全文获取类型
收费全文 | 2626篇 |
免费 | 306篇 |
国内免费 | 1篇 |
专业分类
2933篇 |
出版年
2022年 | 27篇 |
2021年 | 52篇 |
2020年 | 25篇 |
2019年 | 44篇 |
2018年 | 38篇 |
2017年 | 34篇 |
2016年 | 64篇 |
2015年 | 120篇 |
2014年 | 111篇 |
2013年 | 140篇 |
2012年 | 164篇 |
2011年 | 173篇 |
2010年 | 85篇 |
2009年 | 86篇 |
2008年 | 162篇 |
2007年 | 124篇 |
2006年 | 97篇 |
2005年 | 116篇 |
2004年 | 106篇 |
2003年 | 80篇 |
2002年 | 74篇 |
2001年 | 43篇 |
2000年 | 56篇 |
1999年 | 60篇 |
1998年 | 31篇 |
1997年 | 24篇 |
1996年 | 27篇 |
1995年 | 18篇 |
1994年 | 25篇 |
1993年 | 15篇 |
1992年 | 50篇 |
1991年 | 38篇 |
1990年 | 43篇 |
1989年 | 41篇 |
1988年 | 36篇 |
1987年 | 38篇 |
1986年 | 22篇 |
1985年 | 30篇 |
1984年 | 20篇 |
1983年 | 15篇 |
1982年 | 14篇 |
1980年 | 19篇 |
1979年 | 28篇 |
1978年 | 23篇 |
1977年 | 22篇 |
1975年 | 15篇 |
1974年 | 23篇 |
1972年 | 15篇 |
1970年 | 13篇 |
1967年 | 15篇 |
排序方式: 共有2933条查询结果,搜索用时 15 毫秒
991.
Naturalists and scientists have been captivated by the diversity of marine larval forms since they were discovered following the advent of the microscope. Because they often bear little resemblance to adults, larvae were identified initially as new life forms, classified into different groups based on the similarity of their body plans and given new names that are still with us today. The radically different body plans and lifestyles of marine larvae and adults have led most investigators historically to study the two phases of complex life cycles in isolation. More recently, important ecological insights have sprung from taking a holistic view of marine life cycles. Meanwhile, the evolutionary (phenotypic and genetic) links among life-history phases remain less appreciated. In this review, our objective is to evaluate the evolutionary links within marine life cycles, and explore their ecological and evolutionary consequences. We provide a brief overview of marine life histories, discuss the phenotypic and genetic links between the two phases of the life cycle and pose challenges to advance our understanding of the evolutionary constraints acting on marine life histories. 相似文献
992.
Environmental uncertainty and the global biogeography of cooperative breeding in birds 总被引:1,自引:0,他引:1
Understanding why organisms as different as amoebas, ants, and birds cooperate remains an important question in evolutionary biology. Although ecology can influence cooperation and conflict within animal societies and has been implicated in species differences in sociality, the environmental predictors of sociality across broad geographic and taxonomic scales remain poorly understood. In particular, the importance of temporal variation in selection pressure has been underestimated in most evolutionary studies. Environmental uncertainty resulting from climatic variation is likely to be an important driver of temporal variation in selection pressure and therefore is expected to impact the evolution of behavioral, morphological, and physiological traits, including cooperation. Using a data set of over 95% of the world's birds, we examine the global geography and environmental, biotic, and historical biogeographic predictors of avian social behavior. We find dramatic spatial variation in social behavior for which environmental and biotic factors--namely, among-year environmental variability in precipitation--are important predictors. Although the clear global biogeographic structure in avian social behavior carries a strong signal of evolutionary history, environmental uncertainty plays an additional key role in explaining the incidence and distribution of avian cooperative breeding behavior. 相似文献
993.
The mshA::Tn5 mutant of Mycobacterium smegmatis does not produce mycothiol (MSH) and was found to markedly overproduce both ergothioneine and an ~15-kDa protein determined to be organic hydroperoxide resistance protein (Ohr). An mshA(G32D) mutant lacking MSH overproduced ergothioneine but not Ohr. Comparison of the mutant phenotypes with those of the wild-type strain indicated the following: Ohr protects against organic hydroperoxide toxicity, whereas ergothioneine does not; an additional MSH-dependent organic hydroperoxide peroxidase exists; and elevated isoniazid resistance in the mutant is associated with both Ohr and the absence of MSH. Purified Ohr showed high activity with linoleic acid hydroperoxide, indicating lipid hydroperoxides as the likely physiologic targets. The reduction of oxidized Ohr by NADH was shown to be catalyzed by lipoamide dehydrogenase and either lipoamide or DlaT (SucB). Since free lipoamide and lipoic acid levels were shown to be undetectable in M. smegmatis, the bound lipoyl residues of DlaT are the likely source of the physiological dithiol reductant for Ohr. The pattern of occurrence of homologs of Ohr among bacteria suggests that the ohr gene has been distributed by lateral transfer. The finding of multiple Ohr homologs with various sequence identities in some bacterial genomes indicates that there may be multiple physiologic targets for Ohr proteins. 相似文献
994.
