全文获取类型
收费全文 | 767篇 |
免费 | 103篇 |
出版年
2023年 | 4篇 |
2022年 | 6篇 |
2021年 | 16篇 |
2020年 | 10篇 |
2019年 | 10篇 |
2018年 | 20篇 |
2017年 | 10篇 |
2016年 | 30篇 |
2015年 | 39篇 |
2014年 | 41篇 |
2013年 | 51篇 |
2012年 | 47篇 |
2011年 | 58篇 |
2010年 | 31篇 |
2009年 | 28篇 |
2008年 | 32篇 |
2007年 | 33篇 |
2006年 | 22篇 |
2005年 | 32篇 |
2004年 | 23篇 |
2003年 | 25篇 |
2002年 | 31篇 |
2001年 | 21篇 |
2000年 | 21篇 |
1999年 | 18篇 |
1998年 | 15篇 |
1997年 | 11篇 |
1996年 | 12篇 |
1995年 | 14篇 |
1994年 | 11篇 |
1993年 | 11篇 |
1992年 | 16篇 |
1991年 | 7篇 |
1990年 | 11篇 |
1989年 | 11篇 |
1988年 | 15篇 |
1987年 | 8篇 |
1986年 | 6篇 |
1985年 | 6篇 |
1984年 | 5篇 |
1983年 | 8篇 |
1982年 | 7篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1977年 | 4篇 |
1974年 | 3篇 |
1970年 | 2篇 |
1969年 | 2篇 |
1965年 | 2篇 |
1964年 | 2篇 |
排序方式: 共有870条查询结果,搜索用时 31 毫秒
41.
Hybridization in large-bodied New World primates 总被引:2,自引:0,他引:2
Cortés-Ortiz L Duda TF Canales-Espinosa D García-Orduña F Rodríguez-Luna E Bermingham E 《Genetics》2007,176(4):2421-2425
Well-documented cases of natural hybridization among primates are not common. In New World primates, natural hybridization has been reported only for small-bodied species, but no genotypic data have ever been gathered that confirm these reports. Here we present genetic evidence of hybridization of two large-bodied species of neotropical primates that diverged approximately 3 MYA. We used species-diagnostic mitochondrial and microsatellite loci and the Y chromosome Sry gene to determine the hybrid status of 36 individuals collected from an area of sympatry in Tabasco, Mexico. Thirteen individuals were hybrids. We show that hybridization and subsequent backcrosses are directionally biased and that the only likely cross between parental species produces fertile hybrid females, but fails to produce viable or fertile males. This system can be used as a model to study gene interchange between primate species that have not achieved complete reproductive isolation. 相似文献
42.
David P. Belesky Jonathan J. Halvorson Joyce M. Ruckle Dariusz P. Malinowski Domingo J. Mata‐Padrino 《The Annals of applied biology》2019,175(1):42-53
Pastures in the Appalachian region of the United States comprise a mix of grasses, legumes and forbs that tend to differ in productivity within and among years. A high degree of spatial variability in hill‐land pasture creates microsite conditions that influence botanical composition of pasture. The variation in sward composition presents logistical challenges to livestock producers who rely on a dependable supply of herbage mass and nutritive value to meet production goals. Our objective was to determine if forage communities sown for specific functions, for example, superior dry matter productivity, resource patch exploitation or targeted seasonal production, adapted to changing growing conditions within and among years. Productivity of communities differed among years reflecting the cumulative influences of time, ontogenetic and environmental variations. Maximum productivity was influenced by the specific forage community and less so by simple clipping and fertiliser management. Naturalised swards clipped to emulate hay management tended to have sustained herbage productivity but lower nutritive value when compared to sown communities. Rankings of dry matter productivity of communities were similar for each year where bioactive composition, high productivity and warm season tended to produce the most, and stoloniferous‐rhizomatous and naturalised pasture the least. Regardless of initial sward composition, effective number of species as an index of diversity increased when frequently clipped swards were not fertilised, and when infrequently clipped swards were fertilised. Dry matter production patterns were not influenced by the effective number of species in any forage community suggesting that key species sustained productivity with volunteer species making lesser contribution to total productivity. The species composition of forage plant communities appears to be more important than clipping or fertiliser management practices as a means to sustain forage productivity. 相似文献
43.
