全文获取类型
收费全文 | 369篇 |
免费 | 12篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 9篇 |
2020年 | 4篇 |
2019年 | 5篇 |
2018年 | 5篇 |
2017年 | 6篇 |
2016年 | 11篇 |
2015年 | 15篇 |
2014年 | 14篇 |
2013年 | 17篇 |
2012年 | 33篇 |
2011年 | 21篇 |
2010年 | 17篇 |
2009年 | 6篇 |
2008年 | 29篇 |
2007年 | 30篇 |
2006年 | 18篇 |
2005年 | 21篇 |
2004年 | 23篇 |
2003年 | 19篇 |
2002年 | 13篇 |
2001年 | 5篇 |
1999年 | 2篇 |
1998年 | 4篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 4篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 3篇 |
1981年 | 5篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1973年 | 1篇 |
1967年 | 1篇 |
1965年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有381条查询结果,搜索用时 265 毫秒
51.
Srboljub M Mijailovich Milos Kojic Miroslav Zivkovic Ben Fabry Jeffrey J Fredberg 《Journal of applied physiology》2002,93(4):1429-1436
Magnetic twisting cytometry probes mechanical properties of an adherent cell by applying a torque to a magnetic bead that is tightly bound to the cell surface. Here we have used a three-dimensional finite element model of cell deformation to compute the relationships between the applied torque and resulting bead rotation and lateral bead translation. From the analysis, we computed two coefficients that allow the cell elastic modulus to be estimated from measurements of either bead rotation or lateral bead translation, respectively, if the degree of bead embedding and the cell height are known. Although computed strains in proximity of the bead can be large, the relationships between applied torque and bead rotation or translation remain virtually linear up to bead rotations of 15 degrees, above which geometrical nonlinearities become significant. This appreciable linear range stands in contrast to the intrinsically nonlinear force-displacement relationship that is observed when cells are indented during atomic force microscopy. Finally, these computations support the idea that adhesive forces are sufficient to keep the bead firmly attached to the cell surface throughout the range of working torques. 相似文献
52.
53.
Mary Holland Gerald Rhodes Mark DalleAve Donald Wiesler Milos Novotny 《Life sciences》1983,32(7):787-794
Qualitative and quantitative differences in the urinary excretion of volatile and acidic metabolites in germfree and conventional rats were examined by capillary gas chromatography and gas chromatography/mass spectrometry. A number of carbonyl compounds, including several short-chain aliphatic ketones and acetophenone, were higher in the conventional urines, while many heterocyclic compounds (furan derivatives, benzothiazole and others) were lower. Both qualitative and quantitative differences were observed in the urinary excretion of acidic metabolites. Three meta-hydroxy phenolic acids appeared only in the conventional rat urines, while levels of many other aromatic and aliphatic acids were also higher. 相似文献
54.
Milos Zelinka 《Hydrobiologia》1973,42(1):13-19
The first part (1969) dealt with the abundance and biomass of the may-flies (Ephemeroptera). On the basis of the measurement of length of all may-flies and of the determination of the respective weight (according to the curve expressing the relation length : weight) the production for each month was calculated. The annual average for Baetis rhodani amounted to 5,317 g/m2, for Rhithrogena semicolorata 12,478 and for the species belonging to the genus Ecdyonurus 8,47 g/m2. The relation between the annual production and average annual biomass was nearly the same in all three species = 1 : 8,37. These three taxa formed 76% of the biomass. After addition of the remaining taxa of mayflies the production of Ephemeroptera in the two brooks under investigation totalled 27,152 g/m2 = 271,52 kg/ha per year. 相似文献
55.
The Impact of Genetic Removal of GFAP and/or Vimentin on Glutamine Levels and Transport of Glucose and Ascorbate in Astrocytes 总被引:12,自引:0,他引:12
Pekny Milos Eliasson Camilla Siushansian Ramin Ding Mei Dixon S. Jeffrey Pekna Marcela Wilson John X. Hamberger Anders 《Neurochemical research》1999,24(11):1357-1362
The importance of the intermediate filament (IF) proteins glial fibrillary acidic protein (GFAP) and vimentin for astrocyte function was studied by investigating astrocytes prepared from GFAP-/-and/or vimentin-/- mice. The rate of glucose uptake through facilitative hexose transporters was not affected by depletion of GFAP or vimentin. Similarly, the absence of these IF proteins did not affect ascorbate uptake, under control or cyclic AMP-stimulated conditions, or ascorbate efflux through volume-sensitive organic anion channels. However, compared with wild-type astrocytes, glutamine concentrations were increased up to 200% in GFAP-/- astrocytes and up to 150% in GFAP+/-astrocytes and this increase was not dependent on the presence of vimentin. GFAP-/- astrocytes in culture still contain IFs (made of vimentin and nestin), whereas GFAP-/-vim-/- cultured astrocytes lack IFs. Thus, glutamine levels appear to correlate inversely with GFAP, rather than depend on the presence of IFs per se. Furthermore, the effect of GFAP is dose-dependent since the glutamine concentration in GFAP+/- astrocytes falls between those in wild-type and GFAP-/-astrocytes. 相似文献
56.
