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排序方式: 共有264条查询结果,搜索用时 187 毫秒
51.
52.
Natalia Stach Magdalena Kalinska Michal Zdzalik Radoslaw Kitel Abdulkarim Karim Karol Serwin Wioletta Rut Katrine Larsen Abeer Jabaiah Magdalena Firlej Benedykt Wladyka Patrick Daugherty Henning Stennicke Marcin Drag Jan Potempa Grzegorz Dubin 《Structure (London, England : 1993)》2018,26(4):572-579.e4
53.
Whole-cell membrane currents were recorded from olfactory receptor neurons from the neotenic salamander Necturus maculosus. Cyclic nucleotides, released intracellularly by flash photolysis of NPE-caged cAMP or NPE-caged cGMP, activated a transient
chloride current. The chloride current could be elicited at constant voltage in the absence of extracellular Ca2+ as well as in the presence of 3 mm intracellular Ca2+, suggesting that the current did not require either voltage or Ca2+ transients for activation. The current could be elicited in the presence of the protein kinase inhibitors H-7 and H-89, and
in the absence of intracellular ATP, indicating that activation was independent of protein kinase A activity. These results
suggest that Necturus olfactory receptor neurons contain a novel chloride ion channel that may be directly gated by cyclic nucleotides.
Received: 12 November 1996/Revised: 4 April 1997 相似文献
54.
Anna Małolepszy Terry Mun Niels Sandal Vikas Gupta Manu Dubin Dorian Urbański Niraj Shah Asger Bachmann Eigo Fukai Hideki Hirakawa Satoshi Tabata Marcin Nadzieja Katharina Markmann Junyi Su Yosuke Umehara Takashi Soyano Akira Miyahara Shusei Sato Makoto Hayashi Jens Stougaard Stig U. Andersen 《The Plant journal : for cell and molecular biology》2016,88(2):306-317
Long terminal repeat (LTR) retrotransposons are closely related to retroviruses, and their activities shape eukaryotic genomes. Here, we present a complete Lotus japonicus insertion mutant collection generated by identification of 640 653 new insertion events following de novo activation of the LTR element Lotus retrotransposon 1 (LORE1) ( http://lotus.au.dk ). Insertion preferences are critical for effective gene targeting, and we exploit our large dataset to analyse LTR element characteristics in this context. We infer the mechanism that generates the consensus palindromes typical of retroviral and LTR retrotransposon insertion sites, identify a short relaxed insertion site motif, and demonstrate selective integration into CHG‐hypomethylated genes. These characteristics result in a steep increase in deleterious mutation rate following activation, and allow LORE1 active gene targeting to approach saturation within a population of 134 682 L. japonicus lines. We suggest that saturation mutagenesis using endogenous LTR retrotransposons with germinal activity can be used as a general and cost‐efficient strategy for generation of non‐transgenic mutant collections for unrestricted use in plant research. 相似文献
55.
We have sequenced a lysine tRNA from mosquito mitochondria that has the anticodon CUU. The preponderance of AAA lysine codons in insect mitochondrial genes, the parsimonious organization of the genomes, and the fact that this tRNA is a major component of the mosquito mitochondrial tRNA complement, lead us to suggest that the CUU anticodon recognizes AAC and AAA codons. 相似文献
56.
D T Dubin T N Jones G R Cleaves 《Biochemical and biophysical research communications》1974,56(2):401-406
A novel low molecular weight (“3 SE”) RNA associated with hamster cell mitochondria has been partially characterized. It was present at approx. 1:1 molar ratio with structural mitochondrial ribosomal RNA; it was unmethylated; and it resembled other mitochondrial RNA fractions in having a low content of G + C. These findings support the idea that 3 SE RNA is a mitochondrial equivalent of 5 S ribosomal RNA. 相似文献
57.
Degree of methylation of mitochondrial ribosomal RNA 总被引:1,自引:0,他引:1
58.
Tim H. Parmley Norman H. Dubin David A. Blake Theodore M. King 《Journal of medical primatology》1983,12(1):41-48
This paper describes recent success in performing hysterosalpingography in cynomolgus monkeys. The animals were anesthesized, and a needle was manipulated through the cervical canal into the endometrial cavity for injection of contrast material. Difficulties in threading the needle through the canal were due to several blind pouches formed by folds in the cervical mucosa. The uterine lumen and both tubes could be visualized in over 75% of the cases. The study demonstrates that the hysterosalpingography technique can be performed satisfactorily in cynomolgus monkeys. 相似文献
59.
The sequences of three transfer RNAs from mosquito cell mitochondria, tRNAUCGArg, tRNAGUCAsp, and tRNAGAUIle, determined using a combination of rapid ladder and fingerprinting procedures are reported. These were compared with hamster mitochondrial tRNAUCGArg and tRNAGUCAsp determined similarly, and a bovine mitochondrial tRNAGAUIle determined using a somewhat different approach. The primary sequences of the mosquito tRNAs were 35 to 65% homologous to the corresponding mammalian mitochondrial species, and bore little homology to “conventional” (bacterial or eucaryotic cytoplasmic) tRNA. The modification status of the mosquito mitochondrial tRNAs resembled that of mammalian mitochondrial tRNA. The results contribute to the generalization that metazoan mitochondrial tRNA constitutes a distinctive, albeit loosely structured, phylogenetic group. 相似文献
60.
Methylation patterns of mycoplasma transfer and ribosomal ribonucleic acid. 总被引:2,自引:0,他引:2
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The methylation patterns of transfer and ribosomal ribonucleic acid (RNA) from two mycoplasmas, Mycoplasma capricolum and Acholeplasma laidlawii, have been examined. The transfer RNA from the two mycoplasmas resembled that of other procaryotes in degree of methylation and general diversity of methylated nucleotides, and bore particular resemblance to Bacillus subtilis transfer RNA. The only unusual feature was the absence of m5U from M. capricolum transfer RNA. The methylation patterns of the mycoplasma 16S RNAs were also typically procaryotic, retaining the methylated residues previously shown to be highly conserved among eubacterial 16S RNAs. The mycoplasma 23S RNA methylation patterns were, on the other hand, quite unusual. M. capricolum 23S RNA contained only four methylated residues in stoichiometric amounts, all of which were ribose methylated. A. laidlawii 23S RNA contained the same ribose-methylated residues, plus in addition approximately six m5U residues. These findings are discussed in relation to the phylogenetic status of mycoplasma, as well as the possible role of RNA methylation. 相似文献