全文获取类型
收费全文 | 5623篇 |
免费 | 378篇 |
国内免费 | 514篇 |
专业分类
6515篇 |
出版年
2024年 | 20篇 |
2023年 | 105篇 |
2022年 | 186篇 |
2021年 | 322篇 |
2020年 | 239篇 |
2019年 | 250篇 |
2018年 | 246篇 |
2017年 | 189篇 |
2016年 | 255篇 |
2015年 | 352篇 |
2014年 | 448篇 |
2013年 | 483篇 |
2012年 | 508篇 |
2011年 | 456篇 |
2010年 | 298篇 |
2009年 | 259篇 |
2008年 | 291篇 |
2007年 | 257篇 |
2006年 | 221篇 |
2005年 | 205篇 |
2004年 | 164篇 |
2003年 | 131篇 |
2002年 | 119篇 |
2001年 | 68篇 |
2000年 | 59篇 |
1999年 | 75篇 |
1998年 | 47篇 |
1997年 | 27篇 |
1996年 | 44篇 |
1995年 | 35篇 |
1994年 | 25篇 |
1993年 | 19篇 |
1992年 | 18篇 |
1991年 | 20篇 |
1990年 | 17篇 |
1989年 | 12篇 |
1988年 | 9篇 |
1987年 | 10篇 |
1986年 | 9篇 |
1985年 | 8篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1981年 | 2篇 |
1979年 | 4篇 |
排序方式: 共有6515条查询结果,搜索用时 15 毫秒
971.
972.
973.
974.
MG132 Induces Expression of Monocyte Chemotactic Protein‐Induced Protein 1 in Vascular Smooth Muscle Cells 下载免费PDF全文
975.
976.
Contrasting responses in the growth and energy utilization properties of sympatric Populus and Salix to different altitudes: implications for sexual dimorphism in Salicaceae 下载免费PDF全文
An interesting ecological and evolutionary puzzle arises from the observations of male‐biased sex ratios in genus Populus, whereas in the taxonomically related Salix, females are generally more dominant. In the present study, we combined results from a field investigation into the sex ratios of the Salicaceous species along an altitudinal gradient on Gongga Mountain, and a pot experiment by monitoring growth and energy utilization properties to elucidate the mechanisms governing sexual dimorphism. At middle altitudes 2000 and 2300 m, the sex ratios were consistent with a 1:1 equilibrium in sympatric Populus purdomii and Salix magnifica. However, at the lower and higher ends of the altitudinal gradient, skewed sex ratios were observed. For example, the male:female ratios were 1.33 and 2.36 in P. purdomii at 1700 and 2600 m respectively; for S. magnifica the ratio was 0.62 at 2600 m. At 2300 m, the pot‐grown seedlings of both species exhibited the highest biomass accumulation and total leaf area, simultaneously with the balanced sex ratios in the field. At 3300 m, the specific leaf area in male P. purdomii was 23.9% higher than that of females, which may be the morphological cause for the observed 19.3% higher nitrogen allocation to Rubisco, and 20.6% lower allocation to cell walls. As such, male P. purdomii showed a 32.9% higher foliar photosynthetic capacity, concomitant with a 12.0% lower construction cost. These properties resulted in higher photosynthetic nitrogen‐ and energy‐use efficiencies, and shorter payback time (24.4 vs 40.1 days), the time span that a leaf must photosynthesize to amortize the carbon investment. Our results thus suggested that male P. purdomii evolved a quicker energy‐return strategy. Consequently, these superior energy gain‐cost related traits and the higher total leaf area contributed to the higher growth rate and tolerance in stress‐prone environments, which might, in part, shed new light on the male‐biased sex ratios in Populus. However, no significant sexual difference was observed in S. magnifica for all the above parameters, thereby implying that the female‐biased sex ratios in Salix cannot be explained in terms of the energy‐use properties studied here. 相似文献
977.
