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991.
992.
Sułek A Hoffman-Zacharska D Bednarska-Makaruk M Szirkowiec W Zaremba J 《Journal of applied genetics》2004,45(1):101-105
Spinocerebellar ataxias are a group of neurodegenerative disorders caused by dynamic mutations of microsatellite repeats. Two novel forms of SCAs have been described recently: SCA8, with expansions of CTA/CTG repeats in 3'UTR of the SCA8 gene, and SCA12, caused by expansion of the CAG tract in 5'UTR of the SCA12/PP2R2B gene. Analysis of CTA/CTG and CAG polymorphism in those two genes was performed in a Polish control group consisting of 100 individuals without any neurological signs. The distribution and ranges of the number of non-pathogenic repeats were similar to those observed in other populations described previously. Expansion of CTA/CTG repeats in the SCA8 locus was found in 2 of 100 controls and in 5 probands among 150 pedigrees affected with unidentified ataxias. As such expanded alleles were also observed in their healthy relatives, the pathogenic role of expansions in the SCA8 gene remains uncertain. 相似文献
993.
994.
Dorota Piekutowska-Abramczuk Zahra Assouline Lavinija Mataković René G. Feichtinger Eliška Koňařiková Elżbieta Jurkiewicz Piotr Stawiński Mirjana Gusic Andreas Koller Agnieszka Pollak Piotr Gasperowicz Joanna Trubicka Elżbieta Ciara Katarzyna Iwanicka-Pronicka Dariusz Rokicki Sylvain Hanein Saskia B. Wortmann Wolfgang Sperl Johannes A. Mayr 《American journal of human genetics》2018,102(3):460-467
995.
Multiple phosphorylation sites on γ‐tubulin are essential and contribute to the biogenesis of basal bodies in Tetrahymena 下载免费PDF全文
996.
Katarzyna Pańczyk Karolina Pytka Magdalena Jakubczyk Anna Rapacz Kinga Sałat Anna Furgała Agata Siwek Monika Głuch-Lutwin Anna Gryboś Karolina Słoczyńska Elżbieta Pękala Paweł Żmudzki Adam Bucki Marcin Kołaczkowski Dorota Żelaszczyk Henryk Marona Anna M. Waszkielewicz 《Bioorganic & medicinal chemistry letters》2018,28(11):2039-2049
Aim of the study was evaluation of anxiolytic, antidepressant, anticonvulsant and analgesic activity in a series of a consistent group of compounds. A series of eleven new N-(phenoxyalkyl)- or N-{2-[2-(phenoxy)ethoxy]ethyl}piperazine derivatives has been obtained. Their affinity towards 5-HT1A, 5-HT2A, 5-HT6, 5-HT7, D2 and α1 receptors has been assessed, and then functional assays were performed. The compounds were evaluated in mice, i.p. for their antidepressant-like (forced swim test), locomotor, anxiolytic-like (four-plate test) activities as well as – at higher doses – for anticonvulsant potential (MES) and neurotoxicity (rotarod). Two compounds (3, 6) were also evaluated for their analgesic activity in neuropathic pain models (streptozocin test, oxaliplatin test) and they were found active against allodynia in diabetic neuropathic pain at 30?mg/kg. Among the compounds, anxiolytic-like, anticonvulsant or analgesic activity was observed but antidepressant-like activity was not. One of the two most interesting compounds is 1-{2-[2-(2,4,6-trimethylphenoxy)ethoxy]ethyl}-4-(2-methoxyphenyl)piperazine dihydrochloride (9), exhibiting anxiolytic and anticonvulsant activity in mice, i.p. 30 min after administration (at 2.5?mg/kg and ED50?=?26.33?mg/kg, respectively), which can be justified by the receptor profile: 5-HT1A Ki?=?5?nM (antagonist), 5-HT7 Ki?=?70?nM, α1 Ki?=?15?nM, D2 Ki?=?189?nM (antagonist). Another interesting compound is 1-[3-(2,4,6-trimethylphenoxy)propyl]-4-(4-methoxyphenyl)piperazine dihydrochloride (3), exhibiting anxiolytic, anticonvulsant and antiallodynic activity in mice, i.p., 30?min after administration (at 10?mg/kg, ED50?=?23.50?mg/kg, at 30?mg/kg, respectively), which can be related with 5-HT1A weak antagonism (Ki?=?146?nM), or other possible mechanism of action, not evaluated within presented study. Additionally, for the most active compound in the four-plate test (7), molecular modeling was performed (docking to receptors 5-HT1A, 5-HT2A, 5-HT7, D2 and α1A). 相似文献
997.
