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91.
Kirsten Krastel Dilani B. Senadheera Richard Mair Jennifer S. Downey Steven D. Goodman Dennis G. Cvitkovitch 《Journal of bacteriology》2010,192(4):984-993
Glutamate contributes to the acid tolerance response (ATR) of many Gram-negative and Gram-positive bacteria, but its role in the ATR of the oral bacterium Streptococcus mutans is unknown. This study describes the discovery and characterization of a glutamate transporter operon designated glnQHMP (Smu.1519 to Smu.1522) and investigates its potential role in acid tolerance. Deletion of glnQHMP resulted in a 95% reduction in transport of radiolabeled glutamate compared to the wild-type UA159 strain. The addition of glutamate to metabolizing UA159 cells resulted in an increased production of acidic end products, whereas the glnQHMP mutant produced less lactic acid than UA159, suggesting a link between glutamate metabolism and acid production and possible acid tolerance. To investigate this possibility, we conducted a microarray analysis with glutamate and under pH 5.5 and pH 7.5 conditions which showed that expression of the glnQHMP operon was downregulated by both glutamate and mild acid. We also measured the growth kinetics of UA159 and its glnQHMP-negative derivative at pH 5.5 and found that the mutant doubled at a much slower rate than the parent strain but survived at pH 3.5 significantly better than the wild type. Taken together, these findings support the involvement of the glutamate transporter operon glnQHMP in the acid tolerance response in S. mutans.Streptococcus mutans is 1 of over 700 bacterial species commonly found in the oral environment (1). Its ability to rapidly metabolize dietary carbohydrates to acid end products causes demineralization of the tooth enamel, leading to caries formation (19). Acidogenicity (the ability to produce acid end products via glycolysis) and aciduricity (the ability to survive and grow in acidic environments) are two important virulence factors of S. mutans. Maintenance of a pH gradient across the cell membrane by increasing intracellular pH by 0.5 to 1.0 relative to the extracellular pH (ΔpH) when exposed to a low pH environment is critical for the survival of S. mutans at low pH. This is primarily accomplished by acid-induced mechanisms that facilitate proton extrusion via the proton-translocating ATPase (5, 20) and by acid end product efflux (8, 12). S. mutans also possesses an acid tolerance response (ATR) mechanism, whereby preexposure to sublethal pH environments (e.g., pH 5.5) affords protection from killing under lethal pH values as low as pH 3.0 (7). This adaptive process is characterized by increased acid resistance (4), increased glycolytic capacities (20), and increased proton-translocating enzyme F1F0-ATPase activity (44). The ATR is enhanced by sugar starvation and the addition of amino acids (48), the addition of potassium ions (12), growth in biofilms, and activity of multiple two-component signal transduction systems that include the ComDE, HK11/RR11 (also designated LiaS/LiaR), VicKR, CiaHR, LevSR, ScnKR, and HK1037/RR1038 (6, 17, 31, 32, 46).Previously, Noji et al. and Sato et al. described a glutamate/aspartate transporter in S. mutans (38, 45). Those researchers showed that the presence of potassium ions was required for transport and that, in environments of pH 6.0 or below, the activity of the H+-ATPase system was required (38, 45). Potassium ions are the main cations in plaque (50), and potassium uptake is associated with intracellular pH homeostasis in S. mutans (24, 35). In addition, expression of several genes involved in the glutamate synthesis pathway (icd, citZ, and acn) are downregulated under low pH (10), suggesting a link between glutamate metabolism, potassium levels, and aciduricity in S. mutans. Since acid tolerance is an important virulence property of S. mutans, we aimed to investigate a possible link between glutamate uptake and acid resistance in this oral pathogen. In bacteria, intracellular glutamate and glutamine levels are closely linked with nitrogen metabolism of the cell. Glutamine is synthesized from glutamate and ammonium, which is a major way for cells to assimilate the nitrogen required for biosynthesis of all amino acids, thus affecting protein synthesis and the structural and functional integrity of the cell. Notably, nitrogen metabolism, especially glutamine metabolism, has been linked to virulence in a number of microorganisms, including Streptococcus pneumoniae (26, 42), Staphylococcus aureus (41), Candida albicans (33), and Pseudomonas aeruginosa (51). Glutamate uptake and metabolism are known to be involved in the ATR of Gram-negative bacteria such as Escherichia coli via the use of glutamate decarboxylase and the glutamate/gamma-amino butyrate (glutamate/GABA) antiporter (9). Similarly, the homologous proteins of these systems in Lactococcus lactis, encoded by the gadBC genes, were shown to assist in a glutamate-dependent acid-resistance mechanism in that Gram-positive bacterium (44).In this study, we searched the S. mutans UA159 genome for potential glutamine transporter operons. We constructed a deletion mutant (SmuGLT) of the glnQHMP operon (Smu.1519 to Smu.1522) and confirmed its role as a glutamate transporter. The inability of SmuGLT to take up glutamate resulted in a general growth deficiency, especially at pH 5.5, as well as an increased tolerance to acid. Results from this study provide insight into the ATR of S. mutans, including a potential link between glutamate metabolism and acid resistance in S. mutans. 相似文献
92.
