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51.
Clete S. Dorsey 《The Western journal of medicine》1962,96(6):412-413
A number of plants indigenous to California can cause contact dermatitis similar to that caused by poison oak—philodendron, oleander, fig family, castor bean, chrysanthemum family, Chinese rice paper plant and several others.Patch testing can be used to prove a suspected diagnosis.In some occupations dermatitis from one or another of these agents is more or less common. 相似文献
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P Saá GF Sferrazza G Ottenberg AM Oelschlegel K Dorsey CI Lasmézas 《Journal of virology》2012,86(19):10494-10504
Several lines of evidence suggest that various cofactors may be required for prion replication. PrP binds to polyanions, and RNAs were shown to promote the conversion of PrP(C) into PrP(Sc) in vitro. In the present study, we investigated strain-specific differences in RNA requirement during in vitro conversion and the potential role of RNA as a strain-specifying component of infectious prions. We found that RNase treatment impairs PrP(Sc)-converting activity of 9 murine prion strains by protein misfolding cyclic amplification (PMCA) in a strain-specific fashion. While the addition of RNA restored PMCA conversion efficiency, the effect of synthetic polynucleotides or DNA was strain dependent, showing a different promiscuity of prion strains in cofactor utilization. The biological properties of RML propagated by PMCA under RNA-depleted conditions were compared to those of brain-derived and PMCA material generated in the presence of RNA. Inoculation of RNA-depleted RML in Tga20 mice resulted in an increased incidence of a distinctive disease phenotype characterized by forelimb paresis. However, this abnormal phenotype was not conserved in wild-type mice or upon secondary transmission. Immunohistochemical and cell panel assay analyses of mouse brains did not reveal significant differences between mice injected with the different RML inocula. We conclude that replication under RNA-depleted conditions did not modify RML prion strain properties. Our study cannot, however, exclude small variations of RML properties that would explain the abnormal clinical phenotype observed. We hypothesize that RNA molecules may act as catalysts of prion replication and that variable capacities of distinct prion strains to utilize different cofactors may explain strain-specific dependency upon RNA. 相似文献
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Background
Clearance of synaptically released glutamate, and hence termination of glutamatergic neurotransmission, is carried out by glutamate transporters, most especially glutamate transporter-1 (GLT-1) and the glutamate-aspartate transporter (GLAST) that are located in astrocytes. It is becoming increasingly well appreciated that changes in the function and expression of GLT-1 and GLAST occur under different physiological and pathological conditions. Here we investigated the plasticity in expression of GLT-1 and GLAST in the spinal dorsal horn using immunohistochemistry following partial sciatic nerve ligation (PSNL) in rats.Results
Animals were confirmed to develop hypersensitivity to mechanical stimulation by 7 days following PSNL. Baseline expression of GLT-1 and GLAST in naive animals was only observed in astrocytes and not in either microglia or neurons. Microglia and astrocytes showed evidence of reactivity to the nerve injury when assessed at 7 and 14 days following PSNL evidenced by increased expression of OX-42 and GFAP, respectively. In contrast, the total level of GLT-1 and GLAST protein decreased at both 7 and 14 days after PSNL. Importantly, the cellular location of GLT-1 and GLAST was also altered in response to nerve injury. Whereas activated astrocytes showed a marked decrease in expression of GLT-1 and GLAST, activated microglia showed de novo expression of GLT-1 and GLAST at 7 days after PSNL and this was maintained through day 14. Neurons showed no expression of GLT-1 or GLAST at any time point.Conclusion
These results indicate that the expression of glutamate transporters in astrocytes and microglia are differentially regulated following nerve injury. 相似文献56.
Sheila Dorsey Vinton Linda Perry Karl J. Reinhard Calogero M. Santoro Isabel Teixeira-Santos 《PloS one》2009,4(11)
The impact of expanding civilization on the health of American indigenous societies has long been studied. Most studies have focused on infections and malnutrition that occurred when less complex societies were incorporated into more complex civilizations. The details of dietary change, however, have rarely been explored. Using the analysis of starch residues recovered from coprolites, here we evaluate the dietary adaptations of indigenous farmers in northern Chile''s Atacama Desert during the time that the Inka Empire incorporated these communities into their economic system. This system has been described as “complementarity” because it involves interaction and trade in goods produced at different Andean elevations. We find that as local farming societies adapted to this new asymmetric system, a portion of their labor had to be given up to the Inka elite through a corvée tax system for maize production. In return, the Inka system of complementarity introduced previously rare foods from the Andean highlands into local economies. These changes caused a disruption of traditional communities as they instituted a state-level economic system on local farmers. Combined with previously published infection information for the same populations under Inka rule, the data suggest that there may have been a dual health impact from disruption of nutrition and introduction of crowd disease. 相似文献
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Dorsey DA Mascó DH Dikranian K Hyrc K Masciotra L Faddis B Soriano M Gru AA Goldberg MP de Erausquin GA 《Apoptosis : an international journal on programmed cell death》2006,11(4):535-544
Developing neuronal populations undergo significant attrition by natural cell death. Dopaminergic neurons in the substantia
nigra pars compacta undergo apoptosis during synaptogenesis. Following this time window, destruction of the anatomic target
of dopaminergic neurons results in dopaminergic cell death but the morphology is no longer apoptotic. We describe ultrastructural
changes that appear unique to dying embryonic dopaminergic neurons. In primary cultures of mesencephalon, death of dopaminergic
neurons is triggered by activation of glutamate receptors sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic
acid (AMPA), and differs ultrastructurally from both neuronal apoptosis or typical excitotoxicity. AMPA causes morphological
changes selectively in dopaminergic neurons, without affecting other neurons in the same culture dishes. Two hours after the
onset of treatment swelling of Golgi complexes is apparent. At 3 h, dopaminergic neurons display loss of membrane asymmetry
(coinciding with commitment to die), as well as nuclear membrane invagination, irregular aggregation of chromatin, and mitochondrial
swelling. Nuclear changes continue to worsen until loss of cytoplasmic structures and cell death begins to occur after 12 h.
These changes are different from those described in neurons undergoing either apoptosis or excitotoxic death, but are similar
to ultrastructural changes observed in spontaneous death of dopaminergic neurons in the natural mutant weaver mouse. 相似文献
60.
This protein assay requires heating the sample with biuret reagent for 100 min at 100°C before addition of Folin. Different proteins give almost identical optical densities on a nitrogen basis with this procedure. The absorbance per microgram-atom of protein nitrogin is linear at or below 0.400. A convenient final concentration of protein in this assay is about 0.2 μg-atom of protein nitrogen per ml. Most of the absorbance is due to amino acid combinations other than tyrosine and trytophan in the protein. The specificity appears similar to that of the bluret where few compounds of biological importance interfere significantly in the assay at normal cellular concentrations. 相似文献