全文获取类型
收费全文 | 707篇 |
免费 | 53篇 |
专业分类
760篇 |
出版年
2023年 | 4篇 |
2022年 | 5篇 |
2021年 | 6篇 |
2020年 | 9篇 |
2019年 | 11篇 |
2018年 | 15篇 |
2017年 | 12篇 |
2016年 | 17篇 |
2015年 | 26篇 |
2014年 | 33篇 |
2013年 | 52篇 |
2012年 | 69篇 |
2011年 | 43篇 |
2010年 | 31篇 |
2009年 | 31篇 |
2008年 | 43篇 |
2007年 | 40篇 |
2006年 | 39篇 |
2005年 | 47篇 |
2004年 | 43篇 |
2003年 | 35篇 |
2002年 | 26篇 |
2001年 | 10篇 |
2000年 | 5篇 |
1999年 | 10篇 |
1998年 | 9篇 |
1997年 | 7篇 |
1996年 | 2篇 |
1995年 | 7篇 |
1994年 | 8篇 |
1993年 | 5篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1990年 | 3篇 |
1989年 | 5篇 |
1988年 | 2篇 |
1987年 | 6篇 |
1986年 | 8篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 6篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1979年 | 3篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1970年 | 1篇 |
1967年 | 1篇 |
1966年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有760条查询结果,搜索用时 0 毫秒
731.
732.
733.
Manolo Casagrande Anna Barteselli Nicoletta Basilico Silvia Parapini Donatella Taramelli Anna Sparatore 《Bioorganic & medicinal chemistry》2012,20(19):5965-5979
With the aim to investigate the effect of different heterocyclic rings linked to the 4-aminoquinoline nucleus on the antimalarial activity, a set of 7-chloro-N-(heteroaryl)-methyl-4-aminoquinoline and 7-chloro-N-(heteroaryl)-4-aminoquinoline was synthesized and tested in vitro against D-10 (CQ-S) and W-2 (CQ-R) strains of Plasmodium falciparum. All compounds exhibited from moderate to high antiplasmodial activities. The activity was strongly influenced both by the presence of a methylenic group, as a spacer between the 4-aminoquinoline and the heterocyclic ring, and by the presence of a basic head. The most potent molecules inhibited the growth of both CQ-S and CQ-R strains of P. falciparum with IC50 < 30 nM and were not toxic against human endothelial cells. These results confirm that the presence of an heteroaryl moiety in the side chain of 7-chloro-4-aminoquinoline is useful for the design and development of new powerful antimalarial agents. 相似文献
734.
Di Valentin M Malorni D Maniero AL Agostini G Giacometti G Vianelli A Vannini C Cattaneo AG Brunel LC Carbonera D 《Photosynthesis research》2002,71(1-2):33-44
Chemical oxidation of the chlorosomes from Chloroflexus aurantiacus and Chlorobium tepidum green bacteria produces bacteriochlorophyll radicals, which are characterized by an anomalously narrow EPR signal compared to in vitro monomeric BChl c
.+ [Van Noort PI, Zhu Y, LoBrutto R and Blankenship RE (1997) Biophys J 72: 316–325]. We have performed oxidant concentration and temperature-dependent X-band EPR measurements in order to elucidate the line narrowing mechanism. The linewidth decreases as the oxidant concentration is increased only for Chloroflexus indicating that for this system Heisenberg spin exchange is at least partially responsible for the EPR spectra narrowing. For both species the linewidth is decreasing on increasing the temperature. This indicates that temperature-activated electron transfer is the main narrowing mechanism for BChl radicals in chlorosomes. The extent of the electron transfer process among different BChl molecules has been evaluated and a comparison between the two species representative of the two green bacteria families has been made. In parallel, high frequency EPR experiments have been performed on the oxidized chlorosomes of Chloroflexus and Chlorobium at 110 and 330 GHz in the full temperature range investigated at X-band. The g-tensor components obtained from the simulation of the 330 GHz EPR spectrum from Chlorobium show the same anisotropy as those of monomeric Chl a
.+ [Bratt PJ, Poluektov OG, Thurnauer MC, Krzystek J, Brunel LC, Schrier J, Hsiao YW, Zerner M and Angerhofer A (2000) J Phys Chem B 104: 6973–6977]. The spectrum of Chloroflexus has a nearly axial g-tensor with reduced anisotropy compared to Chlorobium and monomeric Chl a
in vitro. g-tensor values and temperature dependence of the linewidth have been discussed in terms of the differences in the local structure of the chlorosomes of the two families.This revised version was published online in October 2005 with corrections to the Cover Date. 相似文献
735.
Donatella Mares 《Plant biosystems》2013,147(3):323-334
Abstract The effects of either synthetic or natural substances on the normal morphogenetic processes in fungi are reviewed. Original results concerning the changes induced in the dermatophyte Microsporum cookei Ajello by the UVA-activated coumarin, herniarin, are reported. Alterations in the shape and wall assembly of parietal components are discussed on the basis of the most recent knowledge on the formation and growth of fungal hyphae. 相似文献
736.