Ryou MG Sun J Oguayo KN Manukhina EB Downey HF Mallet RT 《Experimental biology and medicine (Maywood, N.J.)》2008,233(6):766-774
Physiologically modulated concentrations of nitric oxide (NO) are generally beneficial, but excessive NO can injure myocardium by producing cytotoxic peroxynitrite. Recently we reported that intermittent, normobaric hypoxia conditioning (IHC) produced robust cardioprotection against infarction and lethal arrhythmias in a canine model of coronary occlusion-reperfusion. This study tested the hypothesis that IHC suppresses myocardial nitric oxide synthase (NOS) activity and thereby dampens explosive, excessive NO formation upon reperfusion of occluded coronary arteries. Mongrel dogs were conditioned by a 20 d program of IHC (FIO(2) 9.5-10%; 5-10 min hypoxia/cycle, 5-8 cycles/d with intervening 4 min normoxia). One day later, ventricular myocardium was sampled for NOS activity assays, and immunoblot detection of the endothelial NOS isoform (eNOS). In separate experiments, myocardial nitrite (NO(2)(-)) release, an index of NO formation, was measured at baseline and during reperfusion following 1 h occlusion of the left anterior descending coronary artery (LAD). Values in IHC dogs were compared with respective values in non-conditioned, control dogs. IHC lowered left and right ventricular NOS activities by 60%, from 100-115 to 40-45 mU/g protein (P < 0.01), and decreased eNOS content by 30% (P < 0.05). IHC dampened cumulative NO(2)(-) release during the first 5 min reperfusion from 32 +/- 7 to 14 +/- 2 mumol/g (P < 0.05), but did not alter hyperemic LAD flow (15 +/- 2 vs. 13 +/- 2 ml/g). Thus, IHC suppressed myocardial NOS activity, eNOS content, and excessive NO formation upon reperfusion without compromising reactive hyperemia. Attenuation of the NOS/NO system may contribute to IHC-induced protection of myocardium from ischemia-reperfusion injury. 相似文献
93.
94.
Abattoir-derived oocytes were exposed to a concentrated cryoprotectant solution (DAP213: 2 M DMSO, 1 M acetamide, 3 M propanediol, and 10% FCS in TCM199) for 1.5 or 5 min at the germinal vesicle (GV) stage or after maturation in vitro (IVM). Their viability was assessed by in vitro fertilization (IVF) and culture (IVC) to blastocysts. To investigate the effect of DAP213 on the ultrastructure, GV and IVM oocytes were processed for transmission electron microscopy (TEM) before (control) or after exposure to the cryoprotectant. DAP213 induced profound ultrastructural modifications to the microvilli and mitochondria, resulted in large vesicle formation, and, most significantly, caused the premature release of the cortical granules (CG). In IVM oocytes exposed to the cryoproteclant for 5 min, exocytosis of CG into the perivitelline space was common and the IVF rate was reduced (P <.05). After exposure for 5 min, GV oocytes displayed clusters of CG comparable to controls, but after IVM-IVF, polyspermy rate was increased (P <.05). Furthermore, treated GV oocytes showed a reduced rate of cleavage and blastocyst formation and an increased percentage of oocytes exhibiting alterations in organelles, whereas the viability and ultrastructure of IVM oocytes treated for 1.5 min was not different from controls. These observations demonstrate that (1) cortical granule kinetics is one of the key elements controlling fertilizability of bovine oocytes treated with cryoprotectant, and (2) GV oocytes are more sensitive to the cryoprotectant than those that have already been matured in vitro. 相似文献
95.
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97.
Biophysical properties and microfilament assembly in neutrophils: modulation by cyclic AMP 下载免费PDF全文
G P Downey E L Elson B Schwab S C Erzurum S K Young G S Worthen 《The Journal of cell biology》1991,114(6):1179-1190
The microfilament lattice, composed primarily of filamentous (F)-actin, determines in large part the mechanical (deformability) properties of neutrophils, and thus may regulate the ability of neutrophils to transit a microvascular bed. Circulating factors may stimulate the neutrophil to become rigid and therefore be retained in the capillaries. We hypothesized that cell stiffening might be attenuated by an increase in intracellular cAMP. A combination of cell filtration and cell poking (mechanical indentation) was used to measure cell deformability. Neutrophils pretreated with dibutyryl cAMP (db-cAMP) or the combination of prostaglandin E2 (PGE2, a stimulator of adenylate cyclase) and isobutylmethylxanthine (IBMX, an inhibitor of phosphodiesterase) demonstrated significant inhibition of the n-formyl-methionyl-leucyl-phenylalanine (fMLP)-inducing stiffening. The inhibition of cell stiffening was associated with an increase in intracellular cAMP as measured by enzyme-linked immunoassay (EIA) and an increase in the activity of the cAMP-dependent kinase (A-kinase). Treatment with PGE2 and IBMX also resulted in a decrease in the F-actin content of stimulated neutrophils as assayed by NBD-phallacidin staining and flow cytometry or by changes in right angle light scattering. Direct addition of cAMP to electropermeabilized neutrophils resulted in attenuation of fMLP-induced actin assembly. Neutrophils stimulated with fMLP demonstrated a rapid redistribution of F-actin from a diffuse cortical location to a peripheral ring as assessed by conventional and scanning confocal fluorescence microscopy. Pretreatment of neutrophils with the combination of IBMX and PGE2 resulted in incomplete development and fragmentation of the cortical ring. We conclude that assembly and redistribution of F-actin may be responsible for cell stiffening after exposure to stimulants and that this response was attenuated by agents that increase intracellular cAMP, by altering the amount and spatial organization of the microfilament component of the cytoskeleton. 相似文献
98.