Reactive carbonyl species methylglyoxal (MG) has always been regarded as a cytotoxic metabolite, but now is emerging to function as signal molecule in plants. However, whether MG can induce salt tolerance is elusive. In this study, treatment of wheat seeds with NaCl reduced seed germination, plant height, root length, fresh weight, and dry weight, indicating the inhibitive effects of NaCl on seed germination and seedling growth. The inhibitive effects of NaCl were alleviated by applying exogenous MG, but aggravated by the MG scavenger N-acetyl-L-cysteine (NAC), suggesting that MG could induce the salt tolerance of wheat. In addition, MG increased glyoxalase I and glyoxalase II activities and decreased endogenous MG content in wheat seedlings under NaCl stress, whereas coapplication of NAC weakened glyoxalase activity and enhanced the endogenous MG level. Also, MG activated superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase activities; increased glutathione and ascorbic acid levels; and decreased superoxide radical production and H2O2 and malondialdehyde contents under NaCl stress, while NAC reversed these physiological parameters. Furthermore, MG also induced the accumulation of proline, glycine betaine, and soluble sugar under NaCl stress, whereas this accumulation was weakened by NAC. This work reported for the first time that MG could induce the salt tolerance of wheat, and the acquisition of this salt tolerance was involved in the activation of the glyoxalase system and antioxidant system, as well as the accumulation of osmolytes. 相似文献
978.
979.
Bacteria have been actively regulating cycles of various elements in the environment. To explore the potential bacterial role in gold biogeochemical cycling, this study analyzed the bacterial diversity of mine rock (MR) and surface soil (SS) samples from Linglong gold mine using 16S rRNA gene clone library analysis and cultivation method. From MR, 24 operational taxonomic units (OTUs) were identified from MR, covering 3 phyla and 18 genera. Meanwhile, 24 OTUs were identified from SS, including 4 phyla and 18 genera. Compared with 16S rRNA gene clone library analysis, 28 aerobic and 34 anaerobic isolates were obtained, whereas 26 aerobic and 71 anaerobic strains were isolated from SS. The cultivable bacteria were affiliated with Firmicutes, Proteobacteria and Actinobacteria phyla, and dominated by Firmicutes. These results underscore the high level of bacterial diversity in the gold mine. Our study provides information on the microbial diversity in Linglong gold mine and sheds light on the existence and potential function of bacteria in the gold biogeochemical cycling. 相似文献
980.
Yuanyun Ao Xiaoyue Li Lili Li Xiaolu Xie Dong Jin Jiemei Yu Shan Lu Zhaojun Duan 《中国科学:生命科学英文版》2017,60(12):1348-1356
Bocaparvovirus (BOV) is a genetically diverse group of DNA viruses and a possible cause of respiratory, enteric, and neurological diseases in humans and animals. Here, two highly divergent BOVs (tentatively named as Himalayan marmot BOV, HMBOV1 and HMBOV2) were identified in the livers and feces of wild Himalayan marmots in China, by viral metagenomic analysis. Five of 300 liver samples from Himalayan marmots were positive for HMBOV1 and five of 99 fecal samples from these animals for HMBOV2. Their nearly complete genome sequences are 4,672 and 4,887 nucleotides long, respectively, with a standard genomic organization and containing protein-coding motifs typical for BOVs. Based on their NS1, NP1, and VP1, HMBOV1 and HMBOV2 are most closely related to porcine BOV SX/1-2 (approximately 77.0%/50.0%, 50.0%/53.0%, and 79.0%/54.0% amino acid identity, respectively). Phylogenetic analysis of these three proteins showed that HMBOV1 and HMBOV2 formed two distinctly independent branches in BOVs. According to these results, HMBOV1 and HMBOV2 are two different novel species in the Bocaparvovirus genus. Their identification expands our knowledge of the genetic diversity and evolution of BOVs. Further studies are needed to investigate their potential pathogenicity and their impact on Himalayan marmots and humans. 相似文献