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71.
van Dooren TJ 《Proceedings. Biological sciences / The Royal Society》2001,268(1464):279-287
Two versions of a model for the evolution of seasonal polyphenism investigate the evolution of reaction norm bifurcation and branching. The first version is without a specific submodel for morphological development and the second has an explicit developmental map. Version 1 is evolutionarily relatively unconstrained: (i) reaction norms are specified by matrices containing the probabilities of occurrence of environment-phenotype combinations, (ii) all conceivable reaction norm matrices are reachable through a sequence of mutations, and (iii) small as well as large mutational effects occur. This version is used to find the evolutionarily stable strategy favoured by the population ecology that is characterized by stabilizing viability selection with a cyclically fluctuating selection optimum. When the strength of selection is large and when the lag between initiation of development and selection on mature phenotype is not a multiple of half the period of the environmental cycle, a branching reaction norm evolves. In the second model version, branching reaction norms occur for certain parameter combinations of the developmental submodel, but the evolution of this pattern is often constrained. The evolutionary trajectory becomes trapped in a local selective optimum for the parameters of the developmental system. Substantial developmental noise evolves, but mutations that produce a selectively advantageous branching pattern do not occur from there. 相似文献
72.
Sally D. Warring Zhicheng Dou Vern B. Carruthers Geoffrey I. McFadden Giel G. van Dooren 《Eukaryotic cell》2014,13(11):1360-1370
Mutations in the Plasmodium falciparum chloroquine resistance transporter (PfCRT) protein confer resistance to the antimalarial drug chloroquine. PfCRT localizes to the parasite digestive vacuole, the site of chloroquine action, where it mediates resistance by transporting chloroquine out of the digestive vacuole. PfCRT belongs to a family of transporter proteins called the chloroquine resistance transporter family. CRT family proteins are found throughout the Apicomplexa, in some protists, and in plants. Despite the importance of PfCRT in drug resistance, little is known about the evolution or native function of CRT proteins. The apicomplexan parasite Toxoplasma gondii contains one CRT family protein. We demonstrate that T. gondii CRT (TgCRT) colocalizes with markers for the vacuolar (VAC) compartment in these parasites. The TgCRT-containing VAC is a highly dynamic organelle, changing its morphology and protein composition between intracellular and extracellular forms of the parasite. Regulated knockdown of TgCRT expression resulted in modest reduction in parasite fitness and swelling of the VAC, indicating that TgCRT contributes to parasite growth and VAC physiology. Together, our findings provide new information on the role of CRT family proteins in apicomplexan parasites. 相似文献
73.
Tom J. M. Van Dooren 《Ecology and evolution》2019,9(23):13056-13068
Estimating and predicting temporal trends in species richness is of general importance, but notably difficult because detection probabilities of species are imperfect and many datasets were collected in an opportunistic manner. We need to improve our capabilities to assess richness trends using datasets collected in unstandardized procedures with potential collection bias. Two methods are proposed and applied to estimate richness change, which both incorporate models for sampling effects and detection probability: (a) nonlinear species accumulation curves with an error variance model and (b) Pradel capture–recapture models. The methods are used to assess nationwide temporal trends (1945–2018) in the species richness of wild bees in the Netherlands. Previously, a decelerating decline in wild bee species richness was inferred for part of this dataset. Among the species accumulation curves, those with nonconstant changes in species richness are preferred. However, when analyzing data subsets, constant changes became selected for non‐Bombus bees (for samples in collections) and bumblebees (for spatial grid cells sampled in three periods). Smaller richness declines are predicted for non‐Bombus bees than bumblebees. However, when relative losses are calculated from confidence intervals limits, they overlap and touch zero loss. Capture–recapture analysis applied to species encounter histories infers a constant colonization rate per year and constant local species survival for bumblebees and other bees. This approach predicts a 6% reduction in non‐Bombus species richness from 1945 to 2018 and a significant 19% reduction for bumblebees. Statistical modeling to detect species richness time trends should be systematically complemented with model checking and simulations to interpret the results. Data inspection, assessing model selection bias, and comparisons of trends in data subsets were essential model checking strategies in this analysis. Opportunistic data will not satisfy the assumptions of most models and this should be kept in mind throughout. 相似文献
74.
