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21.
22.
Aleš Vančura Ivana Vančurová Jan Kopecký Jaroslav Maršálek Daniel Cikánek Gabriela Basařová Vladimir Křišťan 《Archives of microbiology》1989,151(6):537-540
Synthesis of threonine dehydratase in Streptomyces fradiae was positively influenced by valine and negatively by isoleucine. However, these two amino acids had no effect on the activity of this enzyme. Synthesis of threonine dehydratase in -aminobutyrate resistant mutants of S. fradiae was pronouncedly less sensitive to the positive effect of valine and this change in regulation led to valine overproduction. Synthesis of acetohydroxy acid synthase is regulated in a similar manner to that of threonine dehydratase, however a lower level of expression was detected in -aminobutyrate resistant mutants. And again, no effect of branched-chain amino acids on acetohydroxy acid synthase activity was observed. It follows that in S. fradiae synthesis of threonine dehydratase is the main regulatory mechanism governing production and the mutual ratio of synthesized valine and isoleucine.Abbreviations -AB
-aminobutyrate
- AHAS
acetohydroxy acid synthase
- -KB
-ketobutyrate
- MNNG
N-methyl-N-nitro-N-nitrosoguanidine
- TD
threonine dehydratase
- Trans. B.
transaminase of branched-chain amino acids
- VDH
valine dehydrogenase 相似文献
23.
In the lichen genusUsnea different species ofTrebouxia-phycobionts as well as different haustorial types are known. The isolated and cultivated phycobiont ofUsnea longissima
Ach. was studied by light- and electron microscopy and resembles in cytomorphological details the type ofTrebouxia impressa
Ahmad. In addition to simple wall-to-wall contacts between the symbiotic components also intraparietal (=intrawall-)haustoria could be observed as the normal interaction type.
Frau Prof. Dr.Elisabeth Tschermak-Woess zu ihrem 70. Geburtstag gewidmet. 相似文献
24.
S. Živanović 《Human Evolution》1988,3(5):329-334
Studies of the morphological features of the temporal region of mesolithic skulls from Padina in the Iron Gate Gorge of the
Danube revealed a very prominent and large supramastoid ridge which is the most striking feature in skulls of both sexes.
Mastoid processes were larger in male skulls, but in 25% of the cases there was an overlap between the size of the processes
in male and female specimens. The mastoid ridge was prominent in both sexes. The digastric fossa was always well defined in
both sexes and in the two thirds of the skull specimens it was deep. The posterior root of the zygoma was prominent in all
the skulls, but it was better developed in the male specimens. The parietotemporal suture in both sexes rises above the level
of the pterion. There were no morphological or anthropometrical differences between the left and the right side of individual
skulls outside the limits of natural asymmetry. All these morphological characteristics of the temporal region may help in
racial and sexual diagnosis of the Mesolithic skulls from the Iron Gate Gorge. 相似文献
25.
Elizabeta Pertot Damjana Rozman Stjepan Miličić Helena Sočič 《Applied microbiology and biotechnology》1988,28(2):209-213
Summary The morphological development ofClaviceps paspali immobilized in Ca-alginate gel was examined. During consecutive reincubations, the immobilized mycelia differentiated into swollen, arthrosporoid-like cells, which never appeared during fermentation of free mycelium. Such differentiation could be connected with the improved, prologed vitality and metabolic activity of the immobilized cells. 相似文献
26.
Miroslav Flieger Jaroslav Votruba Vladimír Křen Sylvie Pažoutová Viktor Rylko Přemysl Sajdl Zdeněk Reháček 《Applied microbiology and biotechnology》1988,29(2-3):181-185
Summary Kinetic parameters of production of clavine alkaloids were evaluated in twoClaviceps purpurea strains. Mutagenesis brought about enhanced resistance of the biosynthetic system towards alkaloids. Addition of glucose into the fermentation medium altered the zero order kinetics of production to activation-inhibition kinetics. The glucose treatment allowed performance of both elymoclavine-inhibitionless and clavine alkaloid-decompositionless fermentations if a combination of fermentation and separation units in a closed loop was used.Nomenlacture
k
1
rate constant of agroclavine synthesis (mg Agro · mg Elymo/l·g DW·day for stage 1, mg Agro/g DW·day for stage 2)
-
k
2
parameter describing inhibition of agroclavine formation rate by elymoclavine (mg Elymo/l)
-
k
3
specific rate of agroclavine decay (l/g DW·day)
-
k
4
maximal specific rate of elymoclavine synthesis (stage 1, 1/g DW·day, stage 2, mg Elymo/g DW·day)
-
k
4
–
maximal specific rate of elymoclavine synthesis in stage 1 (inhibition-activation mechanism) (mg Elymo/g DW·day)
-
k
5
physiological constant describing the elymoclavine decay rate (l2/g DW·day·mg Elymo)
-
k
5
–
physiological constant describing the activation of elymoclavine biosynthesis by elymoclavine (mg Elymo/l)
-
k
6
physiological constant describing the repression of elymoclavine biosynthesis by elymoclavine (mg Elymo/l)
-
k
7
maximal specific growth rate (1/day)
-
k
8
specific rate of biomass decay (l/g DW·day)
-
A
agroclavine concentration (mg/l)
-
E
elymoclavine concentration (mg/l)
-
r
A
specific rate of agroclavine biosynthesis (mg Agro/g DW·day)
-
r
E
specific rate of elymoclavine biosynthesis (mg Elymo/g DW·day)
-
r
i
specific rate of alkaloid biosynthesis (mg alkaloid/g DW·day)
-
X
dry biomass concentration (g/l)
-
specific growth rate (1/day)
Abbreviations Agro
agroclavine
- Elymo
elymoclavine
- Chano
chanoclavine
- DW
dry weight of biomass 相似文献
27.
