排序方式: 共有19条查询结果,搜索用时 265 毫秒
11.
Andrew Kinloch Verena Tatzer Robin Wait David Peston Karin Lundberg Phillipe Donatien David Moyes Peter C Taylor Patrick J Venables 《Arthritis research & therapy》2005,7(6):R1421
Antibodies against citrullinated proteins are highly specific for rheumatoid arthritis (RA), but little is understood about
their citrullinated target antigens. We have detected a candidate citrullinated protein by immunoblotting lysates of monocytic
and granulocytic HL-60 cells treated with peptidylarginine deiminase. In an initial screen of serum samples from four patients
with RA and one control, a protein of molecular mass 47 kDa from monocytic HL-60s reacted with sera from the patients, but
not with the serum from the control. Only the citrullinated form of the protein was recognised. The antigen was identified
by tandem mass spectrometry as α-enolase, and the positions of nine citrulline residues in the sequence were determined. Serum
samples from 52 patients with RA and 40 healthy controls were tested for presence of antibodies against citrullinated and
non-citrullinated α-enolase by immunoblotting of the purified antigens. Twenty-four sera from patients with RA (46%) reacted
with citrullinated α-enolase, of which seven (13%) also recognised the non-citrullinated protein. Six samples from the controls
(15%) reacted with both forms. α-Enolase was detected in the RA joint, where it co-localised with citrullinated proteins.
The presence of antibody together with expression of antigen within the joint implicates citrullinated α-enolase as a candidate
autoantigen that could drive the chronic inflammatory response in RA. 相似文献
12.
Debanjan Bhattacharya Vaibhavkumar S Gawali Laura Kallay Donatien K Toukam Abigail Koehler Peter Stambrook Daniel Pomeranz Krummel Soma Sengupta 《Experimental biology and medicine (Maywood, N.J.)》2021,246(19):2128
γ-aminobutyric acid or GABA is an amino acid that functionally acts as a neurotransmitter and is critical to neurotransmission. GABA is also a metabolite in the Krebs cycle. It is therefore unsurprising that GABA and its receptors are also present outside of the central nervous system, including in immune cells. This observation suggests that GABAergic signaling impacts events beyond brain function and possibly human health beyond neurological disorders. Indeed, GABA receptor subunits are expressed in pathological disease states, including in disparate cancers. The role that GABA and its receptors may play in cancer development and progression remains unclear. If, however, those cancers have functional GABA receptors that participate in GABAergic signaling, it raises an important question whether these signaling pathways might be targetable for therapeutic benefit. Herein we summarize the effects of modulating Type-A GABA receptor signaling in various cancers and highlight how Type-A GABA receptors could emerge as a novel therapeutic target in cancer. 相似文献
13.
Karidia Diallo Hugues Loemba Maureen Oliveira Donatien D. Mavoungoua Mark A. Wainberg 《Nucleosides, nucleotides & nucleic acids》2013,32(10-12):2019-2024
Abstract The inhibition of' HIV-I replication in vitro by Immunor 28 (IM28), an analog of dehydroepiandrosterone (DHEA), was monitored using the HIV- 1 laboratory wild-type strain IIIB. Evaluation of the 50% inhibitory dose (IC50) revealed a decrease in HIV-1 replication giving an IC, value around 22 μM. The toxicity of the drug has been determined also, in MT2 cells and PBMCs. 60 μM of IM28 provoked a 50% decrease in cell viability while DHEA caused the same decrease at 75 μM in MT2 cells. These values are 125 μM for IM28 in PBMCs and 135 μM for DIIBA. Thus, DHEA is less toxic than IM28, but IM28 has a higher antiviral activity. 相似文献
14.
Fructueux Modeste Amona David W. Denning Donatien Moukassa Michel Develoux Christophe Hennequin 《PLoS neglected tropical diseases》2021,15(5)
The Republic of Congo (RoC) is one of the African countries with the most histoplasmosis cases reported. This review summarizes the current status regarding epidemiology, diagnostic tools, and treatment of histoplasmosis in the RoC. A computerized search was performed from online databases Medline, PubMed, HINARI, and Google Scholar to collect literature on histoplasmosis in the RoC. We found 57 cases of histoplasmosis diagnosed between 1954 and 2019, corresponding to an incidence rate of 1–3 cases each year without significant impact of the AIDS epidemic in the country. Of the 57 cases, 54 (94.7%) were cases of Histoplasma capsulatum var. duboisii (Hcd) infection, African histoplasmosis. Three cases (5.3%) of Histoplasma capsulatum var. capsulatum infection were recorded, but all were acquired outside in the RoC. The patients’ ages ranged between 13 months to 60 years. An equal number of cases were observed in adults in the third or fourth decades (n = 14; 24.6%) and in children aged ≤15 years. Skin lesions (46.3%), lymph nodes (37%), and bone lesions (26%) were the most frequent clinical presentations. Most diagnoses were based on histopathology and distinctive large yeast forms seen in tissue. Amphotericin B (AmB) was first line therapy in 65% of the cases and itraconazole (25%) for maintenance therapy. The occurrence of African histoplasmosis in apparently normal children raises the possibility that African histoplasmosis is linked to environmental fungal exposure. 相似文献
15.
