膜联蛋白A1与恶性肿瘤侵袭转移

膜联蛋白A1(Annexin A1,ANXA1,lipocortin I)是膜联蛋白超家族中的一员,它参与细胞信号转导、分化及凋亡等多种重要的生命过程.近年来的研究表明其表达水平在不同肿瘤组织中有差异,在同一肿瘤的不同类型中有显著变化,可能与肿瘤的侵袭转移相关,如乳腺癌、胃肠癌、肝癌、头颈部肿瘤、前列腺癌等.本文结合ANXA1的基本结构及生物学特性对以上研究的进展作一综述.     

广西苦苣苔科植物濒危程度和优先保护序列研究

在资料收集和实地调查的基础上,以已知广西现存分布的苦苣苔科24属236种珍稀特有植物(截至2018年底)为研究对象,通过对其评价指标定量化和权重分配处理,计算各物种的“濒危系数”和“优先保护值”,确定其濒危等级及优先保护次序。结果表明:236种广西苦苣苔科植物中有58种处于极危状态(CR),70种处于濒危状态(EN),72种处于易危状态(VU),31种处于近危状态(NT),5种处于无危状态(LC); 优先保护值范围为0.429～0.952,其中被划为I级保护植物107种,Ⅱ级保护植物75种,Ⅲ级保护植物28种,Ⅳ级保护植物26种。该研究结果与国家级、省级植物保护的种类和级别以及根据文献和IUCN物种红色名录所综合得出的濒危等级情况在部分种类上存在一定差异,但与目前广西分布的苦苣苔科植物所受保护与受破坏的现象基本一致,大部分苦苣苔科植物在广西所遭受的威胁程度在进一步加强,应当引起重视。     

SIK2参与TGFβ信号通路的调节

SIK2是调节糖脂代谢最重要的激酶之一.前期研究表明SIK2的同源家族成员SIK1可调控TGFβ信号通路的活性,但SIK2对TGFβ信号通路的调控作用尚无报导.本研究中我们检测了TGFβ通路对SIK2的调节作用以及SIK2对该通路活性的调控.结果表明,TGF β刺激可诱导SIK2的mRNA表达上调|转染结合报告酶活性分析显示,SIK2可反式激活含TGFβ信号通路反应元件（CAGA）的人工启动子驱动的Luc报告基因的表达|在有TGFβ存在时甚至增强其表达|Western印迹分析表明TGFβ1诱导下敲低SIK2 使p21蛋白表达水平下降.本研究结果显示SIK2可被TGFβ诱导表达,是一个新的TGFβ信号通路正调控因子.     

cis-Expression QTL analysis of established colorectal cancer risk variants in colon tumors and adjacent normal tissue

Genome-wide association studies (GWAS) have identified 19 risk variants associated with colorectal cancer. As most of these risk variants reside outside the coding regions of genes, we conducted cis-expression quantitative trait loci (cis-eQTL) analyses to investigate possible regulatory functions on the expression of neighboring genes. Forty microsatellite stable and CpG island methylator phenotype-negative colorectal tumors and paired adjacent normal colon tissues were used for genome-wide SNP and gene expression profiling. We found that three risk variants (rs10795668, rs4444235 and rs9929218, using near perfect proxies rs706771, rs11623717 and rs2059252, respectively) were significantly associated (FDR q-value ≤0.05) with expression levels of nearby genes (<2 Mb up- or down-stream). We observed an association between the low colorectal cancer risk allele (A) for rs10795668 at 10p14 and increased expression of ATP5C1 (q = 0.024) and between the colorectal cancer high risk allele (C) for rs4444235 at 14q22.2 and increased expression of DLGAP5 (q = 0.041), both in tumor samples. The colorectal cancer low risk allele (A) for rs9929218 at 16q22.1 was associated with a significant decrease in expression of both NOL3 (q = 0.017) and DDX28 (q = 0.046) in the adjacent normal colon tissue samples. Of the four genes, DLGAP5 and NOL3 have been previously reported to play a role in colon carcinogenesis and ATP5C1 and DDX28 are mitochondrial proteins involved in cellular metabolism and division, respectively. The combination of GWAS findings, prior functional studies, and the cis-eQTL analyses described here suggest putative functional activities for three of the colorectal cancer GWAS identified risk loci as regulating the expression of neighboring genes.     