Hands S Sajjad MU Newton MJ Wyttenbach A 《The Journal of biological chemistry》2011,286(52):44512-44520
Neurodegenerative diseases are characterized by intra- and/or extracellular protein aggregation and oxidative stress. Intense attention has been paid to whether protein aggregation itself contributes to abnormal production of free radicals and ensuing cellular oxidative damage. Although this question has been investigated in the context of extracellular protein aggregation, it remains unclear whether protein aggregation inside cells alters the redox homeostasis. To address this, we have used in vitro and in vivo (cellular) models of Huntington disease, one of nine polyglutamine (poly(Q)) disorders, and examined the causal relationship among intracellular protein aggregation, reactive oxygen species (ROS) production, and toxicity. Live imaging of cells expressing a fragment of huntingtin (httExon1) with a poly(Q) expansion shows increased ROS production preceding cell death. ROS production is poly(Q) length-dependent and not due to the httExon 1 flanking sequence. Aggregation inhibition by the MW7 intrabody and Pgl-135 treatment abolishes ROS production, showing that increased ROS is caused by poly(Q) aggregation itself. To examine this hypothesis further, we determined whether aggregation of poly(Q) peptides in vitro generated free radicals. Monitoring poly(Q) protein aggregation using atomic force microscopy and hydrogen peroxide (H(2)O(2)) production over time in parallel we show that oligomerization of httEx1Q53 results in early generation of H(2)O(2). Inhibition of poly(Q) oligomerization by the single chain antibody MW7 abrogates H(2)O(2) formation. These results demonstrate that intracellular protein aggregation directly causes free radical production, and targeting potentially toxic poly(Q) oligomers may constitute a therapeutic target to counteract oxidative stress in poly(Q) diseases. 相似文献
995.
Qu Y Misaghi S Newton K Gilmour LL Louie S Cupp JE Dubyak GR Hackos D Dixit VM 《Journal of immunology (Baltimore, Md. : 1950)》2011,186(11):6553-6561
Apoptotic cell death is important for embryonic development, immune cell homeostasis, and pathogen elimination. Innate immune cells also undergo a very rapid form of cell death termed pyroptosis after activating the protease caspase-1. The hemichannel pannexin-1 has been implicated in both processes. In this study, we describe the characterization of pannexin-1-deficient mice. LPS-primed bone marrow-derived macrophages lacking pannexin-1 activated caspase-1 and secreted its substrates IL-1β and IL-18 normally after stimulation with ATP, nigericin, alum, silica, flagellin, or cytoplasmic DNA, indicating that pannexin-1 is dispensable for assembly of caspase-1-activating inflammasome complexes. Instead, thymocytes lacking pannexin-1, but not the P2X7R purinergic receptor, were defective in their uptake of the nucleic acid dye YO-PRO-1 during early apoptosis. Cell death was not delayed but, unlike their wild-type counterparts, Panx1(-/-) thymocytes failed to recruit wild-type peritoneal macrophages in a Transwell migration assay. These data are consistent with pannexin-1 liberating ATP and other yet to be defined "find me" signals necessary for macrophage recruitment to apoptotic cells. 相似文献
996.
The immortal and totipotent properties of the germ line depend on determinants within the germ plasm. A common characteristic of germ plasm across phyla is the presence of germ granules, including P granules in Caenorhabditis elegans, which are typically associated with the nuclear periphery. In C. elegans, nuclear pore complex (NPC)-like FG repeat domains are found in the VASA-related P-granule proteins GLH-1, GLH-2, and GLH-4 and other P-granule components. We demonstrate that P granules, like NPCs, are held together by weak hydrophobic interactions and establish a size-exclusion barrier. Our analysis of intestine-expressed proteins revealed that GLH-1 and its FG domain are not sufficient to form granules, but require factors like PGL-1 to nucleate the localized concentration of GLH proteins. GLH-1 is necessary but not sufficient for the perinuclear location of granules in the intestine. Our results suggest that P granules extend the NPC environment in the germ line and provide insights into the roles of the PGL and GLH family proteins. 相似文献
997.
Rattanavong S Wuthiekanun V Langla S Amornchai P Sirisouk J Phetsouvanh R Moore CE Peacock SJ Buisson Y Newton PN 《Applied and environmental microbiology》2011,77(2):532-536
Melioidosis is a major cause of morbidity and mortality in Southeast Asia, where the causative organism (Burkholderia pseudomallei) is present in the soil. In the Lao People's Democratic Republic (Laos), B. pseudomallei is a significant cause of sepsis around the capital, Vientiane, and has been isolated in soil near the city, adjacent to the Mekong River. We explored whether B. pseudomallei occurs in Lao soil distant from the Mekong River, drawing three axes across northwest, northeast, and southern Laos to create nine sampling areas in six provinces. Within each sampling area, a random rice field site containing a grid of 100 sampling points each 5 m apart was selected. Soil was obtained from a depth of 30 cm and cultured for B. pseudomallei. Four of nine sites (44%) were positive for B. pseudomallei, including all three sites in Saravane Province, southern Laos. The highest isolation frequency was in east Saravane, where 94% of soil samples were B. pseudomallei positive with a geometric mean concentration of 464 CFU/g soil (95% confidence interval, 372 to 579 CFU/g soil; range, 25 to 10,850 CFU/g soil). At one site in northwest Laos (Luangnamtha), only one sample (1%) was positive for B. pseudomallei, at a concentration of 80 CFU/g soil. Therefore, B. pseudomallei occurs in Lao soils beyond the immediate vicinity of the Mekong River, alerting physicians to the likelihood of melioidosis in these areas. Further studies are needed to investigate potential climatic, soil, and biological determinants of this heterogeneity. 相似文献
998.