Teponno RB Tapondjou AL Gatsing D Djoukeng JD Abou-Mansour E Tabacchi R Tane P Stoekli-Evans H Lontsi D 《Phytochemistry》2006,67(17):1957-1963
Two clerodane diterpenoids, Bafoudiosbulbins A 1, and B 2, together with five known compounds: tetracosanoic acid, 1-(tetracosanoyl)-glycerol, trans-tetracosanylferulate, beta-sitosterol and 3-O-beta-D-glucopyranosyl-beta-sitosterol were isolated from the tubers of Dioscorea bulbifera L. var sativa. Their structures were established by spectroscopic methods (1D and 2D-NMR, MS) and X-ray crystallographic diffraction analysis of compound 1. The CH2Cl2-soluble portion of the crude extract and the two clerodanes were screened for anti-bacterial activity using both agar diffusion and broth dilution techniques against Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Salmonella typhi, Salmonella paratyphi A and Salmonella paratyphi B. They both showed significant activities against P. aeruginosa, S. typhi, S. paratyphi A and S. paratyphi B. 相似文献
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17.
Paolo Casale Gaspard Abitsi Marie Pierre Aboro Pierre Didier Agamboue Laureen Agbode Nontsé Lois Allela Davy Angueko Jean Noel Bibang Bi Nguema François Boussamba Floriane Cardiec Emmanuel Chartrain Claudio Ciofi Yves Armand Emane J. Michael Fay Brendan J. Godley Carmen Karen Kouerey Oliwiwina Jean de Dieu Lewembe Donatien Leyoko Georges Mba Asseko Pulcherie Mengue M’adzaba Jean Hervé Mve Beh Chiara Natali Clauvice Nyama-Mouketou Jacob Nzegoue Carole Ogandagas Richard J. Parnell Guy Anicet Rerambyath Micheline Schummer Gnandji Guy-Philippe Sounguet Manjula Tiwari Bas Verhage Raul Vilela Lee White Matthew J. Witt Angela Formia 《Biodiversity and Conservation》2017,26(10):2421-2433
Gabon hosts nesting grounds for several sea turtle species, including the world’s largest rookery for the leatherback turtle (Dermochelys coriacea), Africa’s largest rookery for the olive ridley turtle (Lepidochelys olivacea) and smaller aggregations of the hawksbill turtle (Eretmochelys imbricata) and green turtle (Chelonia mydas). To assess the level of incidental captures of turtles by the Gabonese trawl fishery, an onboard observer program was conducted in the period 2012–2013. A total of 143 turtles were captured by 15 trawlers during 271 fishing days. The olive ridley turtle was the main species captured (80% of bycaught turtles), with mostly adult-sized individuals. The remaining 20% included green turtles, hawksbill turtles, leatherback turtles and undetermined species. Bycatch per unit of effort (BPUE) of olive ridley turtles varied greatly depending on the period of the year (range of means: 0.261–2.270). Dead and comatose turtles were 6.2 and 24.6% respectively (n = 65). By applying the available fishing effort to two BPUE scenarios (excluding or considering a seasonal peak), the total annual number of captures was estimated as ranging between 1026 (CI 95% 746–1343) and 2581 (CI 95% 1641–3788) olive ridley turtles, with a mortality ranging from 63 (CI 95% 13–135) to 794 (CI 95% 415–1282) turtles per year depending on the scenario and on the fate of comatose turtles. Such a potential mortality may be reason for concern for the local breeding population of olive ridley turtles and recommendations in terms of possible conservation measures and further research are given. 相似文献
18.
François Donatien Ursenbacher Sylvain Boissinot Alexandre Ysnel Frédéric Lourdais Olivier 《Conservation Genetics》2021,22(5):823-837
Conservation Genetics - Human activities have a major impact on habitat connectivity and landscape structure. In this context, it is critical to better understand animal movements and gene flow to... 相似文献
19.
RNA virus populations will undergo processes of mutation and selection resulting in a mixed population of viral particles. High throughput sequencing of a viral population subsequently contains a mixed signal of the underlying clones. We would like to identify the underlying evolutionary structures. We utilize two sources of information to attempt this; within segment linkage information, and mutation prevalence. We demonstrate that clone haplotypes, their prevalence, and maximum parsimony reticulate evolutionary structures can be identified, although the solutions may not be unique, even for complete sets of information. This is applied to a chain of influenza infection, where we infer evolutionary structures, including reassortment, and demonstrate some of the difficulties of interpretation that arise from deep sequencing due to artifacts such as template switching during PCR amplification. 相似文献