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31.
Abstract This paper demonstrates the changes in structure, floristics and forest floor light regimes across the boundary between open forest and rainforest at Kirrama in northeastern Queensland. Hemispherical photographs of the canopy were used to estimate spatial and temporal variations in potential (clear-sky) direct and diffuse photosynthetic photon flux density (PPFD = 400–700 nm) across the boundary. Five vegetation zones were identified across the boundary: (A) open forest, (B) tall open forest, (C) tall open forest with a rainforest under-storey, (D) young rainforest, and (E) mature rainforest. During the summer (September–March) direct PPFD declined semi-exponentially across the boundary, while in the winter (April–August), the decline was more linear. However, the decline in diffuse PPFD across the boundary was linear throughout the year. Mean per cent grass cover was positively correlated and mean per cent shrub cover negatively correlated with annual average total PPFD across the boundary. Mean percentages of diffuse radiation relative to that above the forest (diffuse site factors) ranged from 9.8% in the mature rainforest to 66.4% in the open forest, while mean percentages of direct radiation relative to that above the forest (direct site factors) ranged from 2.9–38.3% at the same sites, respectively. Daily PPFD in the rainforest understorey is probably insufficient for the shade intolerant (pioneer) species which prefer the more open environments on the rainforest margin where light intensities and soil temperatures are higher. Towards the rainforest end of zone C, light conditions appear to be similar to those experienced within small treefall gaps in rainforests. Typically, such light conditions are preferred by shade tolerant (primary) tree species that are unable to grow and reproduce successfully in the adjacent shaded understorey and to compete with the fast growing pioneer tree species in the more open end of zone C. 相似文献
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Payam Vahmani William J. Meadus Maria L.P. da Silva Alec D. Mitchell Cletos Mapiye Pascale Duff David C. Rolland Michael E.R. Dugan 《Biochemistry and Biophysics Reports》2016
Beef fat is a natural source of trans (t) fatty acids, and is typically enriched with either t10-18:1 or t11-18:1. Little is known about the bioactivity of individual t-18:1 isomers, and the present study compared the effects of t9-18:1, cis (c)9-18:1 and trans (t)-18:1 fractions isolated from beef fat enriched with either t10-18:1 (HT10) or t11-18:1 (HT11). All 18:1 isomers resulted in reduced human liver (HepG2) cell viability relative to control. Both c9-18:1 and HT11were the least toxic, t9-18:1had dose response increased toxicity, and HT10 had the greatest toxicity (P<0.05). Incorporation of t18:1 isomers was 1.8–2.5 fold greater in triacylglycerol (TG) than phospholipids (PL), whereas Δ9 desaturation products were selectively incorporated into PL. Culturing HepG2 cells with t9-18:1 and HT10 increased (P<0.05) the Δ9 desaturation index (c9–16:1/16:0) compared to other fatty acid treatments. HT10 and t9-18:1 also increased expression of lipogenic genes (FAS, SCD1, HMGCR and SREBP2) compared to control (P<0.05), whereas c9-18:1 and HT11 did not affect the expression of these genes. Our results suggest effects of HT11 and c9-18:1 were similar to BSA control, whereas HT10 and t-9 18:1 (i.e. the predominant trans fatty acid isomer found in partially hydrogenated vegetable oils) were more cytotoxic and led to greater expression of lipogenic genes. 相似文献
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Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterial responses 总被引:14,自引:0,他引:14
Wyllie DH Kiss-Toth E Visintin A Smith SC Boussouf S Segal DM Duff GW Dower SK 《Journal of immunology (Baltimore, Md. : 1950)》2000,165(12):7125-7132
