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排序方式: 共有719条查询结果,搜索用时 578 毫秒
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Kerstin Brinkmann Paul Waring Stefan P Glaser Verena Wimmer Denny L Cottle Ming Shen Tham Duong Nhu Lachlan Whitehead Alex RD Delbridge Guillaume Lessene Ian M Smyth Marco J Herold Gemma L Kelly Stephanie Grabow Andreas Strasser 《The EMBO journal》2020,39(24)
Studies of gene‐targeted mice identified the roles of the different pro‐survival BCL‐2 proteins during embryogenesis. However, little is known about the role(s) of these proteins in adults in response to cytotoxic stresses, such as treatment with anti‐cancer agents. We investigated the role of BCL‐XL in adult mice using a strategy where prior bone marrow transplantation allowed for loss of BCL‐XL exclusively in non‐hematopoietic tissues to prevent anemia caused by BCL‐XL deficiency in erythroid cells. Unexpectedly, the combination of total body γ‐irradiation (TBI) and genetic loss of Bcl‐x caused secondary anemia resulting from chronic renal failure due to apoptosis of renal tubular epithelium with secondary obstructive nephropathy. These findings identify a critical protective role of BCL‐XL in the adult kidney and inform on the use of BCL‐XL inhibitors in combination with DNA damage‐inducing drugs for cancer therapy. Encouragingly, the combination of DNA damage‐inducing anti‐cancer therapy plus a BCL‐XL inhibitor could be tolerated in mice, at least when applied sequentially. 相似文献
713.
Joshua B. Kieler Khanh L. Duong W. Scott Moye-Rowley J. Stacey Klutts 《Journal of microbiological methods》2013
The emerging invasive fungal pathogen Aspergillus fumigatus causes very serious infections among immunocompromised patient populations. While the genome of this pathogen has been sequenced, a major barrier to better understanding the complex biology of this eukaryotic organism is a lack of tools for efficient genetic manipulation. To improve upon this, we have generated a new gene deletion system for A. fumigatus using yeast recombinational cloning and Agrobacterium tumefaciens mediated transformation (ATMT) employing a recyclable marker system. This system reduced the time for generating a gene deletion strain in our hands by two-thirds (12 weeks to 3 weeks) using minimal human labor, and we demonstrate that it can be used to efficiently generate multiple gene deletions within a single strain. 相似文献
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Julia Candotti Nanette Christie Raphael Ployet Marja M. Mostert-O'Neill S. Melissa Reynolds Leandro Gomide Neves Sanushka Naidoo Eshchar Mizrachi Tuan A. Duong Alexander A. Myburg 《The Plant journal : for cell and molecular biology》2023,113(1):174-185
To improve our understanding of genetic mechanisms underlying complex traits in plants, a comprehensive analysis of gene variants is required. Eucalyptus is an important forest plantation genus that is highly outbred. Trait dissection and molecular breeding in eucalypts currently relies on biallelic single-nucleotide polymorphism (SNP) markers. These markers fail to capture the large amount of haplotype diversity in these species, and thus multi-allelic markers are required. We aimed to develop a gene-based haplotype mining panel for Eucalyptus species. We generated 17 999 oligonucleotide probe sets for targeted sequencing of selected regions of 6293 genes implicated in growth and wood properties, pest and disease resistance, and abiotic stress responses. We identified and phased 195 834 SNPs using a read-based phasing approach to reveal SNP-based haplotypes. A total of 8915 target regions (at 4637 gene loci) passed tests for Mendelian inheritance. We evaluated the haplotype panel in four Eucalyptus species (E. grandis, E. urophylla, E. dunnii and E. nitens) to determine its ability to capture diversity across eucalypt species. This revealed an average of 3.13–4.52 haplotypes per target region in each species, and 33.36% of the identified haplotypes were shared by at least two species. This haplotype mining panel will enable the analysis of haplotype diversity within and between species, and provide multi-allelic markers that can be used for genome-wide association studies and gene-based breeding approaches. 相似文献
715.
Brit Mollenhauer Frederick DuBois Bowman Daniel Drake Jimmy Duong Kaj Blennow Omar El‐Agnaf Leslie M. Shaw Jennifer Masucci Peggy Taylor Robert M. Umek Jill M. Dunty Chris L. Smith Erik Stoops Hugo Vanderstichele Adrian W. Schmid Marc Moniatte Jing Zhang Niels Kruse Hilal A. Lashuel Charlotte Teunissen Tanja Schubert Kuldip D. Dave Samantha J. Hutten Henrik Zetterberg 《Journal of neurochemistry》2019,149(1):126-138
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