Differences in the methylation of brain histamine in vivo between audiogenic seizure-sensitive and -resistant deermice

We have investigated the catabolism of [³H] histamine (HA), after intraventricular (i.vt.) administration, in brains of the audiogenic seizure susceptible (SS) and resistant (SR) deermouse $\text{Peromyscus}$. Brains of SS mice had lower endogenous HA levels and contained less [³H]-HA 20, 60 and 300 sec after i.vt. [³H]-HA than did brains of SR deermice. Twenty sec after [³H]-HA, brain [³H] methylhistamine (MeHA) levels and the resulting MeHA conversion index were found to be increased in the SS animals while later, at 60 and 300 sec, these parameters were found to be decreased. There were no SS-SR differences in the levels of brain [³H] methylimidazoleacetic acid. The data indicate that SS deermice catabolize exogenous HA, at least initially, more rapidly than their SR counterparts, confirming a like result noted immediately prior to seizure activity elicited by the administration of L-methionine-dl-sulfoximine in $\text{Mus}$.     

l-3-(3-carboxyfuran-4-yl)alanine,a new amino acid from the mushroom Phyllotopsis nidulans

A new amino acid has been discovered in uncombined form in extracts of the fruiting bodies of the mushroom, Phyllotopsis nidulans. Chemical and spectroscopic data support formulation of the structure as l-3-(3-carboxyfuran-4-yl)alanine.     

The role of surface stress in the morphology of microbes

The shapes of many prokaryotes can be understood by the assumption that the cell wall expands in response to tension created by the osmotically derived hydrostatic pressure. Different organisms have different shapes because wall growth takes place in different regions. A previous paper (Koch et al., 1981 a) considered the simplest case of prokaryotic growth, i.e. that of Streptococcus faecium. In the present paper, an elaboration of this theory is applied to two further cases - the more perfectly spherical cocci and the rod-shaped bacteria. These cases are more complex mathematically, because growth over a considerable fraction of the surface must be considered. Such diffuse growth cannot be treated analytically, but can be simulated on a computer or handled by geometric arguments. The spherical form of the cocci may result from either diffuse growth over their entire external surface, or from zonal growth in which the addition of new material only occurs in the immediate vicinity of the splitting septum. In the zonal model, it must be assumed that the least amount of previously laid down septal peptidoglycan consistent with wall growth is reworked in the formation of the new external wall. For Gram-positive rods, where the body of the rod is truly cylindrical, three kinds of growth zones are required: (1) the inward edge of the ingrowing septum, (2) the junction of septum and nascent pole, and (3) the cylindrical walls. Two modes for cylindrical elongation ara possible: (a) new wall is added in one or a few narrow annular zones, or (b) new wall material is added continuously all over the innermost surface and the outer layer is degraded. It is shown that the latter case applies to Bacillus subtilis. Also summarized in this paper are results, developed in more detail elsewhere, concerning the morphology of fusiform bacteria, Gram-negative rods and the hyphal tips of fungi.     

A comparative study of the phytochemistry of two African Rain Forests

A comparative analysis of aspects of the secondary chemistry of plants from the Kibale Forest, Uganda, and the Douala-Edea Forest Reserve, Cameroon (93 species in all) has shown mean concentrations of tannins and other phenolics to be significantly greater in both young and mature foliage from Douala-Edea than in comparable taxa from Kibale. The differences remain significant when analysis was restricted to common species only. Chromatographic analysis confirmed that the proportion of species from Douala-Edea yielding tannin breakdown products was significantly greater than that from Kibale. In contrast, the proportion of species whose leaves gave definite alkaloid-positive Dragendorff reactions was significantly higher in the Kibale site. Within each site, mature leaves of the great majority of species yielded either tannins or alkaloids but the presence of detectable quantities of both types of compound was rare. In each site distributions of the two classes relative to one another departed significantly from independence at the O < 0.005 level. Nutrient analyses of vegetation also showed striking differences between the two sites; the Ugandan material appearing to be more nutrient-rich. The distribution patterns of nutrients and of secondary metabolites between the two sites are discussed in relation to current hypotheses concerning strategies of defence chemistry among plant communities. The data suggest that in Douala-Edea, which is characterized by very sandy and acid soils, the common elements of the flora invest heavily in the production of high concentrations of tannins and other phenolics. According to current hypotheses, these are the class of secondary compounds whose characteristics are most suitable to defence of vegetation growing on poor soils, and/or in species-poor stands, and in which leaves are likely to be long-lived.     

A foetal alpha-1 antitrypsin which resembles PI*I

Summary A transient form of alpha-1 antitrypsin (PI) with an isoelectric point resembling that of the inherited PI*I allele has been detected in ten premature infants up to 35/40 weeks' gestation. It is suggested that this may be a foetal form of alpha-1 antitrypsin which is specially adapted to the uterine evironment.     

Demonstration of shared antigenic determinants between Streptococcus mutans BHT cell membrane, human heart tissue and myosin using monoclonal antibodies to S. mutans

Monoclonal antibodies (MAb) raised to intact Streptococcus mutans P-4 cells (serotype e) were used to demonstrate the presence of shared antigenic determinant(s) between S. mutans BHT (serotype b) cell membranes and human heart tissue. MAb binding to both BHT membrane and human heart tissue was demonstrated by ELISA. Common antigens were identified by immunoblot analysis following separation of BHT membrane components and human heart antigens by SDS-PAGE. MAb 22C4 recognized three polypeptides from the BHT membrane preparation, having molecular masses of 42, 56 and 85 kDa. MAb 22C4 also recognized an 85 kDa component and a 200 kDa component from human heart tissue. MAb D159 was specific for a single 82 kDa polypeptide in BHT membrane, and also bound to two high molecular mass components in human heart (165 and 200 kDa). When both MAb D159 and 22C4 were first absorbed with S. mutans P-4 cells, subsequent reactivity to the aforementioned BHT membrane components was inhibited, indicating that these cross-reactive components are found in S. mutans P-4 as well as in S. mutans BHT micro-organisms. Competitive binding analysis showed that both MAb D159 and MAb 22C4 bound to myosin, indicating that S. mutans BHT membrane, human heart tissue and myosin share at least one immunodeterminant. This indicates that myosin could be the cross-reactive tissue component in human heart.     

Cytotoxic activity ofEscherichia coli O157:H7 culture filtrate on the mouse colon and kidney

Escherichia coli O157:H7 culture filtrate (O157CF) produced colonic and renal lesions in mice following intraperitoneal or intravenous injection. Colonic lesions were characterized by death and sloughing of both surface and crypt epithelial cells, leading to loss of the mucous membrane and subsequent occult colonic hemorrhage. Several areas of severe colonic damage existed where loss of the epithelium and lamina propria was complete, leaving only the submucosal and smooth muscle layers intact. The colon was the only portion of the gastrointestinal tract affected by O157CF. Renal lesions were characterized by marked vacuolation and general necrosis of proximal convoluted tubular cells, and the presence of numerous exfoliated renal epithelial cells in the lumina of distal convoluted and collecting tubules. A neurogenic response was demonstrated by paralysis of the animals' rear extremities. The mouse was a useful model for detecting and studying in vivo the toxic properties of O157CF.