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111.
Background
Microsporidia are intracellular parasites that are highly-derived relatives of fungi. They have compacted genomes and, despite a high rate of sequence evolution, distantly related species can share high levels of gene order conservation. To date, only two species have been analysed in detail, and data from one of these largely consists of short genomic fragments. It is therefore difficult to determine how conservation has been maintained through microsporidian evolution, and impossible to identify whether certain regions are more prone to genomic stasis.Principal Findings
Here, we analyse three large fragments of the Enterocytozoon bieneusi genome (in total 429 kbp), a species of medical significance. A total of 296 ORFs were identified, annotated and their context compared with Encephalitozoon cuniculi and Antonospora locustae. Overall, a high degree of conservation was found between all three species, and interestingly the level of conservation was similar in all three pairwise comparisons, despite the fact that A. locustae is more distantly related to E. cuniculi and E. bieneusi than either are to each other.Conclusions/Significance
Any two genes that are found together in any pair of genomes are more likely to be conserved in the third genome as well, suggesting that a core of genes tends to be conserved across the entire group. The mechanisms of rearrangments identified among microsporidian genomes were consistent with a very slow evolution of their architecture, as opposed to the very rapid sequence evolution reported for these parasites. 相似文献112.
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Transcriptional control of brown fat determination by PRDM16 总被引:1,自引:0,他引:1
Seale P Kajimura S Yang W Chin S Rohas LM Uldry M Tavernier G Langin D Spiegelman BM 《Cell metabolism》2007,6(1):38-54
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Bradley D. Tait John Domagala Edmund L. Ellsworth Donna Ferguson Christopher Gajda Donald Hupe Elizabeth A. Lunney Peter J. Tummino 《Journal of molecular recognition : JMR》1996,9(2):139-142
New templates were designed and prepared which straddle the active site of HIV-1 protease. These templates were designed to be ‘flexible scaffolds’ upon which substituents could be appended to fill the pockets of HIV protease. The new templates prepared and analysed were 4-hydroxy-5H-furan-2-ones, 4-hydroxy-5,6-dihydropyrones, 3-hydroxy-cyclohex-2-enones, and 4-hydroxy-2(1H)-pyridinones, of which the 4-hydroxy- 5,6-dihydropyrones were found to be the most potent inhibitors of HIV-1 protease. 相似文献
118.
The amino acid sequence of a Bacillus subtilis phosphoprotein that matches an orfY-tsr coding sequence 总被引:1,自引:0,他引:1
Clayton Mitchell Paul W. Morris Leanna Lum George Spiegelman James C. Vary 《Molecular microbiology》1992,6(10):1345-1349
Bacillus subtilis contains a 30 kDa protein which was phosphorylated during late vegetative growth and sporulation. The sequence for the N-terminal 16 amino acids was found to be identical to the predicted sequence for the N-terminus of a small open reading frame, orfY, but diverged from the predicted sequence thereafter. The orfY region was resequenced and contained one less adenine residue than previously reported, resulting in an open reading frame from within orfY through the entire coding region for tsr which follows orfY. The predicted orfY-tsr amino acid sequence showed 24% identity to Escherichia coli fructose-1,6-bisphosphate aldolase. Two mutants in the tsr region had 2-5% of wild-type aldolase and the nucleotide sequences showed missense mutations. These results indicate that orfY-tsr encodes aldolase and should be renamed fba1. 相似文献
119.
Anubama Rajan Felipe-Andrs Piedra Letisha Aideyan Trevor McBride Matthew Robertson Hannah L. Johnson Gina Marie Aloisio David Henke Cristian Coarfa Fabio Stossi Vipin Kumar Menon Harshavardhan Doddapaneni Donna Marie Muzny Sara Joan Javornik Cregeen Kristi Louise Hoffman Joseph Petrosino Richard A. Gibbs Vasanthi Avadhanula Pedro A. Piedra 《Journal of virology》2022,96(7)
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