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81.
KATRIN FRIEDEMANN GRIT KUNERT ELENA GORB STANISLAV N. GORB ROLF G. BEUTEL 《Ecological Entomology》2015,40(6):732-740
1. Sympatric populations of insects adapted to different host plants are good model systems not only to study how they adapt to the chemistry of their food plant, but also to investigate whether morphological modifications evolved enabling them to live successfully on a certain plant species. 2. The pea aphid, Acyrthosiphon pisum (Harris) encompasses at least 11 genetically distinct sympatric host races, each showing a preference for a certain legume species. The leaflet surfaces of these legumes differ considerably in their wax coverage. 3. It was investigated whether the attachment structures of three pea aphid genotypes from different host races are adapted to the different surface properties of their host plants and whether they show differences in their attachment ability on the respective host and non‐host plants. 4. The surface morphology of plants and aphid tarsi was examined using SEM (scanning electron microscopy). The ability of the aphids to walk on specific surfaces was tested using traction force measurements. 5. The presence of wax blooms on the leaflets lowers the aphids' attachment ability considerably and diminishes their subsequent attachment on ‘neutral’ surfaces like glass. The pea aphid host races differ in their ability to walk on certain surfaces. However, the genotype from the adapted aphid host race was not necessarily the one with the best walking performance on their host plant. All aphids, regardless of the original host plant, were most efficient on the neutral control surface glass. The general host plant Vicia faba was the plant with the most favourable surface for all aphid host races. 相似文献
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83.
ABSTRACT Spectrographic analysis showed that dholes produce sounds with two fundamental frequencies (components): the high-frequency and the low-frequency, which may occur simultaneously or separately. The fundamental frequency of the low-frequency component varied from 0.52 to 1.44 kHz, and that of the high-frequency component from 5.51 to 10.77 kHz. In calls where both the frequencies occurred simultaneously, they were not integer multiples of each other. They also had different frequency modulations and had additional combinative frequency bands. These are features of biphonation. Our data showed biphonation occurs in a lot of dhole calls (20 to 92% of the calls, depending on the individual (n=14); average occurrence 44.3%, n=1317 sounds). The occurrence of biphonation did not differ between sexes and ages; however, occurrence of the high component only was significantly higher in subadult animals, whereas the occurrence of the low component only was significantly higher in adults. Based on the sound structures, we discuss probable mechanisms of sound production for both the components in the dhole. For the low component, the normal vocal folds oscillation mechanism is suggested. For the high component, four possible mechanisms of sound production are discussed. We conclude that the vortex-shedding mechanism is the most probable. 相似文献
84.
ILYA VOLODIN MARTIN KAISER VERA MATROSOVA ELENA VOLODINA ANNA KLENOVA OLGA FILATOVA 《Bioacoustics.》2013,22(3):277-278
ABSTRACT Here we present an acoustic approach for reliable sexing in four whistling duck species from the genus Dendrocygna and compare it with molecular and cloacal inspection techniques. In the four examined species, the White-faced Whistling Duck D. viduata, Fulvous Whistling Duck D. bicolor, Cuban Whistling Duck D. arborea and Red-billed Whistling Duck D. autumnalis, sexes are indistinguishable by appearance. However all the four species show strong sexual differences in the structure of their species-specific loud whistles. For 59 examined birds, an acoustic-based sexing showed 100% accordance to the DNA PCR analysis, while the cloacal inspection showed only 89.8% accuracy. The results demonstrate that acoustic sexing represents a feasible alternative to the two traditional methods as a noninvasive tool for the distant sexing of the four whistling duck species both in captivity and in the wild. 相似文献
85.
86.
