全文获取类型
收费全文 | 757284篇 |
免费 | 84751篇 |
国内免费 | 329篇 |
出版年
2018年 | 6673篇 |
2016年 | 9013篇 |
2015年 | 11951篇 |
2014年 | 14084篇 |
2013年 | 20303篇 |
2012年 | 22727篇 |
2011年 | 23251篇 |
2010年 | 15810篇 |
2009年 | 14741篇 |
2008年 | 21220篇 |
2007年 | 21790篇 |
2006年 | 20698篇 |
2005年 | 19705篇 |
2004年 | 19630篇 |
2003年 | 18741篇 |
2002年 | 18336篇 |
2001年 | 32007篇 |
2000年 | 32106篇 |
1999年 | 26135篇 |
1998年 | 9485篇 |
1997年 | 9907篇 |
1996年 | 9429篇 |
1995年 | 8674篇 |
1994年 | 8673篇 |
1993年 | 8625篇 |
1992年 | 21960篇 |
1991年 | 21428篇 |
1990年 | 21145篇 |
1989年 | 20820篇 |
1988年 | 19475篇 |
1987年 | 18624篇 |
1986年 | 17301篇 |
1985年 | 17301篇 |
1984年 | 14295篇 |
1983年 | 12525篇 |
1982年 | 9631篇 |
1981年 | 8585篇 |
1980年 | 8297篇 |
1979年 | 13883篇 |
1978年 | 10885篇 |
1977年 | 9837篇 |
1976年 | 9518篇 |
1975年 | 10286篇 |
1974年 | 11212篇 |
1973年 | 11003篇 |
1972年 | 10146篇 |
1971年 | 9136篇 |
1970年 | 8048篇 |
1969年 | 7741篇 |
1968年 | 7227篇 |
排序方式: 共有10000条查询结果,搜索用时 187 毫秒
951.
952.
The ergosterol and lecithin absorption IR-spectra were studied in a nonpolar anhydrous medium. The thermodynamical and spectral characteristics of dimeric associates and the enthalpy value for trimeric associates of this sterol are determined. Thermodynamical and spectral parameters of ergosterol intermolecular associates with lecithin in a nonpolar anhydrous medium are found. It is established that the intermolecular interaction of lecithin with ergosterol occurs according to the mechanism of hydrogen bond. A conclusion is drawn that the presence of binary bonds and methyl groups in the cyclic and aliphatic parts of the sterol molecule affects greatly the structure of the model membrane and its strength. It is shown that under conditions of the experiment the oxygen of the phosphate group contributes to formation of the molecular associates of lecithin with sterols and not that of the carbonyl group. The obtained experimental data may be at use when studying structural disturbances of native membranes in norm and with different pathologies. 相似文献
953.
954.
E I Pimenova 《Ukrainski? biokhimicheski? zhurnal》1989,61(5):88-92
Tryptophan deaminase was isolated from Proteus vulgaris and purified. The procedure for enzyme purification included the cell destruction on USD-1, fractionation by ammonium sulphate, gel chromatography on ultragel AcA34, ion exchange chromatography on DEAE-cellulose. A degree of the enzyme purification--95, yield--5.7%. The pH optimum was 7.5, the temperature optimum--47 degrees C. The enzyme molecular weight (105 kD) was estimated by gel chromatography on Sephadex G-200, Km--5.0 mM in the K-phosphate buffer (pH 7.5). The SH groups are supposed to be present in the active site of the enzyme. The enzyme does not accelerate oxidation deamination of phenylalanine and tyrosine. 相似文献
955.
956.
957.
B Hollenbach E Scherzinger K Schweiger R Lurz H Lehrach E E Wanker 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》1999,354(1386):991-994
We have shown previously by electron microscopy that the purified glutathione S-transferase (GST)-Huntington's disease (HD) exon 1 fusion protein with 51 glutamine residues (GST-HD51) is an oligomer, and that site-specific proteolytic cleavage of this fusion protein results in the formation of insoluble more highly ordered protein aggregates with a fibrillar or ribbon-like morphology (E. Scherzinger et al. (1997) Cell 90, 549-558). Here we report that a truncated GST HD exon 1 fusion protein with 51 glutamine residues, which lacks the proline-rich region C-terminal to the polyglutamine (polyQ) tract (GST-HD51 delta P) self-aggregates into high-molecular-mass protein aggregates without prior proteolytic cleavage. Electron micrographs of these protein aggregates revealed thread-like fibrils with a uniform diameter of ca. 25 nm. In contrast, proteolytic cleavage of GST-HD51 delta P resulted in the formation of numerous clusters of high-molecular-mass fibrils with a different, ribbon-like morphology. These structures were reminiscent of prion rods and beta-amyloid fibrils in Alzheimer's disease. In agreement with our previous results with full-length GST-HD exon 1, the truncated fusion proteins GST-HD20 delta P and GST-HD30 delta P did not show any tendency to form more highly ordered structures, either with or without protease treatment. 相似文献
958.
959.
960.