Giuliana Allegrucci Adalgisa Caccone Donatella Cesaroni Valerio Sbordoni 《Journal of evolutionary biology》1992,5(1):121-148
In this paper we attempt to investigate relationships between the amount of genetic divergence in nuclear genes and the degree of morphological differentiation for different sets of characters in Dolichopoda cave crickets. Six populations representing five Dolichopoda species from Central and Southern Italy have been studied. The overall genetic divergence at nuclear genes was estimated both by single copy DNA-DNA hybridization and allozyme frequencies at 26 loci. Euclidean distances for two multivariate sets of morphometric variables: one describing body and appendage morphology, the other male epiphallus shape. Results showed a close agreement between the branching patterns of ΔTm values from DNA hybridization and Nei's allozyme distance values. On the other hand, patterns of morphological divergence revealed independent trends, although the branching pattern based on epiphallus morphology matched to some extent the phylogenies inferred from molecular data. The relative value of molecular and morphological characters as reliable phylogenetic tracers was evaluated in relation to their dependence on evolutionary factors. Implications of these findings on the calibration of molecular clocks are also discussed. The absolute rate of molecular change based on scDNA was estimated to be at least 0.98% divergence/my/lineage. This result is in agreement with calibrations attempted on other insects. Estimates of time of divergence based on allozymes (Nei's D) were highly consistent with the estimate from geological data. 相似文献
737.
738.
Dina Cocco Luisa Rossi Donatella Barra Francesco Bossa Giuseppe Rotilio 《FEBS letters》1982,150(2):303-306
Reaction with cyanate leads to a reversible change of the EPR spectrum of Cu,Zn-superoxide dismutase and to time-dependent carbamoylation of the lysine residues of the enzyme, producing a stable covalent derivative with more negative charge. The carbamoylated enzyme is less active than the native enzyme in spite of unaltered EPR spectra. The extent of this inactivation is much less when the enzyme activity is measured at low ionic strength. These results show that integrity of the active site is not the sole factor playing a role in the enzyme mechanism and that the ionic strength effect is related to electrostatic interactions between O−2 and surface charges of the protein. 相似文献
739.
Andrea Berti Elda Tremori Luigia Pazzagli Donatella Degl''Innocenti Guido Camici Gianni Cappugi Giampaolo Manao Giampietro Ramponi 《Journal of Protein Chemistry》1991,10(1):91-102
Acylphosphatase was purified from rat skeletal muscle essentially by gel filtration and high-performance ion-exchange chromatography. The complete amino acid sequence was reconstructed by using the sequence data obtained from tryptic, peptic, andS. aureus V8 protease peptides. The protein consists of 96 amino acid residues and is acetylated at the NH2-terminus. The immunological cross-reactivity of acylphosphatase from rat and horse skeletal muscle was examined by ELISA. The reaction with rabbit antiserum revealed the presence of at least five antigenic sites on rat enzyme, two of which are common to horse muscle enzyme. Anti-rat antibodies also recognize the peptide that corresponds to the initial part of the molecule, which varies greatly from equine enzyme. Two completely new antigenic sites are herein described: the first can be considered the main antigenic site and is located within positions 21–36, the second is in the COOH-terminal part of the molecule. A mixture of immunoreactive peptides gives strong antibody-antigen reaction inhibition (94%). 相似文献
740.
Rolando Oddone Donatella Barra Gino Amiconi Paolo Ascenzi Cataldo Tarricone Martino Bolognesi Fabrizio Bortolotti Enea Menegatti 《Journal of molecular recognition : JMR》1994,7(1):39-46
Values of the association equilibrium constant (Ka) for the binding of the native and of the cyanogen bromide-cleaved bovine basic pancreatic trypsin inhibitor (native BPTI and [Hse lactone-52]-52,53-seco-BPTI, respectively) to neuraminidase-treated porcine pancreatic β-Kallikrein-B (kallikrein) and bovine α-chymotrypsin (chymotrypsin) have been determined between pH4.0 and 9.0, and 20.0°C. Over the whole pH range explored, native BPTI and [Hse lactone-52]-52,53-seco-BPTI show the same affinity for kallikrein. On the other hand, the affinity of [se lactone-52]-52,53-seco-BPTI for chymotrypsin is high4er, around neutrality, than that found for native BPTI by about one order of magnitude, coverging in the acidic pH limb. The simplest mechanism accounting for the observed data implies that, on lowering the pH from 9.0 to 4.0 (i) the decrease in affinity for the binding of native BPTI to kalikrein and chymotrypsin, as well as for the association of [Hse lactone-52]-52,53-seco-BPTI to kalikrein, reflects the acidic pK shift, upon inhibitor association, of a single inozing group; and (ii) the decrease of Ka values for [Hse lactone-52]-52,53-seco-BPTI binding to chymotrypsin appears to be modulated by the acidic pK shift, upon inhibitor association, of two non-equivalent proton-binding residues. On the basis of the stereochemistry of the serine proteinase/inhibitor contact region(s), these data indicate that long-rang structural changes in [Hse lactone-52]-52,53-seco-BPTI are energetically linked to the chymotrypsin: inhibitor complex formation. This observation represents an important aspect for the mechanism of molecular recognition and regulation in BPTI. 相似文献