全文获取类型
收费全文 | 96503篇 |
免费 | 633篇 |
国内免费 | 884篇 |
专业分类
98020篇 |
出版年
2022年 | 29篇 |
2021年 | 55篇 |
2020年 | 40篇 |
2019年 | 40篇 |
2018年 | 11865篇 |
2017年 | 10712篇 |
2016年 | 7515篇 |
2015年 | 694篇 |
2014年 | 416篇 |
2013年 | 437篇 |
2012年 | 4416篇 |
2011年 | 12989篇 |
2010年 | 12107篇 |
2009年 | 8332篇 |
2008年 | 9923篇 |
2007年 | 11550篇 |
2006年 | 414篇 |
2005年 | 634篇 |
2004年 | 1103篇 |
2003年 | 1159篇 |
2002年 | 889篇 |
2001年 | 327篇 |
2000年 | 218篇 |
1999年 | 73篇 |
1998年 | 57篇 |
1997年 | 63篇 |
1996年 | 52篇 |
1995年 | 38篇 |
1994年 | 45篇 |
1993年 | 64篇 |
1992年 | 79篇 |
1991年 | 70篇 |
1990年 | 57篇 |
1989年 | 57篇 |
1988年 | 75篇 |
1987年 | 56篇 |
1986年 | 34篇 |
1985年 | 42篇 |
1984年 | 57篇 |
1983年 | 48篇 |
1982年 | 25篇 |
1981年 | 42篇 |
1980年 | 27篇 |
1979年 | 32篇 |
1978年 | 31篇 |
1976年 | 25篇 |
1974年 | 37篇 |
1972年 | 265篇 |
1971年 | 289篇 |
1962年 | 29篇 |
排序方式: 共有10000条查询结果,搜索用时 2 毫秒
71.
72.
73.
74.
In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization 总被引:28,自引:8,他引:20 下载免费PDF全文
In situ hybridization is used to survey the tissue-specific and developmental expression of the cloned mouse gene Sparc, coding for a protein homologous to the bovine Ca++-binding protein, osteonectin. High levels of SPARC RNA are found in osteoblasts and odontoblasts. In addition, high grain counts are associated with a variety of other cell types in the embryo and newborn mouse, including parietal endoderm, deciduum, whisker follicles (connective tissue sheath), peripheral nerve trunk, skin (dermis), and stomach (submucosa). Spatially restricted but high levels of SPARC mRNA are also seen in the adult adrenal glands, testis, and ovary. This pattern of differential gene expression demands a reassessment of the function originally proposed for osteonectin, and predicts a much wider role for the protein in a variety of biological processes. 相似文献
75.
C M Czarnecki D K Olivero A S McVey 《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1987,86(1):63-65
Plasma uric acid levels were determined in ethanol-fed poults following administration of allopurinol. In young poults, allopurinol at a dose of 50 mg/kg significantly depressed plasma uric acid levels 6 hr post-dosing. At 11 hr post-dosing, plasma uric acid levels were significantly elevated in the allopurinol-treated poults when compared with control poults. During a period of ethanol abstinence, allopurinol at a dose of 40 mg/kg significantly depressed plasma uric acid levels up to 8 hr post-dosing. At a dose of 30 mg/kg, plasma uric acid levels were similar to control values at 4 and 6 hr post-dosing. Data suggest that plasma uric acid levels can be depressed in ethanol poults when allopurinol is administered every 8 hr at a dose of 40-50 mg/kg of body weight. 相似文献
76.
