Binding of multivalent ligands to mobile receptors in membranes

We present a model to describe the equilibrium binding properties for the attachment of multivalent ligands to mobile receptors in membranes. The interaction is assumed to be governed by two inherently different association constants. The first of these controls the initial attachment of a ligand to its first receptor, by adsorption from bulk solution, while the second governs subsequent receptor attachments to this initially bound ligand by rearrangement of membrane-bound species. Simple statistical mechanical expressions are used to estimate contributions to these association constants that are attributable to losses of translational and rotational degrees of freedom occurring upon binding. Suitable combinatorial expressions are combined with these association constants to derive the concentrations of bound species and the binding isotherms. Examination of these expressions leads to the conclusion that once initially bound, most multivalent ligands will be completely saturated by receptors and that partially bound species will be essentially nonexistent. This behavior is attributable to the generally high overall affinities of these ligands and to the mobility of the membrane-bound species. Some specific comments are made, in light of this theory, about the binding of cholera toxin to its membrane receptor, the ganglioside G_M1.     

Responses to a Drought in the Interior Lowlands of Papua New Guinea: A Comparison of Bedamuni and Kubo-Konai

The ways that people experience, respond to and pattern recovery from major climatic aberrations must be understood within the context of existing socioeconomic arrangements and the ethos that informs these. This paper describes immediate and longer term impacts of a major drought on two populations—Bedamuni and Kubo-Konai—in the interior lowlands of Papua New Guinea. Though they occupy similar environments, are culturally related and reliant on similar technology and resources, these two populations differ in density, intensity of land use, and social complexity. The drought of 1997 affected one of the populations much more severely than the other. A comparison of effects on subsistence regimes, mobility and social life in the two areas suggests that these were mediated by understandings people held of relationships with both the environment and other people. Bedamuni pattern their lives around an expectation of favorable returns on effort, emphasising security of tenure to protect those returns. Kubo-Konai, in contrast, pattern their lives around an expectation that availability of resources will be often in flux, and emphasise means of ensuring security of supply. These understandings are reflected, respectively, in risk-prone and risk-averse strategies of subsistence and sociality which directly influence vulnerability and responses to disruptive events.     

Neural coding in antennal olfactory cells of tsetse flies (Glossina spp.)

Spike trains from individual antennal olfactory cells of tsetse flies (Glossina spp.) obtained during steady-state conditions (spontaneous as well as during stimulation with 1-octen-3-ol) and dynamic stimulation with repetitive pulses of 1-octen-3-ol were investigated by studying the spike frequency and the temporal structure of the trains. In general, stimulation changes the intensity of the spike activity but leaves the underlying stochastic structure unaffected. This structure turns out to be a renewal process. The only independently varying parameter in this process is the mean interspike interval length, suggesting that olfactory cells of tsetse flies may transmit information via a frequency coding. In spike records with high firing rates, however, the stationary records had significant negative first- order serial correlation coefficients and were non-renewal. Some cells in this study were capable of precisely encoding the onset of the odour pulses at frequencies up to at least 3 Hz. Cells with a rapid return to pre-stimulus activity at the end of stimulation responded more adequately to pulsed stimuli than cells with a long increased spike frequency. While short-firing cells process information via a frequency code, long-firing cells responded with two distinctive phases: a phasic, non-renewal response and a tonic, renewal response which may function as a memory of previous stimulations.      

Diet and urinary excretion of lignans in female subjects

Lignans, a class of compounds having a 2,3-dibenzylbutane skeleton, have recently been identified for the first time in humans and animals and evidence indicating their formation by intestinal microflora has previously been established in rats and humans. In the present report the influence of diet on the biosynthesis of this new group of compounds was investigated by comparing the urinary excretion of the principal lignan, trans-2,3-bis-(3-hydroxybenzyl) -butyrolactone (enterolactone, HBBL), in 12 omnivoric and 14 vegetarian women. Young vegetarian women were found to excrete significantly greater amounts of enterolactone than omnivores, while old vegetarians excreted comparable amounts to the omnivore group. A statistically significant (P less than 0.01-0.001) correlation was found between the amount of fibre in the diet and the urinary enterolactone excretion.     

Microbiological Quality Control of Sterilized Products: Evaluation of a Model Relating Frequency of Contaminated Items with Increasing Radiation Treatment

A model describing the dependence of the proportion of items contaminated ( P ) in a population of items on radiation dose ( D ) has been developed. The model was evaluated for a range of doses assuming a Poissonian distribution of micro-organisms on items prior to and during irradiation and radiation inactivation according to 'multiple-hit' kinetics; curves relating P and D were constructed. The influence on curve shape of changing the initial average number of micro-organisms on items and of varying parameters of microbial sensitivity to radiation was assessed. Consideration was given to the value of using sub-process radiation doses in microbiological quality control testing.     

An electron microscope study of sunflower crown gall tumor

Summary The inception and developmental phases of sunflower (Helianthus annuus) crown gall tumor growth induced byAgrobacterium tumefaciens were studied submicroscopically. Bacteria were found only in the inception phase. Chloroplasts from tumor cells of both phases showed several kinds of structural variation. Other cytoplasmic organelles remained more or less normal.This investigation was supported in part by an American Cancer Society Institutional Grant.