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31.
Small disulfide-rich proteins provide examples of simple and stable scaffolds for design purposes. The cystine-stabilized beta-sheet (CSB) motif is one such elementary structural motif and is found in many protein families with no evolutionary relationships. In this paper, we present NMR structural studies and stability measurements of two short peptides of 21 and 23 residues that correspond to the isolated CSB motif taken from a 28-residue squash trypsin inhibitor. The two peptides contain two disulfide bridges instead of three for the parent protein, but were shown to fold in a native-like fashion, indicating that the CSB motif can be considered an autonomous folding unit. The 23-residue peptide was truncated at the N-terminus. It has a well-defined conformation close to that of the parent squash inhibitor, and although less stable than the native protein, it still exhibits a high T(m) of about 100 degrees C. We suggest that this peptide is a very good starting building block for engineering new bioactive molecules by grafting different active or recognition sites onto it. The 21-residue peptide was further shortened by removing two residues in the loop connecting the second and third cysteines. This peptide exhibited a less well-defined conformation and is less stable by about 1 kcal mol(-)(1), but it might be useful if a higher flexibility is desired. The lower stability of the 21-residue peptide is supposed to result from inadequate lengths of segments connecting the first three cysteines, thus providing new insights into the structural determinants of the CSB motif. 相似文献
32.
Giang Ngan Khong Nhu Thi Le Mai Thi Pham Helene Adam Carole Gauron Hoa Quang Le Dung Tien Pham Kelly Colonges Xuan Hoi Pham Vinh Nang Do Michel Lebrun Stefan Jouannic 《PLoS genetics》2021,17(6)
The number of grains per panicle is an important yield-related trait in cereals which depends in part on panicle branching complexity. One component of this complexity is the number of secondary branches per panicle. Previously, a GWAS site associated with secondary branch and spikelet numbers per panicle in rice was identified. Here we combined gene capture, bi-parental genetic population analysis, expression profiling and transgenic approaches in order to investigate the functional significance of a cluster of 6 ANK and ANK-TPR genes within the QTL. Four of the ANK and ANK-TPR genes present a differential expression associated with panicle secondary branch number in contrasted accessions. These differential expression patterns correlate in the different alleles of these genes with specific deletions of potential cis-regulatory sequences in their promoters. Two of these genes were confirmed through functional analysis as playing a role in the control of panicle architecture. Our findings indicate that secondary branching diversity in the rice panicle is governed in part by differentially expressed genes within this cluster encoding ANK and ANK-TPR domain proteins that may act as positive or negative regulators of panicle meristem’s identity transition from indeterminate to determinate state. 相似文献
33.
Active site chemical mutagenesis of Ecballium elaterium trypsin inhibitor II: new microproteins inhibiting elastase and chymotrypsin 总被引:1,自引:0,他引:1
A Favel D Le-Nguyen M A Coletti-Previero B Castro 《Biochemical and biophysical research communications》1989,162(1):79-82
Seven microproteins analogous to Ecballium elaterium Trypsin Inhibitor II. were synthesized. The study of their inhibiting power showed a change in selectivity from trypsin to elastase for 5 of the compounds and to alpha-chymotrypsin for another one. A striking characteristic that appeared during this synthetic approach was the ability of the 28 amino acid peptides to refold and close correctly the 3 disulfide bridges, giving in each case an active compound. 相似文献
34.
Nguyen Huu Quan Vu Van Hanh Le Phuong Dung Chu Hoang Mau 《Molecular biology reports》2018,45(5):1067-1075
Molecular Biology Reports - Chitinases play the key role in hydrolysis of chitin, a huge organic carbon reservoir on earth, into monomeric sugars and their eventual conversion into valuable... 相似文献
35.
Ankit X. Sharma Ezekiel B. Quittner-Strom Young Lee Joshua A. Johnson Sarah A. Martin Xinxin Yu Jianping Li John Lu Zheqing Cai Shiuhwei Chen May-yun Wang Yiyi Zhang Mackenzie J. Pearson Andie C. Dorn Jeffrey G. McDonald Ruth Gordillo Hai Yan Dung Thai William L. Holland 《Cell reports》2018,22(7):1760-1773
36.
Van Dung Pham Sivachandiran Somasundaram Seung Hwan Lee Si Jae Park Soon Ho Hong 《Biotechnology and Bioprocess Engineering》2016,21(2):261-267
Nylon 4 is a biodegradable polymer which can be produced from the monomer of pyrrolidone. Gammaaminobutyric acid (GABA) is a precursor of pyrrolidone used for the production of bioplastics. In this study, Escherichia coli were engineered to produce gammaaminobutyric acid from glucose via an alternative novel pathway by the introduction of synthetic scaffolds. The GABA pathway constructed contained succinate dehydrogenase, succinate-semialdehyde dehydrogenase and GABA aminotransferase to redirect the Krebs cycle flux to GABA production. By introduction of a synthetic scaffold, production of 0.64 g/L GABA was achieved at 30°C and pH 6.5. Final GABA concentration was increased by 11.3% via the inactivation of competing pathways, and higher initial glucose concentration led to the enhanced final GABA concentration of 1.01 g/L. 相似文献
37.
This study is a first report of an interstitial ostracod from Southeast Asia. The ostracod species, Paracobanocythere
vietnamensis sp. n., was found in the marine interstitial environment of Phu Quoc Island, Vietnam. Thus far, three species of this genus have been described. The morphology of the carapace as well as the appendages of this new species are quite similar to Paracobanocythere
hawaiiensis and Paracobanocythere
watanabei. However, we found that they could be easily distinguished according to the morphology of the male copulatory organ. Additionally, we estimated the evolutionary distances among these three species based on nucleotide and amino acid sequences of the mitochondrial CO1 gene. Similar morphologies of carapaces and appendages, and relatively small evolutionary distances according to CO1 between Paracobanocythere
vietnamensis
sp. n. and Paracobanocythere
watanabei suggest that these two species are very closely related. 相似文献
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Annie Heitz Olga Avrutina Dung Le-Nguyen Ulf Diederichsen Jean-François Hernandez Jérôme Gracy Harald Kolmar Laurent Chiche 《BMC structural biology》2008,8(1):54