全文获取类型
收费全文 | 12149篇 |
免费 | 303篇 |
国内免费 | 1篇 |
出版年
2021年 | 110篇 |
2020年 | 90篇 |
2019年 | 106篇 |
2018年 | 252篇 |
2017年 | 216篇 |
2016年 | 300篇 |
2015年 | 299篇 |
2014年 | 381篇 |
2013年 | 512篇 |
2012年 | 474篇 |
2011年 | 516篇 |
2010年 | 318篇 |
2009年 | 243篇 |
2008年 | 334篇 |
2007年 | 366篇 |
2006年 | 361篇 |
2005年 | 319篇 |
2004年 | 276篇 |
2003年 | 334篇 |
2002年 | 287篇 |
2001年 | 287篇 |
2000年 | 224篇 |
1999年 | 176篇 |
1998年 | 86篇 |
1992年 | 231篇 |
1991年 | 221篇 |
1990年 | 177篇 |
1989年 | 211篇 |
1988年 | 231篇 |
1987年 | 235篇 |
1986年 | 213篇 |
1985年 | 238篇 |
1984年 | 193篇 |
1983年 | 160篇 |
1980年 | 81篇 |
1979年 | 228篇 |
1978年 | 154篇 |
1977年 | 133篇 |
1976年 | 134篇 |
1975年 | 165篇 |
1974年 | 261篇 |
1973年 | 258篇 |
1972年 | 249篇 |
1971年 | 241篇 |
1970年 | 255篇 |
1969年 | 245篇 |
1968年 | 184篇 |
1967年 | 185篇 |
1966年 | 166篇 |
1965年 | 123篇 |
排序方式: 共有10000条查询结果,搜索用时 578 毫秒
41.
I Urasiński G Uhlenbruck C Dienst 《Folia haematologica (Leipzig, Germany : 1928)》1985,112(3):403-408
The capacity to phagocytosis in monocytic macrophages was investigated in 24 patients with plasmocytoma. The investigation of this capacity was made by using short-term cultures with sheep erythrocytes loaded with antibodies and complement. In comparison to the control group the percentage of phagocyting macrophages was lowered in 16 from 24 patients. This impairment of the function of monocytic macrophages seems to be bound neither to the type of plasmocytoma nor to the stage of disease. Among other things, the nature of cell defect is discussed. The necessity of further investigations is emphasized. 相似文献
42.
M. Cycoń Z. Piotrowska-Seget J. Kozdrój 《World journal of microbiology & biotechnology》2010,26(3):409-418
Changes in microbiological characteristics in response to diazinon, applied at three different dosages of 7, 35 and 700 mg kg?1 soil, were studied in pots filled with sandy soils of different texture. The insecticide dosages corresponded to the maximum predicted environmental concentration (PEC) in field conditions and five or hundred times this rate, respectively. To ascertain these changes, activities of selected soil enzymes, numbers of some microbial populations and bacterial community structure (r/K-strategists) were determined. Acid phosphatase activity and concentrations of ammonium ions either increased or were unaffected by the diazinon dosages. In contrast, nitrate ions, alkaline phosphatase, urease and especially dehydrogenase activities decreased in both soils treated with the higher dosages of the insecticide. The diazinon treatments increased the numbers of culturable bacteria and fungi; however, N-fixing bacteria and nitrifiers (but not denitrifiers) were decreased in both soils. When the high concentration of diazinon (100-times PEC) was added to the loamy sand soil (LS), simulating an undiluted chemical spill, the bacterial community structure shifted towards domination of K-strategists over time. However, r-strategists dominated in the community exposed to the same dosage of the insecticide in the sandy loam soil (SL). Generally, SL soil characteristics were more suitable for microbial activity and growth than those of LS soil were. The results indicate that except a situation of undiluted spill diazinon seems to be a non-hazardous chemical for the culturable soil microorganisms when applied at recommended concentrations. 相似文献
43.
44.
45.
46.
The effect of pteridine derivatives and analogues on the cell outgrowth from the ovarian explants of the waxmoth, Galleria mellonella, was examined in hanging-drop cultures and cytochemical tests were made for succinate and glucose-6-phosphate dehydrogenases. Most of the derivatives and analogues of 2-amino-4-hydroxypteridine injured to a lesser or greater extent insect ovarian tissues in vitro, depending on the drug structure and the concentration applied. Of all the pteridine derivatives and analogues tested only 2-amino-4-mercaptopteridine and its C-6,7-dimethyl derivative (10 μM) promoted cell outgrowth from the explants as did folate. 相似文献
47.
48.
49.
50.
Stanislaw Kowalczyk Anna Jakubowska Elbieta Zieliska Robert S. Bandurski 《Physiologia plantarum》2003,119(2):165-174
1- O -(indole-3-acetyl)- β - d -glucose: myo -inositol indoleacetyl transferase (IA- myo -inositol synthase) is an important enzyme in IAA metabolism. This enzyme catalyses the transfer of the indole acetyl (IA) moiety from 1- O -(indole-3-acetyl)- β - d -glucose to myo -inositol to form IA- myo- inositol and glucose. IA- myo -inositol synthase was purified to an electrophoretically homogenous state from maize liquid endosperm by fractionation with ammonium sulphate, anion-exchange, adsorption on hydroxylapatite, affinity chromatography on ConA-Sepharose, preparative PAGE and isoelectric focusing. We thus obtained two enzyme preparations which differ in their R f on 8% polyacrylamide gel. The preparation of R f 0.36 contained a single 56.4 kDa polypeptide, whereas the preparation of R f 0.39 consisted of two polypeptides of 56.4 and 53.5 kDa. Both purified preparations of IAInos synthase also exhibited the activity of an IAInos hydrolase, showing that the dual activity was associated with a single protein. Results of gel filtration and analytical SDS-PAGE suggest that the native enzyme exists as both a monomeric (65 kDa) and homo- or heterodimeric form (110–130 kDa). Analysis of peptide maps and amino acid sequences of two 21 amino-acid peptides showed that polypeptides of 56.4 and 53.5 kDa have the same primary structure and that the 3 kDa difference in molecular mass is probably caused by different glycosylation levels. Comparison of this partial and internal amino acid sequence with sequences of other plant acyltransferases indicated similarity to several proteins which belonged to the serine carboxypeptidase-like (SCPL) acyltransferase family. 相似文献