Cortés-Ortiz Liliana Baiz Marcella D. Hermida-Lagunes Javier García-Orduña Francisco Rangel-Negrín Ariadna Kitchen Dawn M. Bergman Thore J. Dias Pedro A. D. Canales-Espinosa Domingo 《International journal of primatology》2019,40(1):114-131
International Journal of Primatology - Interspecific hybridization allows the introgression or movement of alleles from one genome to another. While some genomic regions freely exchange alleles... 相似文献
44.
Hydrogen is a potential sustainable energy source and it could become an alternative to fossil fuel combustion, thus helping to reduce greenhouse gas emissions. The biological production of hydrogen, instead of its chemical synthesis, is a promising possibility since this process requires less energy and is more sustainable and eco-friendly. Several microorganisms have been used for this purpose, but Escherichia coli is one of the most widely used in this field. The literature in this area has increased exponentially in the last 10 years and several strategies have been reported in an effort to improve hydrogen production. In this work, the stay of the art of hydrogen biosynthesis by E. coli and metabolic engineering strategies to enhance hydrogen production are reviewed. This work includes a discussion about the hydrogenase complexes responsible for the hydrogen synthesis in this microorganism and the central carbon metabolism pathways connected to this process. The main metabolic engineering strategies applied are discussed, including heterologous gene expression, adaptive evolution and metabolic and protein engineering. On the other hand, culture conditions, including the use of carbon sources such as glycerol, glucose or organic wastes, have also been considered. Yields and productivities of the most relevant engineered strains reported using several carbon sources are also compared. 相似文献
45.
Enrico Ceccarelli Ariadna Rangel Negrín Alejandro Coyohua-Fuentes Domingo Canales-Espinosa Pedro Américo D. Dias 《American journal of primatology》2020,82(2):e23099
Benefits of group life depend in large part on whether animals remain cohesive, which often requires collective decisions about where and when to move. During a group movement, the leader may be considered as the individual occupying the vanguard position of the group progression, when its movement evokes following by other group members. In nondespotic societies, individuals with greater incentives to move frequently are leaders. During 15 months of observations (1,712 contact hours), we investigated two mantled howler monkey (Alouatta palliata) groups at La Flor de Catemaco (Los Tuxtlas, Mexico) to examine whether sex and female reproductive state influenced leadership likelihood in two contexts: movements toward feeding trees; movements associated with loud calls, a group-defense behavior used by males of this genus. Females led and occupied forward positions during group movements toward feeding trees more often than adult males. Adult females led these movements more frequently when they were gestating than when they were lactating or cycling. There were no differences between sexes in the leadership of group movements associated with loud calls. Leadership by gestating females is perhaps the result of their higher nutritional/energetic needs when compared with cycling females, and of their greater mobility when compared with lactating females carrying dependent offspring. Female leadership during movements toward feeding trees may be a mechanism to optimize access to food resources in mantled howler monkeys. 相似文献
46.
Yuste E Sánchez-Palomino S Casado C Domingo E López-Galíndez C 《Journal of virology》1999,73(4):2745-2751
Muller's ratchet predicts fitness losses in small populations of asexual organisms because of the irreversible accumulation of deleterious mutations and genetic drift. This effect should be enhanced if population bottlenecks intervene and fixation of mutations is not compensated by recombination. To study whether Muller's ratchet could operate in a retrovirus, 10 biological clones were derived from a human immunodeficiency virus type 1 (HIV-1) field isolate by MT-4 plaque assay. Each clone was subjected to 15 plaque-to-plaque passages. Surprisingly, genetic deterioration of viral clones was very drastic, and only 4 of the 10 initial clones were able to produce viable progeny after the serial plaque transfers. Two of the initial clones stopped forming plaques at passage 7, two others stopped at passage 13, and only four of the remaining six clones yielded infectious virus. Of these four, three displayed important fitness losses. Thus, despite virions carrying two copies of genomic RNA and the system displaying frequent recombination, HIV-1 manifested a drastic fitness loss as a result of an accentuation of Muller's ratchet effect. 相似文献
47.
Hernández M García-Moro C Toja DI Esparza M González-José R 《Collegium antropologicum》2004,28(2):577-583
The seasonality of twinning in the Spanish populations has not been studied until now. Differences between seasonal distribution of the twin conceptions and those of the single births have been observed in other populations. The aim of this work is to explore the frequency of twinning in a rural population from Catalonia during the nineteenth century, as well as the seasonality patterns characterizing each of the twinning types. Data corresponding to all births recorded at Tortosa (South Catalonia) from 1801 to 1900 have been analyzed in order to study the twinning distribution. The distribution of the moving averages of the monthly rates of twins shows a peak in autumn. Twinning distribution differs from the total births' distribution in Tortosa. This fact is very clear in the case of unlike-sexed twins that have their greater incidence in the last quarter of the year, while the total maternities have their peak in the first one. 相似文献
48.
