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81.
Triiodothyronine (T3) is found to stimulate cytochalasin B-inhibitable glucose transport in Clone 9 cells, a 'non-transformed' rat liver cell line. After an initial lag period of more than 3 h, glucose transport rate is significantly increased at 6 h and reaches more than 3-times the control rate at 24 h. The enhancement of glucose transport by T3 is due to an increase in transport Vmax and occurs in the absence of a change in either the Km for glucose transport (approximately 3 mM) or the Ki for inhibition of transport by cytochalasin B ((1-2).10(-7) M). Consistent with the observed Ki for cytochalasin B, Northern blot analysis of RNA from control and T3-treated cells employing cDNA probes encoding GTs of the human erythrocyte/rat brain/HepG2 cell transporter (GLUT-1), rat muscle/fat cell transporter (GLUT-4), and rat liver transporter (GLUT-2) types indicates expression of only the GLUT-1 mRNA isoform in these cells. The abundance of GLUT-1 mRNA increases approx. 1.9-fold after 24 h of T3 treatment and is accompanied by an approx. 1.3-fold increase in the abundance of GLUT-1 in whole-cell extracts as demonstrated by Western blot analysis employing a polyclonal antibody directed against the 13 amino acid C-terminal peptide of GLUT-1. The more than 3-fold stimulation of glucose transport at 24 h substantially exceeds the fractional increment in transporter abundance suggesting that, in addition to increasing total GLUT-1 abundance, exposure to T3 may result in a translocation of transporters to the plasma membrane or an activation of pre-existing membrane transporter sites. 相似文献
82.
S T Butera V L Perez N J Besansky W C Chan B Y Wu G J Nabel T M Folks 《Journal of cellular biochemistry》1991,45(4):366-373
In this report, we describe a human immunodeficiency virus type-1 (HIV-1)-infected promyelocytic cell line, OM, derived from HL-60 cells. Although the OM cell line was biologically cloned twice, the pattern of HIV-1 expression during culture appeared analogous to a classical acute spreading infection and was inhibited by both azidothymidine and recombinant soluble CD4 treatment. The number of OM cells actually expressing HIV-1 at the beginning of culture was 0%, reached a peak of nearly 100% at 6 weeks, and then fell to less than 10% HIV-1+ cells by 10 weeks. Clonal analysis of the surviving cells verified that stable HIV-1+ OM cells resulted from the spreading infection. Southern analysis confirmed the transmission of HIV-1 through these OM cultures and the occurrence of stable clones which resulted. The initial percentage of OM cells actually harboring the HIV-1 genome was less than 0.1%, indicating nonfaithful transmission of an unintegrated HIV-1 genome during clonal expansion. These results demonstrate that extrachromosomal HIV-1 DNA can contribute to the spread of HIV-1 infection and give rise to cells which have stably integrated HIV-1 provirus. 相似文献
83.
The uptake of morphine was significantly reduced in most regions of the brains of conscious, unrestrained rats within 10 minutes after treatment with an analog of ACTH/MSH (4–9), ORG-2766. The effect was most obvious in regions with significant densities of enkephalin receptors, namely basal ganglia, hippocampus and cortex. The results explain, in part, how some fragments and analogs of ACTH/MSH may antagonize behavioral actions of morphine, even though some of these peptides lack significant opiate receptor binding properties. We believe that this effect of ORG-2766 is related to an action on the permeability characteristics of the brain microvasculature. The underlying mechanism is unknown. 相似文献
84.
Restrictive control (“repression”) of 3-O-methylglucose transport (or of galactose uptake) in confluent NIL hamster fibroblast cultures was found to be highly pronounced after preconditioning the cultures in medium containing d-glucosamine. The “repression” exerted by glucosamine developed slowly over several hours. The transport “repression” was counteracted by anaerobiosis, by 2,4-dinitrophenol (H. M. Kalckar, C. W. Christopher, and D. Ullrey, 1979, Proc. Nat. Acad. Sci. USA76, 6453–6455), and by fluoride as well as by malonate. In “de-repressed” cultures, i.e., in the absence of glucosamine in the medium or by using fructose during preconditioning, malonate did not affect regulation of the hexose transport system. In culture medium deprived of l-glutamine and serum, repressive control of the transport system by glucose as well as by glucosamine was greatly aggravated. However, the simultaneous addition of malonate abolished the severe “repression” by either of the hexoses. In all cases, preconditioning with fructose permitted high (“de-repressed”) transport activity. Unlike glucose, galactose, or glucosamine, fructose was not found to compete in the transport assay. The metabolic inhibitors which prevent the aerobic curtailment of the hexose transport system are all more or less directly interfering with the flow of metabolites through the tricarboxylate cycle, which may therefore play an important role in the “repressive” control of transport. 相似文献
85.