Drosophila basement membrane procollagen IV. I. Protein characterization and distribution 总被引:1,自引:0,他引:1
G P Lunstrum H P B?chinger L I Fessler K G Duncan R E Nelson J H Fessler 《The Journal of biological chemistry》1988,263(34):18318-18327
A collagen was isolated from Drosophila E85, Schneider line 2L and Kc cell cultures. The purified protein was characterized and antibodies were raised against it. Immunofluorescence microscopy locates this material to the regions of basement membranes of Drosophila embryos, larvae, and adults. The molecules are mostly, or entirely, homotrimers of one polypeptide chain linked by interchain disulfide bonds. The partial amino acid sequences of a cyanogen bromide cleavage product of this chain are identical with a part of the virtual translation product of the Drosophila pro alpha 1(IV) nucleotide sequence that is reported in the accompanying paper. This gene is at Drosophila chromosome location 25C and was identified by the high homology of one part of it with the noncollagenous carboxyl terminus (NC1) of vertebrate type IV basement membrane collagens (Blumberg, B., MacKrell, A. J., Olson, P. F., Kurkinen, M., Monson, J. M., Natzle, J. E., and Fessler, J. H. (1987) J. Biol. Chem. 262, 5947-5950). In the electron microscope each molecule appears as a thread with a knob at one end, which contains the carboxyl peptide domains. The variation of flexibility of the thread was mapped along its length. Pulse-chase labeling of cell cultures showed that these molecules associate into disulfide-linked dimers and higher oligomers that can be partly separated by velocity sedimentation and are resolved by sodium dodecyl sulfate-agarose gel electrophoresis. Dimers and higher oligomers formed by overlap of the amino ends of molecules were found. Mild pepsin digestion of Drosophila embryos and larvae solubilized the corresponding disulfide-linked collagen molecules, and Staphylococcus aureus V8 protease peptide maps showed the identity of the collagen derived from animals and from cell cultures. Individual, native molecules have a sedimentation coefficient s20,w = 4.1 S, the dichroic spectrum and amino acid composition of a collagen, and a Tm = 31 degrees C. Positive in situ hybridization with a specific probe for this collagen began 6-8 h after egg laying and showed message in the locations of embryos and larvae which reacted with the antibodies. This included some prominent individual cells in the hemolymph. 相似文献
77.
C J Duncan C L Osborne J J Parsons K E McCall M J Jackson 《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1988,90(2):459-460
1. Isolated amphibian hearts and pectoris cutaneous muscles were exposed either to DNP or to caffeine, thereby producing severe myofilament damage. 2. No accompanying change in sarcolemma permeability was detected by monitoring either CK or LDH release or Procion yellow entry in the heart, or by Procion entry in amphibian skeletal muscle. 3. The findings are in contrast with mammalian cardiac and skeletal muscles, and confirm that the pathways leading to myofilament degradation and to the breakdown in sarcolemma organization are separate. 相似文献
78.
An affinity matrix was constructed by synthesis of a DNA oligonucleotide on a Teflon fiber support followed by deblocking and hybridization of the complementary strand. It was used to purify a sequence-specific binding protein at least 100-fold to near homogeneity. This matrix is easily fabricated on an automated DNA synthesizer, contains high levels of attached DNA, and has superior mechanical properties. It should be generally useful for affinity chromatography of DNA binding proteins. 相似文献
79.
The kinetics of haemolysis of rabbit erythrocytes byCroton tiglium lectin was studied as a function of concentration of the lectin and erythrocytes. The length of the prelytic period decreased with increasing lectin concentrations, indicating that the secondary events at the membrane which follow the binding of the lectin to cell surface carbohydrate receptors are accelerated at higher surface concentrations of the lectin. The rate or extent of haemolysis was not affected by the inclusion of ions like K+, Ca2+ and Mg2+ in the medium or by the substitution of ionic medium by a non-ionic medium. The inhibition of haemagglutination and haemolysis of rabbit red cells byCroton tiglium lectin by antilectin rabbit serum was observed. A possible mechanism of haemolysis by the lectin is discussed. 相似文献
80.
N-Acetyl-D-galactosamine in β-linkage being ubiquitous in cell surface glycoproteins, their interaction with lectins specific for this sugar moiety may be a significant event in cell adhesion phenomena. This article discusses the common β-N-acetyl galactosamine-specific lectins, with particular stress on the lectin from winged beans (Psophocarpus tetragonolobus). 相似文献