排序方式: 共有102条查询结果,搜索用时 15 毫秒
21.
Sándor Bartha Szilárd Szentes András Horváth Judit Házi Zita Zimmermann Csaba Molnár István Dancza Katalin Margóczi Róbert W. Pál Dragica Purger Dávid Schmidt Miklós Óvári Cecília Komoly Zsuzsanna Sutyinszki Gábor Szabó András István Csathó Melinda Juhász Károly Penksza Zsolt Molnár 《应用植被学》2014,17(2):201-213
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Branka Brukner Dabović Dragica Radojković Predrag Minić Jovan Savić Ana Savić 《Human genetics》1992,88(6):699-700
Summary A study was undertaken to find the frequency of the F508 deletion and those of the G551D, R553X and G524X mutations among the mainly Slavic population of Serbia, Bosnia, Herzegovina, and Montenegro and compare the frequencies determined with those in other European populations. The F508 mutation was found to account for about 70% of CF genes in central Jugoslavia, where its frequency is significantly higher than elsewhere in Southern European populations. 相似文献
23.
Chemical Composition and Interpopulation Variability of Essential Oils of Taxus baccata L. from Serbia 下载免费PDF全文
Milena Stefanović Mihailo Ristić Zorica Popović Rada Matić Biljana Nikolić Vera Vidaković Dragica Obratov‐Petković Srdjan Bojović 《化学与生物多样性》2016,13(7):943-953
The composition of the essential oil of the twigs and needles of Taxus baccata L. from three natural populations in Serbia has been determined by GC/MS analysis. Of the 91 detected compounds, 87 were identified. The most abundant compound classes were aliphatic alcohols, terpenes, aliphatic hydrocarbons, and aliphatic aldehydes, which together comprised ca. 86.92% of the total oil composition. The dominant constituents were oct‐1‐en‐3‐ol (23.48%), (3Z)‐hex‐3‐en‐1‐ol (11.46%; aliphatic alcohols), and myrtenol (11.38%; oxygenated monoterpene). The PCA of 22 selected compounds revealed differentiations of populations based on geographic distribution. The CA showed that Populations I and II from the Dinaric Alps were similar, and that Population III from the Balkan mountain system was distinct. This was the first investigation of interpopulation variability of T. baccata populations based on essential oil composition. The results of this study were compared with those of previous studies concerning volatile compounds produced by Taxus species. The results indicate that the essential oil content of T. baccata populations from this study is unique, mostly resembling the population from southeast Serbia. 相似文献
24.
Milos Prokopijevic Olivera Prodanovic Dragica Spasojevic Zeljko Stojanovic Ksenija Radotic Radivoje Prodanovic 《Bioprocess and biosystems engineering》2014,37(5):799-804
Soybean hull peroxidase (SHP, E.C. 1.11.1.7) was immobilized by a glutaraldehyde and periodate method onto series of macroporous copolymers of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA), poly(GMA-co-EGDMA) with various surface characteristics and pore size diameters ranging from 44 to 200 nm. Glutaraldehyde immobilization method and poly(GMA-co-EGDMA) named SGE 20/12 with pore sizes of 120 nm gave immobilized enzyme with highest specific activity of 25 U/g. Deactivation studies showed that immobilization increased stability of SHP and that surface characteristics of the used copolymer had a major influence on a stability of immobilized enzyme at high temperatures and in an organic solvent. The highest thermostability was obtained using the copolymer SGE 20/12 with pore size of 120 nm, while the highest stability in dioxane had SHP immobilized onto copolymer SGE 10/4 with pore size of 44 nm. Immobilized SHP showed a wider pH optimum as compared to the native enzyme especially at alkaline pH values and 3.2 times increased K m value for pyrogallol. After 6 cycles of repeated use in batch reactor, immobilized SHP retained 25 % of its original activity. Macroporous copolymers with different surface characteristics can be used for fine tuning of activity and stability of immobilized SHP to obtain a biocatalyst suitable for phenol oxidation or polymer synthesis in organic solvents. 相似文献
25.