Genetic Diversity Within and Among Populations of Viola tenuicornis with Reference to Sampling Strategies

Genetic diversity and population differentiation of Viola tenuicornis W. Beck. were studied in nine populations in Beijing using horizontal starch gel electrophoresis. Allozyme data for 11 loci of 8 enzymes indicated high level of genetic variability in this region with P=72.7％, A=2.4 and H e=0.243. In contrast, the population differentiation was lower than the average of other perennial herb with F st=0.196. It was found that distances among populations had little effect on population differentiation. A sample of 30 individuals could well represent a population, but less than 20 individuals per population might lead to significant bias in the estimation of genetic parameters, in particular the values of A and H e.     

低密度脂蛋白受体相关蛋白2结合蛋白在心脏特异转基因小鼠中引起的心脏功能代偿

目的建立心脏特异表达的低密度脂蛋白受体相关蛋白2结合蛋白(Lrp2bp)转基因小鼠,研究该基因在心肌病发病中的作用。方法克隆鼠源Lrp2bp基因入α-MHC启动子下游,构建a-MHC-Lrp2bp表达载体,显微注射法建立Lrp2bp转基因小鼠。PCR鉴定转基因首建鼠的基因型。Westernblotting鉴定Lrp2bp在心脏中的表达,心脏超声检测转基因鼠及野生型小鼠心脏结构和功能,透射电镜观察心肌细胞的超微结构改变。结果得到了4个Lrp2bp转基因品系,其中3个品系心脏Lrp2bp蛋白表达量与同龄野生型鼠相比明显增加。1M龄转基因小鼠与同窝阴性对照小鼠相比,心壁变厚,心腔变大,射血分数和短轴缩短率下降。结论心脏特异表达的Lrp2bp基因能引起心肌肥厚表型,可能是参与心肌代偿性肥厚的基因之一。     

上海世界博览会园区内及周边地区鸟类多样性及其影响因子

2006年3-9月,对上海世博园区及周边地区鸟类资源进行普查,并对鸟类数量、种类和环境因子相关性进行研究。结果表明,该区域内现有鸟类10目27科67种,其群落多样性指数为3.46。运用概率统计方法分析了鸟类群落结构和分布特征,并将鸟类群落指数与园林绿地的8项环境指标进行分析,表明鸟类的种数、数量以及多样性指数与公园的面积、水域面积、形状指数、生境种类呈极显著相关;鸟类多样性、均匀性指数与树种总数呈显著相关;鸟类种数与环境中的位置条件有关。同时,就世博园区绿地建设中的引鸟工程,提出了5点建议。     

崖白菜属的一个新异名(英文)

根据对崖白菜的腊叶标本和武汉植物园栽培的崖白菜活体植物的研究,结合崖白菜的野外调查,认为全缘叶崖白菜应作为崖白菜的异名处理。     

Nucleotide diversity of 11S seed storage protein gene and its implications for ecological adaptation of Oryza nivara

The mechanism by which a new species arises and adapts to its environment is a fundamental question in evolutionary biology.Seed characteristics such as seed size and nutrient composition are important fitness-related traits and have been shown to vary greatly among populations and species.However,the significance of variation in seed traits in plant adaptation and speciation remains unclear.We carried out a population genetic study on nucleotide variation of one 11S seed storage protein gene(Pss) of Oryza rufipogon Griff,and O.nivara Sharma Shastry,two closely related wild rice species.By comparatively examining the genetic variation pattern of the regulatory and coding regions of Pss and fragments of six reference loci across different chromosomes,we found significantly lower polymorphisms at coding regions of the gene(PssI) in O.nivara relative to O.rufipogon.Neutrality tests indicated that the reduction of polymorphisms at PssI in O.nivara was caused by positive selection rather than population demography,implying a role of selection on the 11S seed protein gene.Further phylogenetic and principal component analyses also support the hypotheses that the origin of O.nivara was associated with the adaptive divergence on the coding region of Pss.It is most likely that higher reproductive effort would be favored when O.nivara arose from O.rufipogon populations and adapted to the environment change.