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71.
72.
Zhang Nan Gao Xiaochen Zhang Weijia Higazy Doaa Wang Ke Fu Zhenfang Cui Min 《Molecular biology reports》2020,47(2):1393-1398
Molecular Biology Reports - Peroxynitrite is an ion acting as a powerful oxidant and nucleophile, which plays a key role in the inflammation and aging process by nitrating tyrosine or tryptophan... 相似文献
73.
Chen Jiajia Yang Saishuai Wu Chunshuai Cui Zhiming Wan Yangyang Xu Guanhua Bao Guofeng Zhang Jinlong Chen Chu Song Dianwen 《Neurochemical research》2020,45(10):2302-2311
Neurochemical Research - Spinal cord injury (SCI) is one of the diseases with high probability of causing disability in human beings, and there is no reliable treatment at present. Neuronal... 相似文献
74.
Qiuxia Cui Jianing Tang Dan Zhang Deguang Kong Xing Liao Jiangbo Ren Yan Gong Conghua Xie Gaosong Wu 《Journal of cellular biochemistry》2020,121(8-9):3923-3934
Breast cancer is a popularly diagnosed malignant tumor. Genomic profiling studies suggest that breast cancer is a disease with heterogeneity. Chemotherapy is one of the chief means to treat breast cancer, while its responses and clinical outcomes vary largely due to the conventional clinicopathological factors and inherent chemosensitivity of breast cancer. Using the least absolute shrinkage and selection operator (LASSO) Cox regression model, our study established a multi-mRNA-based signature model and constructed a relative nomogram in predicting distant-recurrence-free survival for patients receiving surgery and following chemotherapy. We constructed a signature of eight mRNAs (IPCEF1, SYNDIG1, TIGIT, SPESP1, C2CD4A, CLCA2, RLN2, and CCL19) with the LASSO model, which was employed to separate subjects into groups with high- and low-risk scores. Obvious differences of distant-recurrence-free survival were found between these two groups. This eight-mRNA-based signature was independently associated with the prognosis and had better prognostic value than classical clinicopathologic factors according to multivariate Cox regression results. Receiver operating characteristic results demonstrated excellent performance in diagnosing 3-year distant-recurrence by the eight-mRNA signature. A nomogram that combined both the eight-mRNA-based signature and clinicopathological risk factors was constructed. Comparing with an ideal model, the nomograms worked well both in the training and validation sets. Through the results that the eight-mRNA signature effectively classified patients into low- and high-risk of distant recurrence, we concluded that this eight-mRNA-based signature played a promising predictive role in prognosis and could be clinically applied in breast cancer patients receiving adjuvant chemotherapy. 相似文献
75.
Zheng Wang Zhongmin Wang Lu Gao Lili Xiao Rui Yao Binbin Du Yapeng Li Leiming Wu Cui Liang Zhen Huang Pengcheng Li Yanzhou Zhang 《Journal of cellular physiology》2020,235(3):2149-2160
miR-222 participates in many cardiovascular diseases, but its effect on cardiac remodeling induced by diabetes is unclear. This study evaluated the functional role of miR-222 in cardiac fibrosis in diabetic mice. Streptozotocin (STZ) was used to establish a type 1 diabetic mouse model. After 10 weeks of STZ injection, mice were intravenously injected with Ad-miR-222 to induce the overexpression of miR-222. miR-222 overexpression reduced cardiac fibrosis and improved cardiac function in diabetic mice. Mechanistically, miR-222 inhibited the endothelium to mesenchymal transition (EndMT) in diabetic mouse hearts. Mouse heart fibroblasts and endothelial cells were isolated and cultured with high glucose (HG). An miR-222 mimic did not affect HG-induced fibroblast activation and function but did suppress the HG-induced EndMT process. The antagonism of miR-222 by antagomir inhibited HG-induced EndMT. miR-222 regulated the promoter region of β-catenin, thus negatively regulating the Wnt/β-catenin pathway, which was confirmed by β-catenin siRNA. Taken together, our results indicated that miR-222 inhibited cardiac fibrosis in diabetic mice via negatively regulating Wnt/β-catenin-mediated EndMT. 相似文献
76.
Depending on the microenvironment, macrophages can acquire distinct functional phenotypes, referred to as classically activated M1 and M2. M1 macrophages are considered potent effector cells that kill intracellular pathogens, and M2 macrophages promote the resolution of wound healing. In this study, we are interested to know whether probiotic Bacillus amyloliquefaciens (Ba) can induce macrophages polarization. Real-time fluorescence PCR analysis demonstrated that the expression of IL-1β, iNOS, TNF-α and IL-6 genes for M1 macrophages was significantly increased at 1.5 h after probiotic Ba treatment compared to the probiotic Ba-free treatment (P < 0.01), whereas the expression of M2 macrophage marker genes (Arg1, Fizz1, MR, Ym1) was decreased (P < 0.05). Furthermore, the phagocytic activity was dramatically increased in the Ba-treated BMDMs using a FITC-dextran endocytosis assay. Together, these findings indicated that probiotic Ba facilitated polarization of M1 macrophages and enhanced its phagocytic capacity. The results expanded our knowledge about probiotic function-involved macrophage polarization. 相似文献
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78.
Molecular dynamics simulations have been carried out for simple electrolyte systems to study the electrokinetically driven osmotic flow in parallel-plate channels of widths ~10–120?nm. The results are compared with the classical theory predictions based on the solution to the Poisson–Boltzmann equation. We find that despite some of the limitations in the Poisson–Boltzmann equation, such as assumption of the Boltzmann distribution for the ions, the classical theory captures the general trend of the variations of the osmotic flow with channel width, as characterized by the mobility of the fluid in channels between ~10 and 120?nm at moderate to low ion concentration. At moderate concentration (corresponding to relatively low surface potential), the classical theory is almost quantitative. The theory and simulation show more disagreement at low concentration, primarily caused by the high surface potential where the assumption of Boltzmann distribution becomes inaccurate. We discuss the limitations of the Poisson–Boltzmann equation as applied to the nanoscale channels. 相似文献
79.
Rena Li Ping He Jie Cui Matthias Staufenbiel Nobuhiro Harada Yong Shen 《Molecular neurobiology》2013,47(3):857-867
Estrogens have been found to improve memory and reduce risk of dementia, although conflicting results such as failure of estrogen replacement therapy for treatment of Alzheimer's disease (AD) also has been reported. Only recently, our published human brain studies showed a depletion of brain estrogen in women with AD, while other studies have demonstrated cognitive impairment believed to be caused by inhibition of endogenous estrogen synthesis in females. To investigate whether the shortage of brain estrogen alters the sensitivity of response to estrogen replacement therapy, we have used genetic and surgical animal models to examine the response of estrogen treatment in AD neuropathology. Our studies have shown that early treatment with 17β-estradiol (E2) or genistein could reduce brain amyloid levels by increasing Aβ clearance in both APP23 mice with genetic deficiency of aromatase (APP/Ar+/?), in which the brains contain nondetectable levels of estrogen, and in APP23 mice with an ovariectomy (APP/OVX), in which the brains still contain certain levels of estrogen. However, only APP/Ar+/? mice showed a great reduction in brain amyloid plaque formation after E2 or genistein treatment along with downregulation of β-secretase (BACE1) mRNA and protein expression. Our results suggest that early and long-term usage of E2 and/or genistein may prevent AD pathologies in a dependent manner on endogenous brain estrogen levels in aged females. 相似文献
80.
Chuan-yu Guo Guang-heng Wu Jin Xing Wen-qi Li Ding-zhong Tang Bai-ming Cui 《Plant cell reports》2013,32(5):687-702