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71.
Khrapova NP Lesovoĭ VS Prokhvatilova EV Stupenko LN Zharkova SF 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1999,(6):70-74
In this work conditions for the reproduction of hybridoma technology, specially adapted to C. neoformans, for obtaining monoclonal hybridomas (McAb) to diagnostically significant antigens of C. neoformans, the infective agent of cryptococcosis, are presented. The advantages of using the short-time cycle of stimulation of mouse B lymphocytes with low doses of C. neoformans capsular polysaccharide and the effectiveness of the hybridization of mouse spleen cells with myeloma cells, line Sp2/0, are shown. Four lines of stable hybridomas, producing McAb to different epitopes of C. neoformans surface antigens, have been obtained. The specific activity of McAb has been studied in the indirect immunofluorescence assay, the cytochemical and solid-phase enzyme immunoassays (EIA). McAb 3E2, Cr2 and 2G9 have been shown to be suitable for use in diagnostic EIA systems. 相似文献
72.
Ganina KP Borodaĭ NV Petunin IuI Kliushin DA Pilipenko VN 《T?Sitologii?a i genetika》1999,33(6):45-52
The estimation of the malignancy-associated changes in oral mucosa of patients with benign and malignant processes in mammary and thyroid gland is carried out on the basis of textural and densitometric indices of the interphase nuclei of the epitheliocytes with the help of the methods of mathematical statistics. 相似文献
73.
Deragacheva OIu Meĭers IE Burikov AA 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2004,90(9):1094-1102
The nucleus pontis oralis' neurons were responsive to electrical stimulation of posterior hypothalamus. PS-on neurons showed an inhibitory response, and PS-off cells demonstrated an excitatory response. Neurons that discharged in association with phasic paradoxical sleep phenomena were found to have both the excitatory and the inhibitory responses. Evoked responses changed across sleep-waking cycle. The findings suggest that posterior hypothalamus is involved in the control of paradoxical sleep generation mechanisms located in the nucleus pontis oralis. 相似文献
74.
Zvezdochkina NV Muranova LN Andrianov VV Arkhipova SS Gaĭnutdinov KhL Golubev AI Pleshchinskiĭ IN 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2004,90(11):1381-1392
Two groups of rats with different level of motor activities: high- and low-active animals, were distinguished. The blockade of dopamine receptors by haloperidol led to depression of locomotor activity in both groups of rats; in grape snails, haloperidol caused a decrease of the velocity of locomotor responses. In was found that within 5 minutes of intravenous injection of haloperidol the excitability of spinal centers of rats decreased; but in 30 minutes in started restoring. Chronic application of the preparation depressed the effect of posttetanic potentiation of H-response in gastrocnemius muscle of spinal rats. In command neurons of grape snail, chronic injections of haloperidol causes a significant hyperpolarization shift of membrane potential and an increase of threshold of the generation of action potential. It was shown that the selective pharmacological inhibition of dopaminergic system of the brain led to a decrease of excitability in some determined neurons of the snail and spinal motor centers of rats, as well as inhibited the locomotor responses both in vertebrate and in invertebrate animals. 相似文献
75.
Dvoretskiĭ DP Timoshenko TE Belobokova NK 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2004,90(11):1356-1362
Changes in microcirculation during low-intensity He-Ne laser irradiation were studied by measurement of blood vessels and lymphangions' diameter in anesthetized rat mesentery using method of videomicroscopy in vivo. We demonstrated that the red coherent light induced a significant increase in contractility of vessels' smooth muscle cells. The flow decreased in the mesenteric microvessels during H2O2-mediated oxidant stress was restored after He-Ne laser irradiation. 相似文献
76.
77.
Grigor'eva TA Aĭzman RI Shoshenko KA 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2004,90(11):1370-1380
The study of mitotic activity of mesenteric microvascular endothelium cells (EC) in 4-, 12-, and 30-day old rats has been carried out using following parameters: number of labeled nuclei per vessel, or per 100 microm of vascular length, or per 1 mm2 of endothelial surface area, as well as shares of labeled EC and of vessels with labeled EC, have been estimated. The highest density of labeled nuclei was revealed in the pericapillary vessels in all rats. Its values were significantly higher in 12-day-old rats and were the lowest in 30-day-old ones. 相似文献
78.
79.
Pirutin SK Turovetskiĭ VB Pirutina OV Kudriashov IuB 《Radiatsionnaia biologiia, radioecologiia / Rossi?skaia akademiia nauk》2004,44(4):442-444
It was detected that exposure of macrophages to red light (600-740 nm) led to the changes in their intracellular pH and hydrolytic activity. The character of these changes depends on the initial level of pH in the cells. The maximum effect of irradiation is detected if the initial pH level is low. It is possible that Na/H-exchanger takes part in normalizing effect of red light on intracellular pH level. 相似文献
80.
Pirutin SK Turovetskiĭ VB Pirutina OV Kudriashov IuB 《Radiatsionnaia biologiia, radioecologiia / Rossi?skaia akademiia nauk》2004,44(4):438-441
It was shown that UV-irradiation caused damage to mice peritoneal macrophage plasma membranes. A decrease in extracellular Ca2+ leads to a decrease of the damaging effect. An increase in extracellular Ca2+ or adding of calcium ionophore A23187 to the medium is accompanied by an increase in a number of damaged cells. These data allow us to suppose that modification of the damaging effect of UV-irradiation by Ca2+ ions can be bound with changing of electric stability of membrane lipid matrix. 相似文献