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91.
用多孔强碱型三乙醇胺基聚苯乙烯阴离子交换树脂做为载体,用CNBr与载体上的多羟基作用共价偶联了胰酶。红外光谱表明:其共价偶联反应机理与用CNBr活化多糖类载体并接酶的机理相类似。最适偶联条件研究表明:CNBr用量增多,酶蛋白载量增加。但比活下降。偶联pH为10时,固定化酶有适宜的载量和较高的比活。由于胰酶水解蛋白反应释放出H~+质子,这些质子在载体内积累,使微环境内H~+质子浓度增加,进而使得固定化胰酶的pH—活性曲线在pH9~11范围内未出现下降。在变温和60℃恒温下对固定化酶的热稳定性测试表明:固相酶的热稳定性比天然酶的热稳定性有所提高。 相似文献
92.
陕南茶树栽培区域生态与地方品种分布 总被引:1,自引:0,他引:1
陕南秦巴山区是亚热带北缘地区。由于生态环境复杂,地区差异性大,茶树栽培与引种受到一定限制。大致以汉江谷地粮作区为界,南部大巴山北麓低山丘陵区为适栽区;北部秦岭南麓低山丘陵区则为次适栽区。地方茶树群体品种有7个,约由28个地方品种组成,呈斑、块状分布。 相似文献
93.
94.
Acetylcholine in the posterior hypothalamic nucleus is involved in the elevated blood pressure in the spontaneously hypertensive rat 总被引:1,自引:0,他引:1
Intravenous injection of physostigmine, 40 and 80 ug/kg, in unanesthetized normotensive rats increased systolic blood pressure (SBP) by 21 +/- 3 and 42 +/- 7 mmHg. This pressor response was 80% inhibited by intracerebroventricular (icv) injection of hemicholinium-3 (HC-3), 20 ug. Simultaneous icv injection of HC-3 and choline (365 ug) prevented the inhibition of the pressor response by HC-3. In spontaneously hypertensive rats, injection of HC-3 either icv (20 ug) or bilaterally into the posterior hypothalamic nuclei (1 ug) decreased SBP by about 40 mmHg. The effect of intrahypothalamic HC-3 was completely blocked by simultaneous injection of choline (24.3 ug) into the same site. The hypotensive effect of icv HC-3 was completely blocked by icv choline (243 ug) and was inhibited up to 60% by injections of choline (24.3 ug) into the posterior hypothalamic nuclei. 相似文献
95.
L M Dong T Yamamura S Tajima A Yamamoto 《Biochemical and biophysical research communications》1992,187(2):1180-1186
Apo E5(Glu3----Lys) is a naturally occurring apolipoprotein E (apo E) mutant found in patients with hyperlipoproteinemia and atherosclerosis. It has been shown to have a high affinity for low density lipoprotein (LDL) receptors. In this study, mutant apo E5 was produced by Chinese hamster ovary cells by means of an in vitro site-directed mutagenesis technique, and its LDL receptor binding activity was assessed. The apo E5 obtained from gene expression bound more readily to the LDL receptor than did plasma apo E3. The concentrations required for 50% competitive binding of 125I-labeled LDL to the LDL receptors were 58.9 ng/ml for plasma apo E3 and 25.7 ng/ml for the expressed apo E5. The expressed apo E5 displayed 229% normal binding. This result is highly consistent with that obtained with plasma apo E5, which showed 217% normal binding. Although the experimental apo E isoproteins contained more sialic acid than plasma apo E, the extent of sialylation had no effect on the receptor binding of apo E. 相似文献
96.
Summary The effect ofin situ extraction and elicitor treatment on shikonin production was studied with the suspension cultures ofLithospermum erythrorhizon. Shikonin concentration of 60 mg/L was achieved by the use of both techniques which was 24 times higher than that of control culture, and 65 times higher in terms of shikonin productivity. The host-pathogen effect of elicitor treatment andin situ extraction for product removal were effective for shikonin production. 相似文献
97.
Shu-Yu Yu Bao-Lin Mao Ping Xiao Wei-Ping Yu Ying-Long Wang Chang-Zhi Huang Wang-Qiu Chen Xiang-Zhan Xuan 《Biological trace element research》1990,24(2-3):105-108
This pilot study evaluated the feasibility and effectiveness of conducting a double-blind clinical trial for the prevention of lung cancer with selenium (Se) in Yunnan Tin Corporation, the People's Republic of China, where the incidence rates of lung cancer are extraordinarily high among the miners. Forty healthy miners were randomized to either 300 μg of Se in high Se malt cakes or an identical placebo of malt cakes daily for one year. Subjects consumed their usual daily diet. The low Se concentrations in plasma (0.05±0.008 μg/mL) and hair (0.442±0.085 μg/g) reflected their low dietary Se intake in the control subjects. In Se-supplemented group, the Se status was increased by 178% for serum and 194.8% for hair. The serum GSHpx activity was increased by 155.7%, whereas the lipid peroxide level was reduced by 74.5% compared to the placebo. The results of UDS assay indicated that the lymphocyte DNA damage induced by ultraviolet irradiation and carcinogen 3,4-benzpyrene could be protected by Se supplementation. Se-supplementation did not affect the liver function test (SGPT), as well as the concentrations of hemoglobin, albumin, and cholesterol. Thus, daily intake of 300 μg Se in form of Se-malt as a chemopreventive measure is safe and effective to humans with low Se status. 相似文献
98.
Biological Trace Element Research - The essential trace elements in 30 traditional Chinese medicines, (24 tonics and 6 nontonics) were determined by proton-induced X-ray emission. The... 相似文献
99.
本文研究了连香树科(Cercidiphyllaceae)叶的宏观结构,首次报道连香树齿腺体显微结构及晶体类型,并对叶柄维管束的变化作了进一步研究。通过与近缘科的比较,我们认为连香树科的系统演化处于孤立地位,和金缕梅科有较近的亲缘关系,与木兰科较为疏远。 相似文献
100.
安徽省达到芒属植物资源及其开发利用 总被引:9,自引:0,他引:9
安徽省芒属植物有3种量变种:五节芒(Miscanthus floridulus)、芒(M.sinensis var. sinensis)、紫芒(M. sinensis var. purpurascens)和荻(M. saccharifl-orus);其中紫芒为本省地理分布新记录。它们的资源量大;如全省仅连片五节芒草地即达260多万亩,一般亩产鲜草1000—2500公斤。芒属植物是利用价值很高的资源禾草:饲用价值“良等”;保持水土能力强;纤维品质优良,为制浆造纸及编织的上等原料;并可药用。最后,提出了开发利用芒属植物资源的几点建议。 相似文献