Bellés M Albina ML Sanchez DJ Corbella J Domingo JL 《Biological trace element research》2001,79(1):67-81
The present study was conducted to assess in rats the effects of oral aluminum (Al) exposure on calcium (Ca), magnesium (Mg),
manganese (Mn), copper (Cu), zinc (Zn), and iron (Fe) accumulation and urinary excretion. Three groups of plug-positive Sprague-Dawley
(SD) rats were given by gavage 0, 200, and 400 mg/kg/d of Al(OH)3 on gestational days 1–20. Three groups of nonpregnant female SD rats of the same age received Al(OH)3 by gavage at the same doses for 20 consecutive days. At the end of the treatment period, 24-h urine samples were collected
for analysis of Al and essential elements. Subsequently, all animals were sacrificed and samples of liver, bone, spleen, kidneys,
and brain were removed for metal analyses. With some exceptions, the urinary amounts of Al, Mn, and Cu excreted by pregnant
animals as well as the urinary levels of Al excreted by nonpregnant rats were higher in the Al-treated groups than in the
respective control groups. Although higher Al levels were found in the liver of pregnant rats, the concentrations of Al in
the brain of these animals were lower than those found in the same tissues of nonpregnant rats. With regard to the essential
elements, tissue accumulation was most affected in pregnant than in nonpregnant animals. In pregnant rats, the hepatic and
renal concentrations of Ca, Mg, Mn, Cu, Zn, and Fe, as well as the levels of Ca in bone, and the concentrations of Cu in brain
were significantly higher in the Al-exposed groups than in the control group. According to the current results, oral Al exposure
during pregnancy can produce significant changes in the tissue distribution of a number of essential elements. 相似文献
49.
Differential expression of cell-wall-related genes during the formation of tracheary elements in the Zinnia mesophyll cell system 总被引:7,自引:0,他引:7
Milioni D Sado PE Stacey NJ Domingo C Roberts K McCann MC 《Plant molecular biology》2001,47(1-2):221-238
Plants, animals and some fungi undergo processes of cell specialization such that specific groups of cells are adapted to carry out particular functions. One of the more remarkable examples of cellular development in higher plants is the formation of water-conducting cells that are capable of supporting a column of water from the roots to tens of metres in the air for some trees. The Zinnia mesophyll cell system is a remarkable tool with which to study this entire developmental pathway in vitro. We have recently applied an RNA fingerprinting technology, to allow the detection of DNA fragments derived from RNA using cDNA synthesis and subsequent PCR-amplified fragment length polymorphisms (cDNA-AFLP), to systematically characterize hundreds of the genes involved in the process of tracheary element formation. Building hoops of secondary wall material is the key structural event in forming functional tracheary elements and we have identified over 50 partial sequences related to cell walls out of 600 differentially expressed cDNA fragments. The Zinnia system is an engine of gene discovery which is allowing us to identify and characterize candidate genes involved in cell wall biosynthesis and assembly. 相似文献
50.
Cristina Escarmís Gema Gómez-Mariano Mercedes Dávila Ester Lázaro Esteban Domingo 《Journal of molecular biology》2002,315(4):647-661
Plaque-to-plaque transfers of RNA viruses lead to accumulation of mutations and fitness decrease. To test whether continuing plaque-to-plaque transfers would lead to viral extinction, we have subjected several low fitness foot-and-mouth disease virus (FMDV) clones to up to 130 successive plaque transfers, and have analyzed the evolution of plaque titers and genomic nucleotide sequences. No case of viral extinction could be documented. Some low fitness clones that posses an internal poly(A) tract evaded extinction by modifying the length or base composition of the poly(A) tract. The comparison of entire genomic sequences of FMDV clones at increasing plaque transfer number revealed that mutations accumulated at a uniform rate, and that they were distributed unevenly along the genome. Clusters of mutations were identified at different genomic sites in two plaque transfer lineages. Mutation clustering appears to occur stochastically and could not be related to fixation of compensatory mutations. The results document resistance of viral clones to extinction, and suggest that mutation clustering may be a mechanism of genetic diversification of low fitness virus. 相似文献