Francine C. Eden Anna Maria Musti Donna A. Sobieski 《Journal of molecular biology》1981,148(2):129-151
In the chicken genome there are middle repetitive DNA sequences with a clustered organization. Each cluster is composed of members of different families of repeated DNA sequences and usually contains only one member of each family. Many clusters have the same assortment of repeated sequences but they are in scrambled order from cluster to cluster. These clusters usually exceed 20 × 103 bases in length and comprise at least 10% of the repeated DNA of the chicken. The repeated sequences that are cluster components are extensively methylated. Methylation was detected by comparing HpaII and MspI digests of total DNA, where the occurrence of the sequence C-m5C-G-G is indicated when HpaII (cleaves C-C-G-G) fragments are larger than those generated by MspI (cleaves C-m5C-G-G or C-C-G-G). In hybridization experiments with Southern (1975) blots of total DNA digested with either HpaII or MspI, the cloned probes representing clustered repeated sequences showed a dramatic difference in the lengths of restriction fragments detected in the two digests. Many of the sequences that comprise these clusters are methylated in most of their genomic occurrences. There are patterns of methylation that are reproduced faithfully from copy to copy. The overall distribution of methylation within clusters seems to be regional, with long methylated DNA segments interrupted by specific undermethylated regions. 相似文献
86.
De La Calzada CS Verdugo AL Perez JT Alcaine CC Fernandez AS 《Cardiovascular diseases》1981,8(1):73-83
A 50-year-old man presented with hypertrophic obstructive cardiomyopathy (HOC) associated with a left ventricular aneurysm and normal coronary arteries. His history revealed no evidence of myocardial infarction or atypical angina. Physical examination disclosed HOC but did not suggest the presence of an aneurysm. Although the patient was treated medically, heart failure ensued, and he died suddenly while working his farm. Subsequent investigation of the patient's family revealed that three of his five children were also affected by cardiomyopathy, which was especially pronounced in the eldest, a 22-year-old man. The possible hemodynamic relationship between HOC and left ventricular aneurysm is discussed, along with probable indications. The role of left ventricular aneurysm is also presented in relation to the natural history of the disease. 相似文献
87.
Population Ecology - Female deermice housed from weaning with groups of five females, five males or five males plus five females had significantly smaller uteri at 35–38 days of age compared... 相似文献
88.
Surface staining of the intact vascular endothelial cell layer lining the lumen of guinea pig thoracic aorta with antibodies to vimentin revealed that at least 70% of the cells contained intact perinuclear rings of 10-nm filaments. This correlated with the observations made on these cells in culture: 60–80% of the endothelial cells at confluence have complete perinuclear rings. By one- and two-dimensional gel electrophoresis and immunoprecipitation we confirmed that vimentin [17, 18] is the major constituent polypeptide of the 10-nm filaments in guinea pig endothelial cells. These results indicate that the vimentin [17] 10-nm filament cytoskeleton found in guinea pig endothelial cells in vitro is similar to the cytoskeleton found in situ. 相似文献
89.
In a mixotrophic environment, Thiobacillus novellus concurrently utilized glucose and thiosulfate but showed no stimulation of growth rate or yield. In most mixotrophic environments examined, the growth rate was lower than the heterotrophic growth rate, the extent of the decrease depending on the concentration and relative proportion of thiosulfate and glucose in the medium. Both thiosulfate and glucose were degraded to their most oxidized products in mixotrophic medium, yet the biomass production in this medium was comparable to that found in heterotrophic medium containing glucose alone at the corresponding concentration. It was postulated that in mixotrophic medium the oxidation of thiosulfate, glucose, or partially that of both was uncoupled from energy generation. These results differ in many respects from those reported earlier by LeJohn et al. (J. Bacteriol. 94: 1484--1491, 1967); experiments designed to exactly duplicate some of the growth conditions employed by these workers did not resolve the discrepancy. 相似文献
90.
Lytic enzymes in the autolysis of filamentous fungi 总被引:4,自引:0,他引:4
The degrees of autolysis attained by five different genera of filamentous fungi during an incubation period of 60 days, under the same culture conditions were: 87.3% for Penicillium oxalicum; 65.9% for Neurospora crassa; 62.7% for Polystictus versicolor; 51.7% for Aspergillus niger and 23.5% for Nectria galligena. N. crassa, A. niger and P. versicolor reached the end of the autolysis during this incubation period (60 days), whereas P. oxalicum and N. galligena did not.The excretion of the lytic enzymes -N-acetyl-glucosaminidase, -1–3 glucanase, chitinase, invertase and acid phosphatase into the culture medium during growth and autolysis was investigated. The excretion of these enzymes was consistent with the degree of autolysis reached, the maximum excretion belonging to P. oxalicum and the minimum to N. galligena. The N. crassa invertase was excreted into the culture liquid at levels very much higher than the other enzymes studied, and at levels very much higher than the invertases excreted by the other fungi. 相似文献