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991.
Bruce CR Smith DA Rodgers D da Silva Dantas A MacCallum DM Morgan BA Quinn J 《PloS one》2011,6(12):e27979
Candida albicans colonises numerous niches within humans and thus its success as a pathogen is dependent on its ability to adapt to diverse growth environments within the host. Two component signal transduction is a common mechanism by which bacteria respond to environmental stimuli and, although less common, two component-related pathways have also been characterised in fungi. Here we report the identification and characterisation of a novel two component response regulator protein in C. albicans which we have named CRR1 (Candida Response Regulator 1). Crr1 contains a receiver domain characteristic of response regulator proteins, including the conserved aspartate that receives phosphate from an upstream histidine kinase. Significantly, orthologues of CRR1 are present only in fungi belonging to the Candida CTG clade. Deletion of the C. albicans CRR1 gene, or mutation of the predicted phospho-aspartate, causes increased sensitivity of cells to the oxidising agent hydrogen peroxide. Crr1 is present in both the cytoplasm and nucleus, and this localisation is unaffected by oxidative stress or mutation of the predicted phospho-aspartate. Furthermore, unlike the Ssk1 response regulator, Crr1 is not required for the hydrogen peroxide-induced activation of the Hog1 stress-activated protein kinase pathway, or for the virulence of C. albicans in a mouse model of systemic disease. Taken together, our data suggest that Crr1, a novel response regulator restricted to the Candida CTG clade, regulates the response of C. albicans cells to hydrogen peroxide in a Hog1-independent manner that requires the function of the conserved phospho-aspartate. 相似文献
992.
993.
Red-backed salamanders, Plethodon cinereus, were paired in laboratory chambers where they defended areas against each other by threat display and biting. Difference in sizes of paired individuals was not correlated with which one would enter the other's area or be more aggressive after an intrusion. We also found no sexual differences in agonistic behaviour. Both defenders and intruders assumed a threat posture for about 50% of the invasion periods, but defenders exhibited a higher rate of biting. Defenders expelled intruders in 74% of all encounters, while intruders expelled defenders in 18%, with 8% of the encounters a draw. Intruders frequently left the contested area even in the absence of biting or threat display by the defender, apparently in response to the defender's pheromones or to the high potential cost of an escalated aggressive contest (damage to the chemosensory structures). These data provide behavioural evidence that a salamander can evict intruders from a defended area and thus support the hypothesis of territoriality among terrestrial salamanders. 相似文献
994.
995.
The effects of the naturally occurring polyamines, spermine, putrescine, and spermidine were explored on mitochondrial state 3. state 4, and uncoupled respiration activities, ADP/O ratio, respiratory control ratio of pepper ( Capsicum annuum L. cv. Early Cal Wonder) and avocado ( Persea americana Mill. cv. Booth-8 or Simmonds) mitochondria oxidizing either succinate, external NADH, malate, α-ketoglutarate or tetramethyl- p -phenylenediamine. Abnormally high concentrations of spermine and spermidine such as might occur during chilling stress of these chilling-sensitive fruits were detrimental to several oxidase activities, especially to external NADH oxidase. State 3 respiration for NADH oxidase was inhibited more than 70% by 10 m M spermine. The spermine inhibition of uncoupled NADH oxidase was not reversed by the presence of divalent cations including Ca2+ , Mg2+ , Mn2+ , and Sr2+ at concentrations up to 10 m M or by 100 m M KCl. The inhibition primarily affected the Vmax . Other possible sites of polyamine interactions are discussed. 相似文献
996.
997.
Ildiko Lingvay Kirstine Brown-Frandsen Helen M. Colhoun John Deanfield Scott S. Emerson Sille Esbjerg Søren Hardt-Lindberg G. Kees Hovingh Steven E. Kahn Robert F. Kushner A. Michael Lincoff Steven P. Marso Tea Monk Fries Jorge Plutzky Donna H. Ryan the SELECT Study Group 《Obesity (Silver Spring, Md.)》2023,31(1):111-122
998.
Poor developmental spike synchrony in wheat (Triticum aestivum L.) can reduce the effectiveness of chemical treatments keyed on reproductive events. The broadleaf herbicide (2-methyl-4-chlorophenoxy) acetic acid (MCPA) can be used to retard the development of wheat tillers if applied to winter wheat in the fall prior to the initiation of tiller primordia. Fall applications of 0.5 kg ha–1 MCPA were sufficient to reduce the tillering rate by 20–30% while providing a slight, but statistically non-significant, increase in yield. Significant increases in kernels spike–1 were observed in the MCPA treatments. The effect of MCPA on kernels spike –1 could be modulated by nitrogen fertility and planting density. A linear relationship between spike number m–2 and planting density could be observed with MCPA treatments. Reductions in total number of spikes m–2, but an increase in kernels spike–1, resulted in significant improvements in tiller synchrony. Improved tiller synchrony is important in optimizing chemical treatments where applications are based on the developmental stages of the spike. Significant reductions in plant height and subsequent reductions in lodging under high nitrogen fertility and high plant populations were observed with MCPA treatments. 相似文献
999.
R De Luca A Renzulli M R Cafaro A Officioso R D'Alessandro C Sellitto V Di Donna 《Bollettino della Società italiana di biologia sperimentale》1984,60(4):745-748
In this research we have determined the behaviour of hypophisary hormones determined by radioimmunoassay. We have noted a constant increase of GH and HPrL. In one case we have seen also the decrease of LH and FSH. This changes are determined by the large dose of heparin necessary during C.E.C. 相似文献
1000.
Caroline C. Whitacre David H. Mattson Philip Y. Paterson Raymond P. Roos Donna J. Peterson Barry G. W. Arnason 《Neurochemical research》1981,6(1):87-96
Rabbits sensitized with whole nervous tissue or myelin basic protein (MBP) plus adjuvant and developing experimental allergic encephalomyelitis (EAE) were studied for the presence of oligoclonal immunoglobulin (Ig) bands in spinal fluid and serum. Samples obtained prior to sensitization and at the time of sacrifice were concentrated and subjected to agar gel electrophoresis. Of 11 rabbits receiving whole nervous tissue and developing severe clinical signs of EAE, 7 showed new oligoclonal Ig bands in spinal fluid and in serum obtained 19 days or more after sensitization. With MBP sensitization, 2 of 6 rabbits exhibited new spinal fluid bands, while all 6 rabbits studied demonstrated serum banding. The bands were identified as IgG by immunochemical studies using peroxidase-labeled antisera and byStaph. protein A absorption. The majority of animals showed no banding in presensitization samples. The finding of oligoclonal IgG in EAE reveals yet another immunologic correlation between EAE and the human demyelinating disease, multiple sclerosis. 相似文献