Pavlina Lyskova Milos Kubanek Vit Hubka Eva Sticova Ludek Voska Dana Kautznerova Miroslav Kolarik Petr Hamal Martina Vasakova 《Mycopathologia》2017,182(3-4):297-303
We report a case of phaeohyphomycosis caused by Alternaria infectoria in a 61-year-old heart transplant recipient with multiple skin lesions and pulmonary infiltrates. The infection spread via the haematogenous route from the primary cutaneous lesions into the lungs. The diagnosis was based on the histopathological examination, direct microscopy, skin lesion cultures and detection of Alternaria DNA in the bronchoalveolar lavage fluid using molecular methods. The treatment consisted of a combination of surgical excision and systemic antifungal therapy. Voriconazole was the first agent used but had a weak effect. Posaconazole was subsequently used to achieve a successful response. The isolate was identified as A. infectoria by sequencing of the rDNA ITS region and the partial β-tubulin gene. 相似文献
57.
Lasioglossins LLIII affect the morphogenesis of Candida albicans and reduces the duration of experimental vaginal candidiasis in mice
下载免费PDF全文
![点击此处可从《Microbiology and immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Alena Vrablikova Lydie Czernekova Romana Cahlikova Zbynek Novy Milos Petrik Saima Imran Zdenek Novak Michal Krupka Vaclav Cerovsky Jaroslav Turanek Milan Raska 《Microbiology and immunology》2017,61(11):474-481
58.
Kvasnica M Budesinsky M Swaczynova J Pouzar V Kohout L 《Bioorganic & medicinal chemistry》2008,16(7):3704-3713
Twelve steroidal platinum(II) complexes were synthesized by reaction of potassium tetrachloroplatinate with steroidal esters of L-methionine and L-histidine. The steroidal esters coordinated as bidentate ligands via S and N donor atoms of L-methionine and via two N donor atoms of L-histidine. Cholesterol, cholestanol, diosgenine, pregnenolone, dehydroepiandrosterone, testosterone, estrone, and estradiol were used as the steroidal compounds. The esters and complexes prepared were characterized by infrared, mass, and (1)H NMR spectroscopy and elemental analysis. Platinum complexes were tested for in vitro cytotoxicity against several cancer cell lines: T-lymphoblastic leukemia CEM, breast carcinoma MCF-7, lung carcinoma A-549, multiple myeloma RPMI 8226, and one normal cell line human fibroblast BJ. 相似文献
59.
Seventeen additional minor ecdysteroid compounds were isolated and identified from the roots of Leuzea carthamoides (Wild.) DC. Eight of them are new phytoecdysteroids: carthamoleusterone (13) is a new side-chain cyclo-ether with five-membered ring; 14-epi-ponasterone A 22-glucoside (12) is a rare and unusual natural 14 beta-OH epimer; 15-hydroxyponasterone A (11) is also new and rare with its C-15 substituted position, as well as 22-deoxy-28-hydroxymakisterone C (18) possessing secondary hydroxyl in position C-28 and 26-hydroxymakisterone C (20) with hydroxy groups in positions 25 and 26. New are also 1 beta-hydroxymakisterone C (21) and 20,22-acetonides of inokosterone (8) and integristerone A (10). Series of already known ecdysteroids: ecdysone (1), 20-hydroxyecdysone 2- and 3-acetates (3 and 4), turkesterone (6), inokosterone (7), 24-epi-makisterone A (14), and amarasterone A (22) are reported here as new constituents of L. carthamoides. Seven earlier reported Leuzea ecdysteroids: 20-hydroxyecdysone (2), ajugasterone C (5), integristerone A (9), 24(28)-dehydromakisterone A (15), 24(28)-dehydroamarasterone B (16), (24Z)-29-hydroxy-24(28)-dehydromakisterone C (17) and makisterone C (19) are also included because they are now better characterized. 相似文献
60.
Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical 总被引:1,自引:0,他引:1
Spasojević I Mojović M Blagojević D Spasić SD Jones DR Nikolić-Kokić A Spasić MB 《Carbohydrate research》2009,344(1):80-2259
The hydroxyl radical (OH) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P); fructose-6-phosphate (F6P); and fructose-1,6-bis(phosphate) (F16BP)) on OH radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of OH radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate OH, so that the effect of iron sequestration could be distinguished from direct OH radical scavenging. In the latter system, the rank order of OH scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body’s reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy. 相似文献