Majeed Askari Seyahooei Femmie J. L. Kraaijeveld‐Smit Ken Kraaijeveld Jessica B. M. Crooijmans Tom J. M. Van Dooren Jacques J. M. Van Alphen 《Oikos》2009,118(8):1148-1157
Few examples exist where parasites manipulate host behaviour not to increase their transmission rate, but their own survival. Here we investigate fitness effects of parasitism by Asobara species in relation to the pupation behaviour of the host, Drosophila melanogaster . We found that Asobara citri parasitized larvae pupate higher in rearing jars compared to unparasitized controls, while A. tabida pupated on or near the medium. No change in pupation site was found for three other species. A follow-up experiment showed a non-random distribution of parasitized and unparasitized pupae over the different jar parts. To test the adaptiveness of these findings, we performed pupal transfer experiments. Optimum pupation sites were found to be different between host individuals; wall individuals survived better than bottom individuals, but bottom individuals did worse at the wall. Two parasitoid species that alter pupation site significantly showed high rates of diapause at their 'preferred' pupation site. For one of them, A. citri , pupation occurred at the optimal site for highest survival (emergence plus diapause). From literature we know that pupation height and foraging activity are genetically positively linked. Therefore, we implement a short assay for rover/sitter behavioural expression by measuring distance travelled during foraging after parasitism. For one out of three species, foraging activity was reduced, suggesting that this species suppresses gene expression in the for pathway and thereby reduces pupation height. The parasitoid species used here, naturally inhabit widely different environments and our results are partly consistent with a role for ecology in shaping the direction of parasite-induced changes to host pupation behaviour. More parasitoids are found on the wall of the rearing jar when they originate from dry climates, while parasitoids from wet climates pupate on the humid bottom. 相似文献
75.
Swati Agrawal Giel G. van Dooren Wandy L. Beatty Boris Striepen 《The Journal of biological chemistry》2009,284(48):33683-33691
Most apicomplexan parasites harbor a relict chloroplast, the apicoplast, that is critical for their survival. Whereas the apicoplast maintains a small genome, the bulk of its proteins are nuclear encoded and imported into the organelle. Several models have been proposed to explain how proteins might cross the four membranes that surround the apicoplast; however, experimental data discriminating these models are largely missing. Here we present genetic evidence that apicoplast protein import depends on elements derived from the ER-associated protein degradation (ERAD) system of the endosymbiont. We identified two sets of ERAD components in Toxoplasma gondii, one associated with the ER and cytoplasm and one localized to the membranes of the apicoplast. We engineered a conditional null mutant in apicoplast Der1, the putative pore of the apicoplast ERAD complex, and found that loss of Der1Ap results in loss of apicoplast protein import and subsequent death of the parasite. 相似文献
76.
Differential Membrane Association Properties and Regulation of Class I and Class II Arfs 总被引:1,自引:0,他引:1
Daniël Duijsings Kjerstin H. W. Lanke Sander H. J. van Dooren Michiel M. T. van Dommelen Roy Wetzels Fabrizio de Mattia Els Wessels Frank J. M. van Kuppeveld 《Traffic (Copenhagen, Denmark)》2009,10(3):316-323
ADP-ribosylation factor (Arf) proteins are small guanosine triphosphatases (GTPases) that act as major regulators of intracellular vesicular trafficking and secretory organelle pathway integrity. Like all small monomeric GTPases, Arf proteins cycle between a GDP-bound and a GTP-bound state, and this cycling is catalysed by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins. While the class I Arfs, especially Arf1, have been studied extensively, little is known as yet about the function and regulation of class II Arfs, Arf4 and Arf5. In this study, we show that Arf proteins show class-specific dynamic behaviour. Moreover, unlike class I Arfs, membrane association of class II Arfs is resistant to inhibition of large Arf GEFs by Brefeldin A. Through the construction of Arf chimeric proteins, evidence is provided that the N-terminal amphipathic helix and a class-specific residue in the conserved interswitch domain determine the membrane-binding properties of class I and class II Arf proteins. Our results show that fundamental differences exist in behaviour and regulation of these small GTPases. 相似文献
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L. E. den Dooren de Jong 《Archives of microbiology》1938,9(1-5):223-252
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