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排序方式: 共有103条查询结果,搜索用时 15 毫秒
41.
Monocyte chemoattractant protein-1--a major contributor to the inflammatory process associated with diabetes 总被引:2,自引:0,他引:2
There is evidence that strongly suggests that inflammation plays an important role in diabetes and cardiovascular diseases. The high glucose-induced inflammatory process is characterised by the cooperation of a complex network of inflammatory molecules such as cytokines, adhesion molecules, growth factors, and chemokines. Among the chemokine family, monocyte chemoattractant protein (MCP-1) is a potent chemotactic factor, which is upregulated at sites of inflammation being in control of leukocytes trafficking. Here, we review the current knowledge on MCP-1 and its regulation by high glucose level in vascular cells involved in diabetes-induced accelerated atherosclerosis. The signalling pathways involved in MCP-1 modulation by high glucose, the proximal signalling events that stimulate downstream effects and the role of this chemokine in the pathophysiology of diabetes and its complications, are discussed. 相似文献
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Gospodinov A Vaissiere T Krastev DB Legube G Anachkova B Herceg Z 《Molecular and cellular biology》2011,31(23):4735-4745
Chromatin modifications/remodeling are important mechanisms by which cells regulate various functions through providing accessibility to chromatin DNA. Recent studies implicated INO80, a conserved chromatin-remodeling complex, in the process of DNA repair. However, the precise underlying mechanism by which this complex mediates repair in mammalian cells remains enigmatic. Here, we studied the effect of silencing of the Ino80 subunit of the complex on double-strand break repair in mammalian cells. Comet assay and homologous recombination repair reporter system analyses indicated that Ino80 is required for efficient double-strand break repair. Ino80 association with chromatin surrounding double-strand breaks suggested the direct involvement of INO80 in the repair process. Ino80 depletion impaired focal recruitment of 53BP1 but did not impede Rad51 focus formation, suggesting that Ino80 is required for the early steps of repair. Further analysis by using bromodeoxyuridine (BrdU)-labeled single-stranded DNA and replication protein A (RPA) immunofluorescent staining showed that INO80 mediates 5'-3' resection of double-strand break ends. 相似文献
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Kaloyan K. Petrov Dragomir S. Yankov Venko N. Beschkov 《World journal of microbiology & biotechnology》2006,22(4):337-345
Summary The process of lactic acid fermentation of lactose to lactic acid by Lactobacillus rhamnosus ATCC 7469 has been studied. The following processes have been explored: growth kinetics, as well as lactose utilization,
production of lactic acid and further degradation of lactic acid. The immobilization experiments were conducted with microbial
cells entrapped in polyacrylamide gels. Gels with different ratios of the monomer (acrylamide) and the cross-linking agent
(N,N′-methylene-bis-acrylamide) have been tested. These were used in a repeat-batch process. The current processes inside and
outside the gel particles were subjects of examination. The evolution of the activity of immobilized cells with repeated use
showed that the particles served mainly as a donor of cells for the free culture. In all experiments a very high degree of
conversion, 85–90% was observed. After several runs however, the particles were exhausted for microbial cells. A kinetic model
of the process of lactic acid production was developed. This model allowed the evaluation of the effect of microbial growth
and diffusion limitations inside the gel particles on the process rate and the separate contribution of the free and immobilized
cells to the overall fermentation process upon multiple use. 相似文献
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Viara Ivanova Dragomir Yankov Ludmila Kabaivanova Dimitre Pashkoulov 《Microbiological research》2001,156(1):19
Thermostable a-amylase with temperature optimum at 80 °C, molecular mass 58 kDa and pI point 6.9 was purified from a catabolite resistant Bacillus licheniformis strain. The enzyme was sensitive to inhibition by metal ions and N-bromosuccinimide. The partition behaviour of this enzyme in aqueous two-phase systems (ATPS) of the polymer-polymer-water type was investigated and some effects of type, molecular weight and concentration of phase components were studied. Up to 100% retention in the bottom phase of polyethylene glycol 10,000—20,000/dextran 200 system was reached. Best partition conditions were obtained in PEG 10,000—20,000/polyvinyl alcohol 200 systems, where the partition coefficient K increased 750 times to 7.5. Simultaneous production and purification of a-amylase and serine proteinase in PEG-polymer-water ATPS were examined. In the system PEG 6,000/ficoll, up to 90% of the amylase was retained in the bottom phase, whereas about 95% of the total protein (K = 22.8) and 60—75% of the proteinase were in the top phase. Similar separation of the enzymes from laboratory supernatant was obtained in system PEG/Na2SO4. 相似文献
49.
Mihalache D Preoteasa V Carloban A Stănculescu A Dragomir CT 《Roumanian archives of microbiology and immunology》2001,60(4):349-358
Cholesterol plays a critical role not only in modulating membrane structure and dynamics but also in its metabolic pathway, to interfere with cell growth and proliferation processes. Having this aim in mind, we have suggested an investigation, by radioisotopic techniques, of the effect of vitamin E (alpha-tocopherol, 3 mg/kg b.w. in daily doses, for 7 days) on the unscheduled DNA biosynthesis, induced by Romanian cytostatic Lomustin (Nipalkin or CCNU at the dose of 10 mg/kg b.w in acute administration, for 24 h), both on normally fed animals and on rats having a hypercholesterolemic diet, for 30 days. Considering the scientific data from the literature, according to which there is an inverse correlation between the serum cholesterol level and the risk of developing cancer, we tried to investigate the possible influence of Wistar rat cholesterolemic background on the nuclear unscheduled DNA biosynthesis, essential for the conservation of the cell genome integrity. So, it has been noticed that: 1) the DNA lesions induced by the alkylant and tested by the uptake of 3H-Thymidine (200 microCi/100 g b.w.) are reduced after vitamin E treatment, suggesting a protective effect of the antioxidant on the genetic material. 2) on a hypercholesterolemic background the administration of Lomustin produces a decrease of cholesterolemia, suggesting the development of a "facilitating environment" for CCNU action, which appears to confirm the data from the biographical sources. 3) using 3H-Cholesterol (150 microCi/100 g b.w.) to estimate its intracellular liver incorporation suggests a possible displacement of cholesterol from the tissue compartment to the serum one and reverse, event which appears to be correlated with unscheduled DNA biosynthesis. This sustains the idea of the intracellular cholesterol necessity during the nucleic acid biosynthesis as well as in genome aggression. 相似文献
50.
The peptide antibiotic clavanin A interacts strongly and specifically with lipid bilayers 总被引:1,自引:0,他引:1
van Kan EJ Ganchev DN Snel MM Chupin V van der Bent A de Kruijff B 《Biochemistry》2003,42(38):11366-11372
In this study the interaction of the antimicrobial peptide clavanin A with phosphatidylcholine bilayers is investigated by DSC, NMR, and AFM techniques. It is shown that the peptide interacts strongly and specifically with the lipids, resulting in increased order-disorder phase transition temperatures, phase separation, altered acyl chain and headgroup packing, and a drastically changed surface morphology of the bilayer. These results are interpreted in terms of clavanin-specific interactions with lipids and are discussed in the light of the different mechanisms by which clavanin A can destroy the barrier function of biological membranes. 相似文献