全文获取类型
收费全文 | 1089621篇 |
免费 | 119501篇 |
国内免费 | 701篇 |
专业分类
1209823篇 |
出版年
2018年 | 10093篇 |
2016年 | 13734篇 |
2015年 | 18666篇 |
2014年 | 21930篇 |
2013年 | 30995篇 |
2012年 | 34861篇 |
2011年 | 35380篇 |
2010年 | 24265篇 |
2009年 | 22287篇 |
2008年 | 31626篇 |
2007年 | 32678篇 |
2006年 | 30735篇 |
2005年 | 29664篇 |
2004年 | 29487篇 |
2003年 | 28160篇 |
2002年 | 27496篇 |
2001年 | 47530篇 |
2000年 | 47788篇 |
1999年 | 38163篇 |
1998年 | 13882篇 |
1997年 | 14443篇 |
1996年 | 13543篇 |
1995年 | 12664篇 |
1994年 | 12331篇 |
1993年 | 12234篇 |
1992年 | 31534篇 |
1991年 | 30831篇 |
1990年 | 30225篇 |
1989年 | 29393篇 |
1988年 | 27606篇 |
1987年 | 25986篇 |
1986年 | 24284篇 |
1985年 | 24043篇 |
1984年 | 20097篇 |
1983年 | 17067篇 |
1982年 | 13147篇 |
1981年 | 11756篇 |
1980年 | 11155篇 |
1979年 | 18697篇 |
1978年 | 14626篇 |
1977年 | 13338篇 |
1976年 | 12371篇 |
1975年 | 13597篇 |
1974年 | 14733篇 |
1973年 | 14651篇 |
1972年 | 13292篇 |
1971年 | 12080篇 |
1970年 | 10568篇 |
1969年 | 10273篇 |
1968年 | 9582篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
Wendy A. Douglass Robert H. Hyland Christopher D. Buckley Aymen Al-Shamkhani Jacqueline M. Shaw Sarah L. Scarth David L. Simmons S.K.Alex Law 《FEBS letters》1998,440(3):125
The cysteine-rich region (CRR) of the β2 integrin subunit was replaced by that of β1 to give the chimera β2NV1. β2NV1 can combine with αL to form a variant leukocyte-function-associated antigen (LFA)-1 on COS cell surface, suggesting that the specificity of the β2 interaction with αL does not lie in the CRR. Unlike those expressing wild-type LFA-1, COS cells expressing αLβ2NV1 are constitutively active in intercellular adhesion molecule (ICAM)-1 adhesion. These results suggest that activation of LFA-1 involves the release of an intramolecular constraint, which is maintained, in part, by the authentic β2 CRR. 相似文献
102.
103.
An algorithm is proposed that allows one to identify the MHD mode structure in toroidal plasmas by processing signals from Mirnov probes measuring plasma MHD activity. The algorithm differs fundamentally from the diagnostic methods presently used in tokamaks, being simpler and more efficient. The algorithm is based on constructing an analytic signal using the Hilbert transformation of the Mirnov signals at a given instant. The phase and amplitude dependences obtained take into account the toroidal effects and allow one to determine the number and amplitude of the excited MHD mode. The algorithm was approbated with both test signals and actual signals from MHD diagnostics in the T-10 tokamak. It is demonstrated that the algorithm can be used to analyze single-mode MHD instabilities in toroidal plasmas. 相似文献
104.
105.
D S Faffe G H Silva P M Kurtz E M Negri V L Capelozzi P R Rocco W A Zin 《Journal of applied physiology》2001,90(4):1400-1406
The dynamic mechanical properties of lung tissue and its contents of collagen and elastic fibers were studied in strips prepared from mice instilled intratracheally with saline (C) or silica [15 (S15) and 30 days (S30) after instillation]. Resistance, elastance, and hysteresivity were studied during oscillations at different frequencies on S15 and S30. Elastance increased from C to silica groups but was similar between S15 and S30. Resistance was augmented from C to S15 and S30 and was greater in S30 than in S15 at higher frequencies. Hysteresivity was higher in S30 than in C and S15. Silica groups presented a greater amount of collagen than did C. Elastic fiber content increased progressively along time. This increment was related to the higher amount of oxytalan fibers at 15 and 30 days, whereas elaunin and fully developed elastic fibers were augmented only at 30 days. Silicosis led not only to pulmonary fibrosis but also to fibroelastosis, thus assigning a major role to the elastic system in the silicotic lung. 相似文献
106.
Disseminated nocardiosis in three macaque monkeys 总被引:1,自引:0,他引:1
Extrapulmonary nocardiosis was diagnosed at necropsy in two rhesus monkeys (Macaca mulatta) and one pigtailed monkey (M. nemestrina) over a four-year period in a large primate center. Typical lesions were multiple pyogranulomatous foci in the liver, intestines, peritoneum, lung and brain. Partially acid-fast, branching, filamentous organisms were seen in all lesions. Nocardia sp. was isolated from two cases. We postulate that two of the monkeys were infected by the oral route because of the distribution of lesions. 相似文献
107.
Mild chymotrypsin digestion of native lipase (449 amino acids) preferentially cleaved the Phe 335-Ala 336 bond. On SDS-gel electrophoresis, 3 major bands were observed: band 1 (52 kDa) representing native lipase, bands 2 and 3 (40 and 12 kDa) representing the two lipase fragments A and B. Fragment A does not retain lipase activity but maintains its ability to adsorb to interfaces. Fragment B was identified with the lipase C-terminal region (336-449). It does not exhibit any activity towards tributyrylglycerol emulsions and any ability to adsorb to interfaces. 相似文献
108.
109.
The marine bacteriumVibrio anguillarum causes disease in fish worldwide and is particularly devastating in aquaculture. Little is known about the ecology ofV. anguillarum in the environment and how this may relate to the pathogenicity of this organism. Combining membrane filtration and a species-specific DNA probe, culturableV. anguillarum cells were detected in water from three habitats and in chinook salmon (Onchorynchus tshawytscha) tissue samples. Results show that different marine habitats have a marked effect on cell numbers and that water temperature may play a role in the culturability and distribution ofV. anguillarum. Vibrio anguillarum was detected from the gills of salmon within 24 h of transfer of fingerlings from freshwater to seawater, with cell numbers reaching a concentration of 1.9 × 102 cells g–1 tissue 28 days post transfer.Vibrio anguillarum cell numbers were low in the colon throughout the study, andV. anguillarum was not detected in healthy kidney samples. The methodology reported in this paper allows the accurate quantification of culturableV. anguillarum cells and has allowed a preliminary study of the ecology of this species. 相似文献
110.
The fish pathogenVibrio anguillarum causes significant economic losses in commercially cultured fish species worldwide. At present, identification ofV. anguillarum requires conventional isolation and culturing techniques. Using differential hybridization, a 310 base pairV. anguillarum-specific DNA fragment was isolated for use as a probe. In specificity studies against 19 different bacterial species, including twoVibrio sp. and fish pathogens, and 223 marine bacterial isolates, the probe hybridized exclusively toV. anguillarum strains. The probe also strongly hybridizes to 7 of 9 serotypes tested, with serotype 09 giving a weak probe reaction and serotype O7 negative. The probe allows rapid and accurate detection of both pathogenic and environmental strains ofV. anguillarum. 相似文献