998.
Lidia Lipińska Robert Klewicki Michał Sójka Radosław Bonikowski Dorota Żyżelewicz Krzysztof Kołodziejczyk Elżbieta Klewicka 《Probiotics and antimicrobial proteins》2018,10(2):186-200
The antifungal activity of Lactobacillus pentosus ?OCK 0979 depends both on the culture medium and on the fungal species. In the control medium, the strain exhibited limited antagonistic activity against indicator food-borne molds and yeasts. However, the supplementation of the bacterial culture medium with polyols (erythritol, lactitol, maltitol, mannitol, sorbitol, xylitol) or their galactosyl derivatives (gal-erythritol, gal-sorbitol, gal-xylitol) enhanced the antifungal properties of Lactobacillus pentosus ?OCK 0979. Its metabolites were identified and quantified by enzymatic methods, HPLC, UHPLC-MS coupled with QuEChERS, and GC-MS. The presence of polyols and gal-polyols significantly affected the acid metabolite profile of the bacterial culture supernatant. In addition, lactitol and mannitol were used by bacteria as alternative carbon sources. A number of compounds with potential antifungal properties were identified, such as phenyllactic acid, hydroxyphenyllactic acid, and benzoic acid. Lactobacillus bacteria cultivated with mannitol synthesized hydroxy-fatty acids, including 2-hydroxy-4-methylpentanoic acid, a well-described antifungal agent. Scanning electron microscopy (SEM) and light microscopy confirmed a strong antifungal effect of L. pentosus ?OCK 0979. 相似文献
999.
Jakub Nowosad Alfred Stach Idalia Kasprzyk Kazimiera Chłopek Katarzyna Dąbrowska-Zapart Łukasz Grewling Małgorzata Latałowa Anna Pędziszewska Barbara Majkowska-Wojciechowska Dorota Myszkowska Krystyna Piotrowska-Weryszko Elżbieta Weryszko-Chmielewska Małgorzata Puc Piotr Rapiejko Tomasz Stosik 《Aerobiologia》2018,34(3):301-313
Prediction of allergic pollen concentration is one of the most important goals of aerobiology. Past studies have used a broad range of modeling techniques; however, the results cannot be directly compared owing to the use of different datasets, validation methods, and evaluation metrics. The main aim of this study was to compare nine statistical modeling techniques using the same dataset. An additional goal was to assess the importance of predictors for the best model. Aerobiological data for Corylus, Alnus, and Betula pollen counts were obtained from nine cities in Poland and covered between five and 16 years of measurements. Meteorological data from the AGRI4CAST project were used as a predictor variables. The results of 243 final models (3 taxa \(\times\) 9 cities \(\times\) 9 techniques) were validated using a repeated k-fold cross-validation and compared using relative and absolute performance statistics. Afterward, the variable importance of predictors in the best models was calculated and compared. Simple models performed poorly. On the other hand, regression trees and rule-based models proved to be the most accurate for all of the taxa. Cumulative growing degree days proved to be the single most important predictor variable in the random forest models of Corylus, Alnus, and Betula. Finally, the study suggested potential improvements in aerobiological modeling, such as the application of robust cross-validation techniques and the use of gridded variables. 相似文献
1000.
Interplay of cell–cell contacts and RhoA/MRTF‐A signaling regulates cardiomyocyte identity 下载免费PDF全文
Dorota Zawada Harold Ayetey Gianluca Santamaria Laura Iop Elisa Mastantuono Daniel Sinnecker Alexander Goedel Ralf J Dirschinger Ilaria My Svenja Laue Tarik Bozoglu Christian Baarlink Tilman Ziegler Elisabeth Graf Rabea Hinkel Giovanni Cuda Stefan Kääb Andrew A Grace Robert Grosse Christian Kupatt Thomas Meitinger Austin G Smith Karl‐Ludwig Laugwitz Alessandra Moretti 《The EMBO journal》2018,37(12)