The influence of EDTA on the mutagenic activity of ethyl methanesulphonate (EMS) inArabidopsis thaliana

Nebyl prokázán sensibilizující ú?inek EDTA na muta?ní aktivitu EMS uArabidopsis thaliana.     

Morphology and function of the Golgi apparatus

The polymorphism of the dictyosomes in the root meristeme ofFagopyrum is connected with their various functions in secretory processes and cell differentiation. The dictyosomes containing vesicular dilatations of the cisternae, which in this object occur more frequently than in others, probably participate in a similar way as the Golgi apparatus of the animal cell in the formation of lysozomes, in the formation of elements belonging to the group of dense bodies analogical lysozomes. These bodies are present in large numbers in the cytoplasm of cells, containing dictyosomes with vesicular dilatations. The other forms of the dictyosomes reveal indications of their participation in the production of the carbohydrate material of the cell walls, like most dictyosomes of other plant objects. However, no fusion of the Golgi vesicles with the plasmalemma was observed. According to their morphological appearance the typical forms of dictyosomes were classified on the basis of their relationship to secretory processes. Simultaneously the morphology and function of the Golgi apparatus was compared in the animal and plant cell. Several morphological varieties of the dictyosomes of plant cells, observed after the action of pathogenic factors and the effect of the fixation procedures, were also noticed in small quantities in the cells of the investigated objects.     

Importance de l'étude de la répartition écologique pour la taxonomie des éponges calcaires

Résumé 1. Les travaux deSarà (1953) et deBurton (1963) ont remis en question la valeur systématique des caractères morphologiques dans la classification des Eponges Calcaires, et ont proposé de considérer de nombreuses formes connues comme des variétés individuelles et régionales d'espèces très plastiques et variables. Nous avons essayé de préciser, par l'observation directe en plongée libre, la distribution et les préférences écologiques des Calcaires, de définir ainsi les limites naturelles de leurs populations, et d'étudier ensuite la variabilité des caractères morphologiques à l'intérieur de ces populations.2. Nous exposons ici l'exemple des espèces des genresClathrina etAscandra, étudiées en Adriatique, dans les régions de Marseille et de Roscoff. Leur distribution est en rapport direct et très précis avec l'exposition aux vagues et à la lumière, à tel point que sur une surface réduite plusieurs espèces se succèdent et se remplacent en fonction de l'exposition de l'endroit. Dans la règion de Roscoff cette distribution est plus large ce qui est dû aux effets des marées, mais les zones écologique préférentielles pour les espèces deClathrina ont pu être établies en fonction de l'importance de l'apport des sédiments par les eaux côtières.3. La possibilité de définir des populations dont la distribution est très précisemment délimitée malgré une coexistence dans un espace réduit, et qui ne sont pas réliées par les formes transitoires, permet d'éliminer l'hypothèse de la cospécificité de ces populations. Elle permet également d'établir les variations morphologiques possibles au sein d'une espèce, de retenir les caractères significatifs pour la classification, et finalement de distinguer les espèces qui se ressemblent morphologiquement. Dans le cas d'animaux qui, commeClathrina, possèdent très peu de caractères utilisables en systématique, c'est la seule façon de résoudre les problèmes de leur taxonomie.

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The importance of research on the ecological distribution for the taxonomy of calcareous sponges

The particular difficulties of the taxonomy of calcareous sponges are due to their great plasticity and their capacity for adaptation which obscure the natural limits of species. An attempt was made to define the natural populations of the Mediterranean species of the generaClathrina andAscandra through a study of their ecological distributions. The populations not connected by the transitory forms were defined by their ecological preferences, and, as they are found in a close cohabitation, were treated as having the specific values. The variability of different morphological features is discussed, the features significant for taxonomy are pointed out and the morphologically close species are defined.

     

Stand microclimate and physiological activity of tree leaves in an oak-hornbeam forest

Summary In an uneven-aged, multi-species oak-hornbeam forest at Báb, SW Slovakia (former IBP Forest Research Site), a series of micrometeorological and ecophysiological measurements started in 1985. The aims of the work are to improve understanding of physiological processes (photosynthesis, respiration, and transpiration) of adult trees and stand microclimate, to collect data for simulation of the canopy (stand) photosynthesis and for ecological synthesis of the functioning of the forest ecosystem. In this paper, photosynthetically active radiation (PAR), air temperature (AT) and relative humidity (RH), wind speed (WS), and CO₂ concentration ([CO₂]) in and above the forest are characterized for the fully leaved season, using diurnal courses, vertical profiles and isodiagrams (isopleths). Approximately 50% of incident PAR was absorbed by the upper 4–5 m layer of leaves and only approximately 5% or less penetrated to the forest floor. Vertical gradients of AT and RH were generally low, but large differences in diurnal ranges of AT and RH were observed between vertical levels. The upper leaf canopy greatly reduced WS, and at a height of about 14 m above the ground it was close to zero. The highest diurnal [CO₂] maximum and variations occurred at 1 m above the ground, and the lowest above the forest. In good light conditions in the forest, the entire leaf canopy (overstorey and understorey canopy) is a large sink of CO₂. At night the forest stand is a source of CO₂, the largest internal source being the soil and forest floor.     

Macrophages of the rat thymus after cyclosporin treatment. Histochemical, enzymehistochemical and immunohistochemical study

Young adult Wistar rats received 40 mg/kg of cyclosporin perorally for 21 days. Cyclosporin induced almost total disappearance of thymic medulla, whereas the cortex remained preserved. Although the density of cortical macrophages did not change significantly, their characteristics altered markedly and they became enlarged and rounded. In addition to an increase in acid phosphatase and nonspecific esterase activities, cortical macrophages developed very strong succinic dehydrogenase and chloroacetate esterase activities and a fine, granular, aldehyde fuchsin-positive cytoplasmic content. However, these cytoplasmic granules were PAS-negative and were not sudanophilic. Cortical macrophages retained their normal antigenic properties (which were studied by the use of ED1, ED2 and R-MC 41 monoclonal antibodies). Phagocytic cells in the remaining medullary islands retained their usual characteristics. The changes in cortical macrophages after cyclosporin treatment are discussed, especially in relation to the characteristics of macrophages of the cortico-medullary zone in the normal rat thymus.     

Plastid transformation in greening scales of the onion bulb (Allium cepa,Alliaceae)

The greening of the upper part of the outerAllium cepa L. bulb scales, in particular along the vascular regions, is limited to the hypodermal cells in which typical leucoplasts are transformed to normal and functional chloroplasts. This process is light dependent and cannot afterwards be reversed or modified by darkness. The changes in fine structure are described and briefly discussed.Dedicated to Prof. DrLothar Geitler on the occasion of his 90th birthday and 55 years after the publication of his Grundriß der Cytologie.     

Salt-soluble lectins of corn grain

Lectins extracted from corn (Zea mays L.) kernel with Tris-HCl buffer pH 7.5 were isolated from the crude extract by affinity chromatography on Sepharose 6B-N-acetyl-d-galactosamine and Sepharose 6B-methylα-d-mannoside, and also by lectin affinity chromatography using concanavalin A and Lens culinaris lectin as ligands. According to preferential monosaccharide specificity, salt-soluble lectins of corn seed comprise at least two distinct types: N-acetyl-d-galactosamine-interactive and mannose-interactive lectins. The extracted lectins are unstable, with a tendency to form aggregates during storage.     

Determination of trace elements in standard reference materials by the ko-standardization method

Biological Trace Element Research - The ko-standardization method is suitable for routine multielement determinations by reactor neutron activation analysis (NAA). Investigation of NIST standard...