Members of the Toll-like receptor (TLR) family are components of the mammalian anti-microbial response, signaling with a domain closely related to that of IL-1 receptors. In this report the expression and function of TLR1, a TLR of unknown function, are examined. TLR1 is expressed by monocytes, as demonstrated using a novel mAb. Monocytes also express TLR2. TLR1 transfection of HeLa cells, which express neither TLR1 nor TLR2, was not sufficient to confer responsiveness to several microbial extracts. However, cotransfection of TLR1 and TLR2 resulted in enhanced signaling by HeLa cells to soluble factors released from Neisseria meningitidis relative to the response with either TLR alone. This phenomenon was also seen with high concentrations of some preparations of LPS. The N. meningitidis factors recognized by TLR1/TLR2 were not released by N. meningitidis mutant in the LpxA gene. Although LpxA is required for LPS biosynthesis, because cooperation between TLR1 and TLR2 was not seen with all LPS preparations, the microbial component(s) TLR1/2 recognizes is likely to be a complex of LPS and other molecules or a compound metabolically and chemically related to LPS. The functional IL-1R consists of a heterodimer; this report suggests a similar mechanism for TLR1 and TLR2, for certain agonists. These data further suggest that mammalian responsiveness to some bacterial products may be mediated by combinations of TLRs, suggesting a mechanism for diversifying the repertoire of Toll-mediated responses. 相似文献
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A pathogenicity determinant maps to the N‐terminal coat protein region of the Pepino mosaic virus genome 下载免费PDF全文
Celia R. A. Duff‐Farrier Andy M. Bailey Neil Boonham Gary D. Foster 《Molecular Plant Pathology》2015,16(3):308-315
Pepino mosaic virus (PepMV) poses a worldwide threat to the tomato industry. Considerable differences at the genetic level allow for the distinction of four main genotypic clusters; however, the basis of the phenotypic outcome is difficult to elucidate. This work reports the generation of wild‐type PepMV infectious clones of both EU (mild) and CH2 (aggressive) genotypes, from which chimeric infectious clones were created. Phenotypic analysis in three solanaceous hosts, Nicotiana benthamiana, Datura stramonium and Solanum lycopersicum, indicated that a PepMV pathogenicity determinant mapped to the 3′‐terminal region of the genome. Increased aggression was only observed in N. benthamiana, showing that this factor is host specific. The determinant was localized to amino acids 11–26 of the N‐terminal coat protein (CP) region; this is the first report of this region functioning as a virulence factor in PepMV. 相似文献
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Six novel genes encoding proteins with the interleukin (IL)-1 fold have been identified recently. The classical family members are involved in inflammatory signaling. Previous work has placed the novel genes close to or within the same cluster as IL1A, IL1B, and IL1RN, which occupy an approximately 400-kb interval on chromosome 2. We have combined the incomplete public database sequence with our own sequence to generate a reference sequence and map that encompass all of the novel genes, allowing determination of the gene structures, precise localization of exons, and determination of distances between conventional SNP and microsatellite markers. Gene order from centromere to telomere is IL1A-IL1B-IL1F7-IL1F9-IL1F6-IL1F8-IL1F5-IL1F10-IL1RN, of which only IL1A, IL1B, and IL1F8 are transcribed towards the centromere. The gene order relates to the evolutionary relationship between the genes. Key features of exon boundaries are conserved. There is no evidence for other IL-1 family members within the cluster. 相似文献
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Properties of Hamster Embryo Fibroblasts Transformed In Vitro After Exposure to Ultraviolet-Irradiated Herpes Simplex Virus Type 2 总被引:23,自引:30,他引:23 下载免费PDF全文
An in vitro method which led to the transformation of hamster embryo fibroblasts after exposure to herpes simplex virus type 2 (HSV-2) inactivated with ultraviolet irradiation is described. The transformed cells (333-8-9) produced tumors when inoculated into newborn Syrian hamsters but not when injected into weanling Syrian hamsters of the same LSH inbred strain. However, after one in vivo passage, the 333-8-9 cells became highly oncogenic in weanling hamsters. No infectious virus was recovered from these cells. Herpes simplex virus antigens were detected in the transformed cells by the indirect immunofluorescence technique. Sera from tumor-bearing hamsters contained antibody with highly specific neutralizing activity against HSV-2. These studies indicate the continued involvement of the HSV-2 genome in an oncogenic cell line. 相似文献