Newton RJ Vandewalle JL Borchardt MA Gorelick MH McLellan SL 《Applied and environmental microbiology》2011,77(19):6972-6981
The complexity of fecal microbial communities and overlap among human and other animal sources have made it difficult to identify source-specific fecal indicator bacteria. However, the advent of next-generation sequencing technologies now provides increased sequencing power to resolve microbial community composition within and among environments. These data can be mined for information on source-specific phylotypes and/or assemblages of phylotypes (i.e., microbial signatures). We report the development of a new genetic marker for human fecal contamination identified through microbial pyrotag sequence analysis of the V6 region of the 16S rRNA gene. Sequence analysis of 37 sewage samples and comparison with database sequences revealed a human-associated phylotype within the Lachnospiraceae family, which was closely related to the genus Blautia. This phylotype, termed Lachno2, was on average the second most abundant fecal bacterial phylotype in sewage influent samples from Milwaukee, WI. We developed a quantitative PCR (qPCR) assay for Lachno2 and used it along with the qPCR-based assays for human Bacteroidales (based on the HF183 genetic marker), total Bacteroidales spp., and enterococci and the conventional Escherichia coli and enterococci plate count assays to examine the prevalence of fecal and human fecal pollution in Milwaukee's harbor. Both the conventional fecal indicators and the human-associated indicators revealed chronic fecal pollution in the harbor, with significant increases following heavy rain events and combined sewer overflows. The two human-associated genetic marker abundances were tightly correlated in the harbor, a strong indication they target the same source (i.e., human sewage). Human adenoviruses were routinely detected under all conditions in the harbor, and the probability of their occurrence increased by 154% for every 10-fold increase in the human indicator concentration. Both Lachno2 and human Bacteroidales increased specificity to detect sewage compared to general indicators, and the relationship to a human pathogen group suggests that the use of these alternative indicators will improve assessments for human health risks in urban waters. 相似文献
999.
Some lichens have a super-hydrophobic upper surface, which repels water drops, keeping the surface dry but probably preventing
water uptake. Spore ejection requires water and is most efficient just after rainfall. This study was carried out to investigate
how super-hydrophobic lichens manage water uptake and repellence at their fruiting bodies, or podetia. Drops of water were
placed onto separate podetia of Cladonia chlorophaea and observed using optical microscopy and cryo-scanning-electron microscopy (cryo-SEM) techniques to determine the structure
of podetia and to visualise their interaction with water droplets. SEM and optical microscopy studies revealed that the surface
of the podetia was constructed in a three-level structural hierarchy. By cryo-SEM of water-glycerol droplets placed on the
upper part of the podetium, pinning of the droplet to specific, hydrophilic spots (pycnidia/apothecia) was observed. The results
suggest a mechanism for water uptake, which is highly sophisticated, using surface wettability to generate a passive response
to different types of precipitation in a manner similar to the Namib Desert beetle. This mechanism is likely to be found in
other organisms as it offers passive but selective water control. 相似文献
1000.
Crassulacean acid metabolism (CAM) and the capacity to store large quantities of water are thought to confer high water use
efficiency (WUE) and survival of succulent plants in warm desert environments. Yet the highly variable precipitation, temperature
and humidity conditions in these environments likely have unique impacts on underlying processes regulating photosynthetic
gas exchange and WUE, limiting our ability to predict growth and survival responses of desert CAM plants to climate change.
We monitored net CO2 assimilation (A
net), stomatal conductance (g
s), and transpiration (E) rates periodically over 2 years in a natural population of the giant columnar cactus Carnegiea gigantea (saguaro) near Tucson, Arizona USA to investigate environmental and physiological controls over carbon gain and water loss
in this ecologically important plant. We hypothesized that seasonal changes in daily integrated water use efficiency (WUEday) in this constitutive CAM species would be driven largely by stomatal regulation of nighttime transpiration and CO2 uptake responding to shifts in nighttime air temperature and humidity. The lowest WUEday occurred during time periods with extreme high and low air vapor pressure deficit (D
a). The diurnal with the highest D
a had low WUEday due to minimal net carbon gain across the 24 h period. Low WUEday was also observed under conditions of low D
a; however, it was due to significant transpiration losses. Gas exchange measurements on potted saguaro plants exposed to experimental
changes in D
a confirmed the relationship between D
a and g
s. Our results suggest that climatic changes involving shifts in air temperature and humidity will have large impacts on the
water and carbon economy of the giant saguaro and potentially other succulent CAM plants of warm desert environments. 相似文献