Régis Nouaille Maria Matulova Vladimír Pätoprstý Anne-Marie Delort Evelyne Forano 《Applied microbiology and biotechnology》2009,83(3):425-433
Extracellular culture fluid of Fibrobacter succinogenes S85 grown on glucose, cellobiose, cellulose or wheat straw was analysed by 2D-NMR spectroscopy. Cellodextrins did not accumulate
in the culture medium of cells grown on cellulose or straw. Maltodextrins and maltodextrin-1P were identified in the culture medium of glucose, cellobiose and cellulose grown cells. New glucose derivatives were identified
in the culture fluid under all the substrate conditions. In particular, a compound identified as cellobionic acid accumulated
at high levels in the medium of F. succinogenes S85 cultures. The production of cellobionic acid (and cellobionolactone also identified) was very surprising in an anaerobic
bacterium. The results suggest metabolic shifts when cells were growing on solid substrate cellulose or straw compared to
soluble sugars. 相似文献
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Isabel Mendizabal Oscar Lao Urko M. Marigorta Andreas Wollstein Leonor Gusmão Vladimir Ferak Mihai Ioana Albena Jordanova Radka Kaneva Anastasia Kouvatsi Vaidutis Kučinskas Halyna Makukh Andres Metspalu Mihai G. Netea Rosario de Pablo Horolma Pamjav Dragica Radojkovic Sarah J.H. Rolleston Jadranka Sertic Milan Macek Manfred Kayser 《Current biology : CB》2012,22(24):2342-2349
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Zendelovska D Simeska S Sibinovska O Kostova E Milosevska K Jakovski K Jovanovska E Kikerkov I Trojacanec J Zafirov D 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2006,839(1-2):85-88
Nifedipine, a dihydropyridine calcium channel antagonist, is widely used in the treatment of hypertension and other cardiovascular disorders. A selective, sensitive and accurate high-performance liquid chromatographic method has been developed, validated and applied for determination of nifedipine in human plasma samples. A series of studies were conducted in order to investigate the effects of mobile phase composition, buffer concentration, mobile phase pH and concentration of organic modifiers, and to develop a convenient and easy-to-use method for quantitative analysis of nifedipine. The method involves solid-phase extraction on C18 cartridges. The chromatographic separation was accomplished on a Lichrocart Lichrospher 60 RP selectB column with a mobile phase composed of 0.020 mol/L KH2PO4 (pH 4.8) and acetonitrile (42:58, v/v). UV detection was set at 240 nm. The calibration curve was linear in the concentration range of 5.0-200.0 ng/mL for nifedipine in plasma and the limit of quantification was 5.0 ng/mL. 相似文献
29.
We show for the first time the occurrence of maltodextrin-1-Phosphate (MD-1P) (DP2) in F. succinogenes S85, a rumen bacterium specialized in cellulolysis which is not able to use maltose and starch. MD-1P were found in intra and extracellular medium of resting cells incubated with glucose. We used 2D 1H NMR technique and TLC to identify their structure and quantify their production with time. It was also shown that these phosphorylated oligosaccharides originated both from exogenous glucose and endogenous glycogen. 相似文献
30.
Platelet serotonin concentration in alcoholic subjects 总被引:1,自引:0,他引:1
Pivac N Mück-Seler D Mustapić M Nenadić-Sviglin K Kozarić-Kovacić D 《Life sciences》2004,76(5):521-531
Serotonin (5-hydroxytryptamine, 5-HT) is assumed to play a role in the pathophysiology of different psychiatric disorders including alcoholism. Since platelets and central serotonergic synaptosomes share similar pharmacodynamics of 5-HT, this study determined platelet 5-HT concentration in 148 male and 42 female drug-free subjects with alcohol dependency, according to the DSM-IV criteria, and in sex-and age-matched controls. Male and female alcoholics had significantly lower platelet 5-HT concentration than 110 male and 123 female healthy controls. Sex differences, i.e. higher platelet 5-HT concentration in men than in women, were found both in healthy and alcoholic subjects. Platelet 5-HT concentration differed significantly in male and female alcoholic subjects with or without different psychiatric comorbidities. Platelet 5-HT concentration was higher in male alcoholics with comorbid posttraumatic stress disorder (PTSD) than in male alcoholics with comorbid anxious-depressive disorder, or depression, or male alcoholics without any psychiatric comorbidities. Comorbid depression in female alcoholics slightly elevated platelet 5-HT levels but these values were still reduced compared to values in healthy women. Smoking status did not affect platelet 5-HT concentration either in healthy or in alcoholic subjects. The data from our study show sex differences, and reduced platelet 5-HT values, regardless of the nicotine dependence, in the large groups of male and female alcoholic subjects. Among male alcoholics the presence of comorbid PTSD partly normalized the decreased platelet 5-HT values. The results of the present study support the hypothesis that alterations in 5-HT system might be related to alcoholism. 相似文献