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181.
Donald H. Williams 《CMAJ》1967,96(14):1040-1044
Five basic forms of continuing educational endeavour by physicians are listed in rank order. These components constitute an indivisible unit bound together by self-learning. The scholarly habit of planned daily reading and study in a home library-sanctuary as an integral part of a physician''s workday heads the list. Day-to-day informal and formal colleague-association in patient care in the community and teaching hospital, in group practice and by consultation is the present major form of continuing educational endeavour. Emphasized is the sabbatical return every three to five years for three months at least to the teaching hospital to reinforce scholarly motivation and attitudes and to acquire new skills and knowledge. Attendance at scientific sessions of learned professional societies and short courses should be accompanied by presession and post-session guided reading to be undertaken in the physician''s home library-sanctuary. 相似文献
182.
Electron microscopy was used to study pollen wall ontogeny in Zea mays. The initial stage of development consisted of compartmentalization of microspores within callose special walls. Microspore plasma membranes retracted and tubular elements of the endoplasmic reticulum became perpendicularly oriented to the plasma membranes. Evaginations of the endoplasmic reticulum into the microspore plasma membrane resulted in the establishment of a template or blueprint of the mature pollen wall. Sporopollenin deposition upon the template began immediately after dissolution of the callose special walls and release of the microspores into the anther locule. The columellae were the first pollen wall units to be formed; the tectum and foot layer became established shortly thereafter. The granular endexine was the last-formed unit. The relationships of membrane systems to the ontogeny of the pollen wall units and the mode of pollen wall growth are discussed. 相似文献
183.
In order to ascertain the prevalence of chronic respiratory disease in residents of a rural town and to determine the relative importance of tobacco smoking and air pollution, a survey was conducted of 726 persons living at Chilliwack, British Columbia, in May and June, 1963. Over 95% of a random sample of adults was interviewed and performed simple tests of respiratory function. The sample was selected from a commercial census. An analysis of the demographic characteristics of the sample indicated that the group, aged 25 to 74 years, was reasonably representative for detailed study of chronic respiratory disease. 相似文献
184.
The distribution of the ABO, A1, D, E, C, and Kell blood types in relation to selected psychiatric diagnoses was studied in over 700 consecutive admissions to a provincial mental hospital. Predicted relationships based upon recent work in other laboratories were supported by consistent trends approaching statistical significance. In addition, blood group O was found to be significantly associated with involutional melancholia. 相似文献
185.
186.
Donald G. Musson Alison M. Bidgood Orest Olejnik 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1991,565(1-2)
Prednisolone, prednisolone acetate and prednisolone sodium phosphate are glucocorticoids used for ocular, anti-inflammatory therapy. A reversed-phase high-performance liquid chromatographic assay using ultraviolet detection has been developed that affords baseline resolution of the above analytes in balanced salt solutions and rabbit aqueous humor. The drugs can be quantified at 0.025–0.05 μg/ml in the above matrices; 6α-methylprednisolone is used as the internal standard. Both esters of prednisolone are vulnerable to chemical and enzymatic hydrolysis giving prednisolone. Analysis of aqueous humor samples shows prednisolone acetate penetrating/metabolizing primarly to prednisolone; prednisolone sodium phosphate penetrates the cornea giving the ester and alcohol. 相似文献
187.
An unusual Pleistocene patch reef is exposed in a coastal cliff at Grotto Beach, San Salvador, Bahamas. The reef is a coralline framestone constructed mainly by Porites astreoides together with a few large heads of Diploria strigosa and Montastrea annularis, and is capped by a dense thicket of Neogoniolithon strictum that is interpreted as marking the subtidal/intertidal boundary. The reef is flanked to the northeast by laminated to low-angle cross-laminated intraclastic grainstones and to the southwest by skeletal rudstone of reefal and interreefal derivation. Uranium-series dating of pure aragonite from a Diploria corallum yielded an age of 123 000±9000 years. Reef growth began on an erosional surface underlain by steeply crossbedded eolian grainstone. As the reef grew upward, it also grew laterally over adjacent penecontemporaneous subtidal sediments. The reef was eventually buried by 2.3 m of shallow subtidal and beach sediments that apparently prograded seaward during a highstand, or possibly while sea level was still rising. The shallow subtidal sediments are mainly peloidal, ooidal and skeletal grainstones that are pervasively bioturbated. The overlying beach facies comprises predominantly laminated, sparsely burrowed grainstone. The beach and shallow subtidal facies contain boulders of fine-grained laminated grainstone that are interpreted as storm-tossed blocks of beachrock. Living analogs of the Grotto Beach fossil reef lie off East Beach, San Salvador. Several of these have a flourishing cap of Neogoniolithon that extends above low-tide level and we believe that the Neogoniolithon cap of Grotto Beach reef did likewise. Wherever found in the stratigraphic record this facies should serve to identify the subtidal/intertidal boundary. The uppermost Pleistocene beach sediments associated with Grotto Beach fossil reef lie 5.8 m above present-day mean sea level, which ist strong evidence that this portion of San Salvador has undergone little subsidence since the Grotto Beach section was deposited. 相似文献
188.
