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101.
Henderson HL Hodson DJ Gregory SJ Townsend J Tortonese DJ 《Biology of reproduction》2008,78(2):370-377
Previous studies have provided evidence for a paracrine interaction between pituitary gonadotrophs and lactotrophs. Here, we show that GnRH is able to stimulate prolactin (PRL) release in ovine primary pituitary cultures. This effect was observed during the breeding season (BS), but not during the nonbreeding season (NBS), and was abolished by the application of bromocriptine, a specific dopamine agonist. Interestingly, GnRH gained the ability to stimulate PRL release in NBS cultures following treatment with bromocriptine. In contrast, thyrotropin-releasing hormone, a potent secretagogue of PRL, stimulated PRL release during both the BS and NBS and significantly enhanced the PRL response to GnRH during the BS. These results provide evidence for a photoperiodically modulated functional interaction between the GnRH/gonadotropic and prolactin axes in the pituitary gland of a short day breeder. Moreover, the stimulation of PRL release by GnRH was shown not to be mediated by the gonadotropins, since immunocytochemical, Western blotting, and PCR studies failed to detect pituitary LH or FSH receptor protein and mRNA expressions. Similarly, no gonadotropin receptor expression was observed in the pituitary gland of the horse, a long day breeder. In contrast, S100 protein, a marker of folliculostellate cells, which are known to participate in paracrine mechanisms within this tissue, was detected throughout the pituitaries of both these seasonal breeders. Therefore, an alternative gonadotroph secretory product, a direct effect of GnRH on the lactotroph, or another cell type, such as the folliculostellate cell, may be involved in the PRL response to GnRH in these species. 相似文献
102.
103.
Regulation of photosynthesis through source: sink imbalance in citrus is mediated by carbohydrate content in leaves 总被引:15,自引:0,他引:15
Domingo J. Iglesias Ignacio Lliso Francisco R. Tadeo Manuel Talon 《Physiologia plantarum》2002,116(4):563-572
In citrus, the occurrence of a sink effect on photosynthesis (A) is controversial. Leaf carbohydrates and photosynthetic rates in field-grown trees of Satsuma mandarin ( Citrus unshiu [Mak.] Marc.) cv. Okitsu, were measured to elucidate whether or not the demand for photoassimilates regulates A. The data indicated that the source-sink imbalances induced by different treatments altered both soluble (sucrose, glucose and fructose) and insoluble carbohydrates in leaves, as well as photosynthetic rates. In general, girdling and defruiting increased starch and reduced photosynthesis, whereas source-limiting conditions imposed through partial defoliations had the opposite effect. These results are compatible with the assumption that a lack of sink activity leads to carbohydrate accumulation and feedback inhibition of A, and vice versa. Further evidence supporting a source-sink effect on A was provided by measurements of the dry matter:leaf area ratio, since defoliations, for example, increased this ratio. The in vivo sucrose supplementation to plants with different source:sink ratios (control, defoliated, girdled and defruited plants) increased carbohydrates and reduced photosynthesis. This suggests that sugars may have, per se, the potential to repress photosynthetic rates in intact plants with active sinks. Based on these results we propose that sugar accumulation in citrus leaves causes a feedback inhibition of A. 相似文献
104.
J Villén E Borràs W M Schaaper R H Meloen M Dávila E Domingo E Giralt D Andreu 《Biologicals》2001,29(3-4):265-269
Functional reproduction of discontinuous antigenic site D of foot-and-mouth disease virus (FMDV) has been achieved by means of synthetic peptide constructions that integrate into a single molecule each of the three protein loops that define the antigenic site. The site D mimics are designed on the basis of the X-ray structure of FMDV type C-S8c1 with the aid of molecular dynamics, so that the five residues assumed to be involved in antigenic recognition are located on the same face of the molecule, exposed to solvent and defining a set of native-like distances and angles. The designed site D mimics are disulphide-linked heterodimers that consist of a larger unit containing VP2(71-84), followed by a polyproline module and by VP3(52-62), and a smaller unit corresponding to VP1(188-194). Guinea pig antisera to the peptides recognize the viral particle and compete with site D-specific monoclonal antibodies, while inoculation with a simple (non-covalently bound) admixture of the three VP1-VP3 sequences yields no detectable virus-specific serum conversion. Similar results have been reproduced in two cattle. Antisera to the peptides are also moderately neutralizing of FMDV in cell culture and partially protective of guinea pigs against challenge with the virus. These results demonstrate functional mimicry of the discontinuous site D by the peptides, which are therefore obvious candidates for a multicomponent peptide-based vaccine against FMDV. 相似文献
105.
106.
G Fischer M Domingo D Lodder N Katz M Reinacher E Eigenbrodt 《Virchows Archiv. B, Cell pathology including molecular pathology》1987,53(6):359-364
Preneoplastic liver lesions were produced in female Wistar rats by application of 25 mg/kg N-nitrosomorpholine (NNM), 14 mg/kg diethylnitrosamine (DENA), 0.075 mg/kg aflatoxin B1 (AFB1) or 160 mg/kg safrole. These carcinogens were administered in two equal doses 12 and 24 h after partial hepatectomy. The animals then received sodium phenobarbital (0.1% in tap water) for up to 410 days. Numerous altered hepatic foci (AHF) and hyperplastic nodules (HN) were detected enzyme histochemically by their negative ATPase reaction after application of AFB1, DENA and NNM; some AHF and HN were also caused by the weak carcinogen safrole. Immunohistochemically these lesions were also L-pyruvate kinase (L-PK)-negative with a high coincidence with regard to their number and area. These results confirm the role of L-PK, an enzyme affecting the pentose phosphate pathway, as a negative marker of preneoplastic liver lesions. 相似文献
107.
Ultramicroscopic study of the bile lipoprotein complex 总被引:2,自引:0,他引:2
108.
Esteban Domingo 《Journal of virology》2002,76(1):463-465
109.
110.
The apoprotein fraction of the bile lipoprotein complex: isolation, partial characterization and phospholipid binding properties 总被引:4,自引:0,他引:4
G Nalbone H Lafont J L Vigne N Domingo D Lairon C Chabert P Lechene J C Hauton 《Biochimie》1979,61(9):1029-1041
A bile apoprotein fraction (Apo BLC) was isolated by preparative isoelectric focusing (I.E.F.) from the detergent-free form of the bile lipoprotein complex (BLC). Analytical I.E.F. of Apo BLC yields a characteristic and reproducible pattern of two narrow acidic bands (pI 4,8-5,0). This apoprotein presents a strong tendency to undergo self-aggregation in aqueous buffer. A low molecular weight constituent of Apo BLC has been isolated after gel filtration, its mean Mw is estimated by SDS-PAGE at 7,500 daltons. The binding capacity of Apo BLC for phospholipids was investigated on dimyristoylphosphatidylcholine liposomes by gel filtration and zone electrophoresis. The resulting structures, larger than the original single-shelled vesicles, acquire and anodic electrophoretic mobility. Apo BLC has a weaker affinity for lysophosphatidylcholines: these phospholipids decrease the degree of aggregation of the apoprotein. These studies contribute additional data concerning the high affinity of Apo BLC for phosphatidylcholines, which are the major phospholipid constituents of bile. The discussion deals with the fact that association of Apo BLC with bile phosphatidylcholines may present some implications in the pathogeny of LpX and in the process of intestinal fat absorption. 相似文献