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101.
Characterisation of Rhizobium isolates by amplification of DNA polymorphisms using random primers. 总被引:6,自引:0,他引:6
S P Harrison L R Mytton L Sk?t M Dye A Cresswell 《Canadian journal of microbiology》1992,38(10):1009-1015
The use of single random primers, selected in the absence of target sequence information, has been shown to be effective in producing DNA amplifications that provide fingerprints which are unique to individual organisms. DNA amplification by random priming was applied to the DNA from isolates of Rhizobium leguminosarum biovar trifolii. Amplification products were produced using a number of primers, and the resulting fingerprints allowed strain differentiation. However, the effectiveness of primers was dependent upon length and GC content. It was also possible to amplify DNA directly from cells in culture and in nodule tissue. Lysis of these cells was achieved simply through heat applied in the initial DNA denaturation stage of the thermal reaction. The ability to produce varied amplification patterns from different Rhizobium isolates, especially directly from nodules, gives this method potential for use in examining genetic structures and relationships in Rhizobium populations. 相似文献
102.
A note on some factors affecting the survival of Rhizobium cultures during freeze drying and subsequent storage 总被引:3,自引:2,他引:1
M. Dye 《Journal of applied microbiology》1982,52(3):461-464
Viable counts of Rhizobium before and immediately after freeze drying in sucrose-peptone medium (SPM) showed that neither culture age nor cell concentration affected survival. SPM gave greater protection during drying than either dextran-sucrose-glutamate medium or distilled water. The half-lives of freeze-dried cultures stored at 4°C were estimated using an accelerated storage test and were dependent on both the strain and the suspending medium used. It is recommended that Rhizobium cultures, prepared by the procedures used in the Rothamsted Collection of Rhizobium , should be redried at intervals of 30 years. 相似文献
103.
Thomas S. Dye 《PloS one》2014,9(7)
This study illustrates how the theory of directed graphs can be used to investigate the structure and growth of the leeward Kohala field system, a traditional Hawaiian archaeological site that presents an unparalleled opportunity to investigate relative chronology. The relative chronological relationships of agricultural walls and trails in two detailed study areas are represented as directed graphs and then investigated using graph theoretic concepts including cycle, level, and connectedness. The structural properties of the directed graphs reveal structure in the field system at several spatial scales. A process of deduction yields a history of construction in each detailed study area that is different than the history produced by an earlier investigation. These results indicate that it is now possible to study the structure and growth of the entire field system remnant using computer software implementations of graph theoretic concepts applied to observations of agricultural wall and trail intersections made on aerial imagery and/or during fieldwork. A relative chronology of field system development with a resolution of one generation is a possible result. 相似文献
104.
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106.
This article describes the relationship between 10 selected properties of the sediments (chlorophyll a and b, colloidal and total carbohydrate, water concentration, sediment type, organic matter, erosion threshold and erosion rate)
and meio- and macrofauna within and among three different habitats in an urbanized intertidal mudflat/mangrove forest in Tambourine
Bay, Sydney Harbour, Australia. Many of the biogeochemical variables were significantly different among habitats, often grading
from mudflat to mangrove canopy. In contrast to previous studies, patterns of distribution of macrofauna among habitats were
weak. For the meiofauna, only copepods showed any significant difference among habitats, with the greatest numbers in the
open mudflat habitat and least under the mangrove canopy. There was a gradient in fauna among the habitats; overall macrofauna
abundances were greatest under the mangrove canopy and least on the mudflat, while meiofauna abundance was greatest in the
pneumatophore habitat and least under the canopy. Correlations between fauna and properties of sediment were generally weak.
When the habitats were analysed separately, some correlations were strengthened but relationships were inconsistent. Thus,
while some taxa vary significantly among habitats there was not a strong relationship between biogeochemical properties and
either macro- or meiofauna. This suggests that localised factors other than the measured properties of the sediments are driving
patterns in fauna at these small scales, which requires further investigation to be unravelled. 相似文献
107.
Background
Identification of novel drug targets and their inhibitors is a major challenge in the field of drug designing and development. Diaminopimelic acid (DAP) pathway is a unique lysine biosynthetic pathway present in bacteria, however absent in mammals. This pathway is vital for bacteria due to its critical role in cell wall biosynthesis. One of the essential enzymes of this pathway is dihydrodipicolinate synthase (DHDPS), considered to be crucial for the bacterial survival. In view of its importance, the development and prediction of potent inhibitors against DHDPS may be valuable to design effective drugs against bacteria, in general. 相似文献108.
The development and impact of tuberculosis vaccines 总被引:9,自引:0,他引:9
Vaccination is expected to make a major contribution to the goal of eliminating tuberculosis worldwide by 2050. But developing a new effective vaccine will require innovation in scientific research, a proactive approach to clinical trials of new vaccine candidates, and application of vaccines as part of an integrated approach to disease control. 相似文献
109.
Cyclin D1 regulates cellular migration through the inhibition of thrombospondin 1 and ROCK signaling
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Li Z Wang C Jiao X Lu Y Fu M Quong AA Dye C Yang J Dai M Ju X Zhang X Li A Burbelo P Stanley ER Pestell RG 《Molecular and cellular biology》2006,26(11):4240-4256
Cyclin D1 is overexpressed in human tumors, correlating with cellular metastasis, and is induced by activating Rho GTPases. Herein, cyclin D1-deficient mouse embryo fibroblasts (MEFs) exhibited increased adhesion and decreased motility compared with wild-type MEFs. Retroviral transduction of cyclin D1 reversed these phenotypes. Mutational analysis of cyclin D1 demonstrated that its effects on cellular adhesion and migration were independent of the pRb and p160 coactivator binding domains. Genomewide expression arrays identified a subset of genes regulated by cyclin D1, including Rho-activated kinase II (ROCKII) and thrombospondin 1 (TSP-1). cyclin D1(-/-) cells showed increased Rho GTP and ROCKII activity and signaling, with increased phosphorylation of LIM kinase, cofilin (Ser3), and myosin light chain 2 (Thr18/Ser19). Cyclin D1 repressed ROCKII and TSP-1 expression, and the migratory defect of cyclin D1(-/-) cells was reversed by ROCK inhibition or TSP-1 immunoneutralizing antibodies. cyclin E knockin to the cyclin D1(-/-) MEFs rescued the DNA synthesis defect of cyclin D1(-/-) MEFs but did not rescue either the migration defect or the abundance of ROCKII. Cyclin D1 promotes cellular motility through inhibiting ROCK signaling and repressing the metastasis suppressor TSP-1. 相似文献
110.