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排序方式: 共有333条查询结果,搜索用时 15 毫秒
71.
G. Musumeci P. Castrogiovanni V. Mazzone M. A. Szychlinska S. Castorina C. Loreto 《European journal of histochemistry : EJH》2014,58(2)
In this review article, we describe benefits and disadvantages of the established histochemical methods for studying articular cartilage tissue under normal, pathological and experimental conditions. We illustrate the current knowledge on cartilage tissue based on histological and immunohistochemical aspects, and in conclusion we provide a short overview on the degeneration of cartilage, such as osteoarthritis. Adult articular cartilage has low capacity to repair itself, and thus even minor injuries may lead to progressive damage and osteoarthritic joint degeneration, resulting in significant pain and disability. Numerous efforts have been made to implement the knowledge in the study of cartilage in the last years, and histochemistry proved to be an especially powerful tool to this aim. 相似文献
72.
Domenica Farci Patrycja Haniewicz Daniele de Sanctis Luca Iesu Sami Kereïche Mathias Winterhalter Dario Piano 《The Journal of biological chemistry》2022,298(6)
The radiation-resistant bacterium Deinococcus radiodurans is known as the world’s toughest bacterium. The S-layer of D. radiodurans, consisting of several proteins on the surface of the cellular envelope and intimately associated with the outer membrane, has therefore been useful as a model for structural and functional studies. Its main proteinaceous unit, the S-layer deinoxanthin-binding complex (SDBC), is a hetero-oligomeric assembly known to contribute to the resistance against environmental stress and have porin functional features; however, its precise structure is unknown. Here, we resolved the structure of the SDBC at ∼2.5 Å resolution by cryo-EM and assigned the sequence of its main subunit, the protein DR_2577. This structure is characterized by a pore region, a massive β-barrel organization, a stalk region consisting of a trimeric coiled coil, and a collar region at the base of the stalk. We show that each monomer binds three Cu ions and one Fe ion and retains one deinoxanthin molecule and two phosphoglycolipids, all exclusive to D. radiodurans. Finally, electrophysiological characterization of the SDBC shows that it exhibits transport properties with several amino acids. Taken together, these results highlight the SDBC as a robust structure displaying both protection and sieving functions that facilitates exchanges with the environment. 相似文献
73.
Giovanni Duro M. Beatrice Musumeci Paolo Colomba Carmela Zizzo Giuseppe Albeggiani Vittoria Mastromarino Massimo Volpe Camillo Autore 《Gene》2014
Fabry disease (FD) is a hereditary metabolic disorder caused by the partial or total inactivation of α-galactosidase A (α-gal A), a lysosomal hydrolase. This inactivation is responsible for the accumulation of undegraded glycosphingolipids in the lysosomes with subsequent cellular and microvascular dysfunction. Fabry is considered a rare disease, with an incidence of 1:40,000; however, there are good reasons to believe that it is often seen but rarely diagnosed. To date, more than 600 mutations have been identified in human GLA gene that are responsible for FD. 相似文献
74.
Alessandra Bitto Natasha Irrera Gabriele Pizzino Giovanni Pallio Federica Mannino Mario Vaccaro Vincenzo Arcoraci Federica Aliquò Letteria Minutoli Michele R. Colonna Maria Rosaria Galeano Michael Brines Chiara De Ponte Massimo Collino Francesco Squadrito Domenica Altavilla 《生物化学与生物物理学报:疾病的分子基础》2018,1864(2):632-639
Diabetes is characterized by poor wound healing which currently lacks an efficacious treatment. The innate repair receptor (IRR) is a master regulator of tissue protection and repair which is expressed as a response injury or metabolic stress, including in diabetes. Activation of the IRR might provide benefit for diabetic wound healing. A specific IRR agonist cibinetide was administered in an incisional wound healing model performed mice with genetic diabetes (db+/db+) and compared to the normal wild-type. Animals were treated daily with cibinetide (30 μg/kg/s.c.) or vehicle and euthanized 3, 7, and 14 days after the injury to quantitate vascular endothelial growth factor (VEGF), malondialdehyde (MAL), phospho-Akt (pAkt), phospho e-NOS (p-eNOS), and nitrite/nitrate content within the wound. Additional evaluations included quantification of skin histological change, angiogenesis, scar strength, and time to complete wound closure. Throughout the wound healing process diabetic animals treated with vehicle exhibited increased wound MAL with reduced VEGF, pAkt, peNOS and nitrite/nitrate, all associated with poor re-epitheliziation, angiogenesis, and wound breaking strength. Cibenitide administration significantly improved these abnormalities. The results suggest that cibinetide-mediated IRR activation may represent an interesting strategy to treat diabetes-associated wound healing. 相似文献
75.
Gabriele Giacomo Schiattarella Fabio Cattaneo Gianluigi Pironti Fabio Magliulo Giuseppe Carotenuto Marinella Pirozzi Roman Polishchuk Domenica Borzacchiello Roberta Paolillo Marco Oliveti Nicola Boccella Marisa Avvedimento Maria Sepe Giuseppe Gargiulo Assunta Lombardi Rosa Anna Busiello Bruno Trimarco Giovanni Esposito Antonio Feliciello Cinzia Perrino 《PloS one》2016,11(7)
76.
