form of A. semialata is a fertile diploid 2n = 2x = 18 and the C4 form, a fertile allohexaploid 2n = 6x = 54. The cytological evidence suggests that the two forms should be considered as separate species. 相似文献