Daniel W. Bean Donald L. Silhacek 《Archives of insect biochemistry and physiology》1989,10(4):333-348
The levels of an 81K storage protein in the waxmoth, Galleria mellonella, were monitored during the course of development using rocket immunoelectrophoresis. During the fifth and sixth larval stadia, 81K protein levels increased during feeding and growth but sharply declined at each larval molt. During the fifth and sixth stadia hemolymph levels of the 81K protein increased to about 1 and 2.5 mg/ml, respectively, with no discernible differences between levels in males and females. Neither the fat body nor the remainder of the carcass contained the 81K protein, indicating that the accumulation of this protein during the intermolt period was exclusively in the hemolymph and redistribution of the 81K protein into other tissues does not occur at the final two larval molts. During the seventh (final) larval stadium the absolute quantities of the 81K protein increased from 23 μg per insect to over 1,600 μg in females and to 300 μg in males. The hemolymph concentration of the 81K protein reached 28 mg/ml in females and 6 mg/ml in males with only low levels found in the remaining tissues. Shortly after pupal apolysis, marked by eyespot retraction, the fat body in both sexes rapidly and quantitatively sequestered the 81K protein from the hemolymph. The 81K protein in the hemolymph of both males and females rapidly dropped to nearly zero concentration by pupation. The 81K storage protein remained localized in the fat body cells after uptake occurred, even though the fat body cells disaggregate and reaggregate during metamorphosis. During pharate adult development the 81K storage protein disappeared from the fat body without entering the hemolymph. At adult eclosion 81K was virtually absent from the tissues of both males and females. 相似文献
189.
Donald T. Wigle 《CMAJ》1989,140(6):671-673
190.
Donald F. Kapraun 《Journal of applied phycology》1989,1(3):239-246
Newly released zoospores fromEnteromorpha linza (L.) J. Ag. lack significant cellulose cell wall material and are suitable for treatment as protoplasts in a parasexual fusion process using high pH-Ca+ +, PEG and centrifugation. Treated zoospores settled on glass cover slips within 3 h and were examined microscopically at 1000 ×. Presumptive fusion products were identified by their larger size and presence of twin chloroplasts and eyespots. Unfused zoospores adjacent to fusion cells were killed by 2–3 min exposure to blue light (410–490 nm) from a high pressure mercury illuminator. Unexposed fusion cells developed into uniseriate germlings within 10 days at which stage they could be readily identified at 60 × with a dissecting microscope and isolated by micropipette. Ten-day germlings from both unfused zoospores and fusion cells were stained with the DNA-localizing fluorochrome hydroethidine and relative nuclear DNA content determined with epi-(incident) UV illumination. All germlings were found to be uninucleate. Germlings from unfused zoospores had haploid nuclei with 1N = 10 and 1C and 2C levels of DNA, while germlings from fusion cells had diploid nuclei with 2N = 20 and 2C and 4C levels of DNA. These result are interpreted as evidence of karyogamy following parasexual zoospore fusions. Isolated diploid germlings, cultured for 10 weeks were found to conserve their 2N chromosome complements and elevated levels of nuclear DNA. Although most diploid germlings were morphologically similar to haploid control plants, some exhibited ‘gigas’ characteristics, including larger cells, chloroplasts, and nuclei. These results are discussed in terms of unique phenotypes that result when nuclear and organellar genes are combined in different ways. 相似文献