RONALD C KESSLER MATTHIAS ANGERMEYER JAMES C ANTHONY RON DE GRAAF KOEN DEMYTTENAERE ISABELLE GASQUET GIOVANNI DE GIROLAMO SEMYON GLUZMAN OYE GUREJE JOSEP MARIA HARO NORITO KAWAKAMI AIMEE KARAM DAPHNA LEVINSON MARIA ELENA MEDINA MORA MARK A OAKLEY BROWNE JOSé POSADA-VILLA DAN J STEIN CHEUK HIM ADLEY TSANG SERGIO AGUILAR-GAXIOLA JORDI ALONSO SING LEE STEVEN HEERINGA BETH-ELLEN PENNELL PATRICIA BERGLUND MICHAEL J GRUBER MARIA PETUKHOVA SOMNATH CHATTERJI T. BEDIRHAN üSTüN 《World psychiatry》2007,6(3):168-176
Data are presented on the lifetime prevalence, projected lifetime risk,
and age-of-onset distributions of mental disorders in the World Health Organization
(WHO)''s World Mental Health (WMH) Surveys. Face-to-face community surveys
were conducted in seventeen countries in Africa, Asia, the Americas, Europe,
and the Middle East. The combined numbers of respondents were 85,052. Lifetime
prevalence, projected lifetime risk, and age of onset of DSM-IV disorders
were assessed with the WHO Composite International Diagnostic Interview (CIDI),
a fully-structured lay administered diagnostic interview. Survival analysis
was used to estimate lifetime risk. Median and inter-quartile range (IQR)
of age of onset is very early for some anxiety disorders (7-14, IQR: 8-11)
and impulse control disorders (7-15, IQR: 11-12). The age-of-onset distribution
is later for mood disorders (29-43, IQR: 35-40), other anxiety disorders (24-50,
IQR: 31-41), and substance use disorders (18-29, IQR: 21-26). Median and IQR
lifetime prevalence estimates are: anxiety disorders 4.8-31.0% (IQR: 9.9-16.7%),
mood disorders 3.3-21.4% (IQR: 9.8-15.8%), impulse control disorders 0.3-25.0%
(IQR: 3.1-5.7%), substance use disorders 1.3-15.0% (IQR: 4.8-9.6%), and any
disorder 12.0-47.4% (IQR: 18.1-36.1%). Projected lifetime risk is proportionally
between 17% and 69% higher than estimated lifetime prevalence (IQR: 28-44%),
with the highest ratios in countries exposed to sectarian violence (Israel,
Nigeria, and South Africa), and a general tendency for projected risk to be
highest in recent cohorts in all countries. These results document clearly
that mental disorders are commonly occurring. As many mental disorders begin
in childhood or adolescents, interventions aimed at early detection and treatment
might help reduce the persistence or severity of primary disorders and prevent
the subsequent onset of secondary disorders. 相似文献
87.
ALBERTO SANTINI VITTORIO LUCCHINI ELENA FABBRI ETTORE RANDI 《Molecular ecology resources》2007,7(6):955-961
Individual genotypes determined from noninvasive DNA samples (typically extracted from shed hairs or scats) are used to estimate population size in monitoring projects of elusive species. However, polymerase chain reaction (PCR) success rates usually are lower, and genotyping errors higher than in standard population genetic surveys, due to DNA degradation or contamination in aged field samples. In this study, we evaluate the results of common garden experiments showing that DNA degradation is significant in wolf (Canis lupus) scats older than 3 days, and it is enhanced in scats in direct contact with soil. A storage test showed that samples kept frozen in 95% ethanol performed better compared to other methods. However, variance of PCR success among samples was high, independent on sample age or storage condition. The detrimental consequences of DNA degradation can be avoided by collecting scat samples as fresh as possible, and implementing efficient multitube procedures and stringent quality control of the laboratory results. Efficient multitube procedures can produce reliable data, like in this study, which showed that the consensus genotypes obtained from excremental DNA exactly matched distinct reference genotypes obtained from wolf blood samples. 相似文献
88.
CHIARA ATZORI FIORENZA AGOSTONI ELENA ANGELI GIOVANNA ORLANDO ANTONIETTA CARGNEL 《The Journal of eukaryotic microbiology》1996,43(5):42S-42S
SUMMARY Pneumocystis carinii pneumonia (PCP) is usually diagnosed by examination of BAL, a sample often unpleasant to be collected from immunocompromised host affected by acute respiratory disease. We studied by the Internal Transcribed Spacers (ITSs) nested PCR the presence of P. carinii DNA in serum and Peripheral Blood Mononuclear Cells (PBMC) during acute episodes of PCP to test blood as a possible noninvasive diagnostic tool. 相似文献
89.
90.
ELENA VALSECCHI DEBBIE GLOCKNER-FERRARI MARK FERRARI & WILLIAM AMOS 《Molecular ecology》1998,7(10):1419-1422
Sloughed whale skin contains enough DNA for genetic analysis, and offers a nonintrusive method for collecting tissue. Here, we examine the efficiency of sloughed skin sampling using 1460 samples collected from free-ranging humpback whales. Samples were sexed and screened for up to 10 microsatellite markers. The vast majority of samples appear genetically compatible with field observations. About 1% of groups revealed more genotypes than whales, but we argue that this is more likely to be due to unobserved whales than to contamination. Sloughed skin sampling is particularly effective when applied to active groups and offers a viable alternative to biopsy darting in regions where darting is either not permitted or otherwise undesirable. 相似文献