J. Šťovíčková J. Turková B. Urbánek V. Bažant J. Kavalírová 《Biotechnology Techniques》1988,2(2):121-126
Summary The effect was studied of the method of drying chymotrypsin attached to bead cellulose on its chemical and physical characteristics. These characteristics are not deteriorated when replacing the commonly used lyophilisation by fluid drying in the air stream. The preparations saved essentially the same proteolytic activity as well as stability even when increasing the drying air temperature to 70°C. 相似文献
28.
Krystyna Szczawinska P. A. Ferchmin Richard M. Hann Vesna A. Eterović 《Cellular and molecular neurobiology》1992,12(2):95-106
1. The electric organ of Torpedo nobiliana contained putrescine (PUT), spermidine (SPD), spermine (SPM), and cadaverine (CAD). Traces of acetylated SPD and SPM were occasionaly seen. 2. Upon fractionation of the tissue by differential centrifugation, the polyamines (PA) were found predominantly in the soluble fraction. The postsynaptic membrane fraction, containing a high concentration of acetylcholine receptor (AChR), was proportionally enriched in SPM. The molar ratio of SPM to AChR was approximately two in these membranes. 3. The effect of exogeneous PA on AChR function was studied by two methods: carbamoylcholine (CCh)-dependent 86Rb+ influx into receptor-rich membrane vesicles and [alpha-125I]bungarotoxin (Bgt) binding to the AChR. 4. SPM inhibited both ion influx and the rate of Bgt binding at concentrations above 1 mM, and therefore it appears to act as a competitive antagonist of the AChR. 5. At submicromolar concentrations, and only after preincubation with the receptor-rich membrane, SPM and PUT increased the ion influx by about 20% over control values. 6. Preincubation with 100 nM SPM did not affect the equilibrium binding of iodinated toxin or the rate of toxin binding, and therefore SPM was not uncovering new receptors. 7. By measuring the initial rate of toxin binding after different periods of preincubation with 1 microM CCh, the rate of the slow phase of receptor desensitization was determined. This rate was not changed by 100 nM SPM. 8. Although these results suggest that at low concentrations SPM is a positive modulator of the AChR, the precise mechanism of action is not determined yet. 相似文献
29.
Structure and mapping of the fosB gene. FosB downregulates the activity of the fosB promoter. 下载免费PDF全文
We have determined the genomic structure of the fosB gene and shown that it consists of 4 exons and 3 introns at positions also found in the c-fos gene. By deletion analysis we have characterized a region upstream of the TATA box which is the promoter region of the gene. Several consensus sequences have been identified, including an SRE and AP-1 binding site whose relative positions are identical to those in the 5' upstream region of the c-fos gene. We have also shown that FosB and c-Fos can downregulate the activity of the fosB promoter to a similar extent. The fosB gene is located in the [A1-B1] region of mouse chromosome 7. 相似文献
30.
Branka Brukner Dabović Dragica Radojković Predrag Minić Jovan Savić Ana Savić 《Human genetics》1992,88(6):699-700
Summary A study was undertaken to find the frequency of the F508 deletion and those of the G551D, R553X and G524X mutations among the mainly Slavic population of Serbia, Bosnia, Herzegovina, and Montenegro and compare the frequencies determined with those in other European populations. The F508 mutation was found to account for about 70% of CF genes in central Jugoslavia, where its frequency is significantly higher than elsewhere in Southern European populations. 相似文献