Owen M. Wilkins Sondra L. Downey Emily S. Weyburne David A. Williams Anne C. Mirabella Herman S. Overkleeft Alexei F. Kisselev 《Analytical biochemistry》2014
Proteasome-Glo is a homogeneous cell-based assay of proteasomal chymotrypsin-like, trypsin-like, and caspase-like activities using luminogenic substrates, commercially available from Promega. Here we report that the background activity from cleavage of the substrate of the trypsin-like sites by nonproteasomal proteases in multiple breast and lung cancer cell lines exceeds the activity of the proteasome. We also observed substantial background chymotrypsin-like activity in some cell lines. Thus, Proteasome-Glo assay must be used with caution, and it is necessary to include a specific proteasome inhibitor to determine the background for each proteasome activity. 相似文献
99.
A new locus for autosomal dominant familial exudative vitreoretinopathy maps to chromosome 11p12-13 下载免费PDF全文
Downey LM Keen TJ Roberts E Mansfield DC Bamashmus M Inglehearn CF 《American journal of human genetics》2001,68(3):778-781
We report a new locus for familial exudative vitreoretinopathy (FEVR), on chromosome 11p12-13 in a large autosomal dominant pedigree. Statistically significant linkage was achieved across a 14-cM interval flanked by markers GATA34E08 and D11S4102, with a maximum multipoint LOD score of 6.6 at D11S2010. FEVR is a disease characterized by the failure of development of peripheral retinal blood vessels, and it is difficult to diagnose clinically because of the wide spectrum of fundus abnormalities associated with it. The identification of a new locus is important for genetic counseling and potentiates further studies aimed toward the identification of a gene with an important role in angiogenesis within neuroepithelial tissues. Such a gene may also have a role in the genetic predisposition to retinopathy of prematurity, a sporadic disorder with many clinical similarities to FEVR. 相似文献
100.
Actin assembly in electropermeabilized neutrophils: role of intracellular calcium 总被引:6,自引:2,他引:6 下载免费PDF全文
Assembly of microfilaments involves the conversion of actin from the monomeric (G) to the filamentous (F) form. The exact sequence of events responsible for this conversion is yet to be defined and, in particular, the role of calcium remains unclear. Intact and electropermeabilized human neutrophils were used to assess more directly the role of cytosolic calcium [( Ca2+]i) in actin assembly. Staining with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin and right angle light scattering were used to monitor the formation of F-actin. Though addition of Ca2+ ionophores can be known to induce actin assembly, the following observations suggest that an increased [Ca2+]i is not directly responsible for receptor-induced actin polymerization: (a) intact cells in Ca2(+)-free medium, depleted of internal Ca2+ by addition of ionophore, responded to the formyl peptide fMLP with actin assembly despite the absence of changes in [Ca2+]i, assessed with Indo-1; (b) fMLP induced a significant increase in F-actin content in permeabilized cells equilibrated with medium containing 0.1 microM free Ca2+, buffered with up to 10 mM EGTA; (c) increasing [Ca2+]i beyond the resting level by direct addition of CaCl2 to permeabilized cells resulted in actin disassembly. Conversely, lowering [Ca2+]i resulted in spontaneous actin assembly. To reconcile these findings with the actin-polymerizing effects of Ca2+ ionophores, we investigated whether A23187 and ionomycin induced actin assembly by a mechanism independent of, or secondary to the increase in [Ca2+]i. We found that the ionophore-induced actin assembly was completely inhibited by the leukotriene B4 (LTB4) antagonist LY-223982, implying that the ionophore effect was secondary to LTB4 formation, possibly by stimulation of phospholipase A2. We conclude that actin assembly is not mediated by an increase in [Ca2+]i, but rather that elevated [Ca2+]i facilitates actin disassembly, an effect possibly mediated by Ca2(+)-sensitive actin filament-severing proteins such as gelsolin. Sequential actin assembly and disassembly may be necessary for functions such as chemotaxis. 相似文献