Luca Lanzanò Li Sui Evelina Costanzo Marisa Gulino Agata Scordino Salvatore Tudisco Francesco Musumeci 《Luminescence》2009,24(6):409-415
Delayed luminescence from a single dry soybean seed was investigated in both spectral and time domains, under different excitation wavelengths. Emission spectra were collected, under 337 nm laser excitation, from native and artificially deteriorated seeds and the time‐dependence of different spectral components was analyzed in detail. The single seed viability was evaluated through observation of germination properties after imbibition and compared with different parameters related to the luminescence kinetics. The significant correlation found between single seed delayed luminescence parameters and germination capability strongly validates the connection of this phenomenon with the functional state of the system and suggests the development of a non‐invasive technique for seed quality determination. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献
77.
Angelica Del Giudice Domenica R. Massardo Filomena Manna Natalia Koltovaya Hans Hartings Luigi Del Giudice Klaus Wolf 《Current microbiology》1997,34(6):382-384
In previous papers (Del Giudice et al. Curr Genet
8:493–497, 1984; Massardo et al. Curr Genet 17:455–457, 1985) we have shown
that strains of Saccharomyces cerevisiae that are devoid of
mitochondrial DNA (rho
o) are resistant to the alkaloid
lycorine isolated from Amaryllis plants, whereas strains containing
mitochondrial DNA (rho
−
, mit
−
,
or rho
+
) are sensitive to this drug. In addition, we were
able to show that the so-called hypersuppressive petites, whose mitochondrial
genomes consist of short regions of DNA containing an ori sequence,
show intermediate resistance. In this paper, we demonstrate that the degree
of suppressiveness of a rho
− mutant correlates with the
degree of resistance to lycorine.
Received: 20 September 1996 / Accepted: 10 January 1997 相似文献
78.
Ferreira Flavia V. Musumeci Matías A. 《World journal of microbiology & biotechnology》2021,37(5):1-24
World Journal of Microbiology and Biotechnology - Industrialisation, directly or indirectly, exposes humans to various xenobiotics. The increased magnitude of chemical pesticides and toxic heavy... 相似文献
79.
Biancamaria Farina Annarita Del Gatto Daniela Comegna Sonia Di Gaetano Domenica Capasso Carla Isernia Michele Saviano Roberto Fattorusso Laura Zaccaro Luigi Russo 《Journal of peptide science》2019,25(5)
Integrins are heterodimeric cell‐surface proteins that play important roles during developmental and pathological processes. Diverse human pathologies involve integrin adhesion including thrombotic diseases, inflammation, tumour progression, fibrosis, and infectious diseases. Although in the past decade, novel integrin‐inhibitor drugs have been developed for integrin‐based medical applications, the structural determinants modulating integrin‐ligands recognition mechanisms are still poorly understood, reducing the number of integrin subtype exclusive antagonists. In this scenario, we have very recently showed, by means of chemical and biological assays, that a chimeric peptide (named RGDechi), containing a cyclic RGD motif linked to an echistatin C‐terminal fragment, is able to interact with the components of integrin family with variable affinities, the highest for αvβ3. Here, in order to understand the mechanistic details driving the molecular recognition mechanism of αvβ3 by RGDechi, we have performed a detailed structural and dynamics characterization of the free peptide by natural abundance nuclear magnetic resonance (NMR) spectroscopy. Our data indicate that RGDechi presents in solution an heterogeneous conformational ensemble characterized by a more constrained and rigid pentacyclic ring and a largely unstructured acyclic region. Moreover, we propose that the molecular recognition of αvβ3 integrin by RGDechi occurs by a combination of conformational selection and induced fit mechanisms. Finally, our study indicates that a detailed NMR characterization, by means of natural abundance 15N and 13C, of a mostly unstructured bioactive peptide may provide the molecular basis to get essential structural insights into the binding mechanism to the biological partner. 相似文献
80.
This work deals with the Dakin-West synthesis, starting from the nucleoamino acid 1-thyminyl acetic acid, as well the NMR, ESI MS, and X-ray characterization of a heteroaromatic compound denominated by us T(2)CO, comprising two thymine moieties anchored to a 2-propanonic unit, the spectroscopic properties of which were studied by UV as a function of temperature and ionic strength. Preliminary binding-studies with molecules of biomedical interest such as nucleic acids and proteins, performed on samples containing T(2)CO, suggested that this molecule is able to interact very weakly with double-stranded RNA, whereas it does not seem to bind other nucleic acids or proteins. Moreover, by studies with fresh human serum we found that T(2)CO is resistant to enzymatic degradation till 24?h, whereas UV metal binding-studies, performed using solutions of copper (II) chloride dihydrate and nickel (II) chloride hexahydrate, revealed a certain ability of T(2)CO to bind copper (II) cation. Finally, by CD spectroscopy we investigated the influence of T(2)CO on the already described supramolecular networks based on L-serine-containing nucleopeptides. More particularly, we found that T(2)CO is able to increase the level of structuration of the non-covalent supramolecular assembly of the chiral nucleopeptides, which is a feature of remarkable interest for the development of innovative drug delivery tools. 相似文献