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991.
AbstractAim: Cardiac autonomic neuropathy (CAN) is a common and important chronic complication in diabetic patients. Heart failure resulting from cardiomyopathy is also a lethal complication in diabetic patients. However, data showing the exact association between CAN and heart failure in diabetic patients are relatively scarce. Therefore, our study aimed to determine the association between the parameters assessing CAN and heart function in diabetic patients.Method: The medical records of type 2 diabetic patients who underwent an autonomic function test with heart rate variability (HRV) and echocardiography were reviewed from January 2018 to December 2018. A total of 100 type 2 diabetic patients were included, and the association between the parameters assessing CAN and heart function was analysed.Results: Among the 100 analysed patients, 65 were diagnosed with CAN and 26 showed diastolic dysfunction. Moreover, 19 (73.1%) diabetic patients with diastolic dysfunction were complicated with CAN. The occurrence of diastolic dysfunction was higher in diabetic patients with CAN than in diabetic patients without CAN (29.2% vs 20.0%, p?<?0.05), and the occurrence of CAN was higher in diabetic patients with diastolic dysfunction than in patients without diastolic dysfunction (73.1% vs 62.2%, p?<?0.05). However, there were no significant associations between HRV parameters and heart function.Conclusion: We demonstrated that diastolic dysfunction is more common in diabetic patients complicated with CAN than in diabetic patients without CAN, although several diabetic patients without diastolic dysfunction are also diagnosed with CAN. Moreover, further studies about the long-term serial monitoring of heart function according to the progression of CAN are required to confirm the exact association between CAN and heart function. 相似文献
992.
993.
Excessive iodine intake is known to induce hypothyroidism in people who have underlying thyroid disorders. However, few studies
have been performed on subjects with normal thyroid function without a history of autoimmune thyroid disease. We hypothesized
that high iodine intake may cause a subtle change in thyroid function even in subjects with normal thyroid function. We analyzed
337 subjects (64 men and 273 women; mean age, 49 years) who showed normal levels of thyroid peroxidase antibodies (TPO-Ab)
and thyroglobulin antibodies (Tg-Ab) by measuring the urinary iodine excretion, free T4 (FT4), and thyroid-stimulating hormone
(TSH). The results showed urinary iodine excretion had negative correlation with FT4 (γ = −0.11, p = 0.043) and showed a positive trend with TSH (γ = 0.10, p = 0.068). We found that 61.7% of subjects had circulating TPO-Ab within normal reference range. In all subjects, TPO-Ab levels
were negatively correlated with FT4 (γ = −0.17, p = 0.002) and positively with TSH (γ = 0.13, p = 0.021). In conclusion, high iodine intake can negatively affect thyroid hormone levels in subjects with normal thyroid
function. Population-based study will be helpful for further clarification. 相似文献
994.
Jong Min Lee Ha Young Chung Kyung Il Kim Ki Hyun Yoo Jeon Hwang-Bo In Sik Chung Jong-Hwa Park 《Biotechnology letters》2011,33(1):41-46
We established a bicistronic expression system using an encephalomyocarditis virus (EMCV)-derived internal ribosomal entry
site (IRES) element to generate stably transformed Drosophila
melanogaster Schneider 2 (S2) cells expressing human rotavirus Wa capsid proteins, VP2 and VP6, for the synthesis of VP2/6 double-layered
virus-like particle (DVLP). The EMCV-derived IRES permitted bicistronic translation of recombinant VP6. Recombinant VP2 and
VP6 were detected in extracellular fractions of stably transformed S2 cells. A wheel-like DVLP (diam ~ 50–55 nm) with short
spikes was produced from the extracellular fraction of stably transformed S2 cells. A bicistronic expression system using
an EMCV-derived IRES element can thus be used to express two proteins of interest in stably transformed S2 cells. The bi-or
tri-cistronic expression of recombinant VP2/6/7 using stably transformed S2 cells can also be used to produce rotavirus VLPs. 相似文献
995.
Efficient production of polylactic acid and its copolymers by metabolically engineered Escherichia coli 总被引:1,自引:0,他引:1
Polylactic acid (PLA) is one of the promising biodegradable polymers, which has been produced in a rather complicated two-step process by first producing lactic acid by fermentation followed by ring opening polymerization of lactide, a cyclic dimer of lactic acid. Recently, we reported the production of PLA and its copolymers by direct fermentation of metabolically engineered Escherichia coli equipped with the evolved propionate CoA-transferase and polyhydroxyalkanoate (PHA) synthase using glucose as a carbon source. When employing these initially constructed E. coli strains, however, it was necessary to use an inducer for the expression of the engineered genes and to feed succinate for proper cell growth. Here we report further metabolic engineering of E. coli strain to overcome these problems for more efficient production of PLA and its copolymers. This allowed efficient production of PLA and its copolymers without adding inducer and succinate. The finally constructed recombinant E. coli JLXF5 strain was able to produce P(3HB-co-39.6 mol% LA) having the molecular weight of 141,000 Da to 20 g l−1 with a polymer content of 43 wt% in a chemically defined medium by the pH-stat fed-batch culture. 相似文献
996.
Lee MH Chung CY Lee KM Seong WK Kim KS Lee SD Park MS 《Journal of biotechnology》2011,153(3-4):176-180
This study aimed to investigate precision of a newly developed gold nanoparticle-based immunoassay for measuring urinary N-telopeptides (NTx) and its correlation with ELISA measurement. NTx concentrations were determined by absorption changes using spectroscopy at 535 nm, which were measured three times with an interval of two hours for precision test, using urine samples of 60 subjects (32 males and 28 females; mean age 30.4 years, SD 21.9 years). The correlation of the immunoassay with the measurement of ELISA kit was also analyzed. Precision of the immunoassay and its correlation with ELISA were 0.924 (ICC, 95% confidence interval, 0.661-0.971) and 0.90 (p<0.001). Our findings suggested that this immunoassay of urinary NTx could be used as a means of screening for bone resorption and point-of-care testing. 相似文献
997.
In our previous study using a fluorescently labeled cohesin biomarker, we detected and identified a putative cellulosomal
mannanase belonging to the glycosyl hydrolase family 26 from Clostridium cellulovorans in xylan-containing cultures. In this study, a mannanase gene, manB from C. cellulovorans, was expressed in Escherichia coli. The optimal pH of a purified enzyme was around pH 7.0 and the optimal temperature was 40°C. The purified mannanase B (ManB)
showed high hydrolytic activity toward galactomannan. An assembly of ManB with mini-CbpA, which contains a carbohydrate-binding
module that provides proximity to insoluble substrates, increased the activity toward galactomannan [locust bean gum (LBG)
and guar gum] 1.7- and 2.0-fold over those without mini-CbpA. We tested the synergistic effects on galactomannan (LBG and
guar gum) degradation using cellulosomal mannanase ManB with cellulosomal endoglucanase E, which was predicted to have mannanase
activity in C. cellulovorans as a cellulolytic complex. When assembled with the mini-CbpA, the mixture of endoglucanase E (EngE) and ManB at a molar ratio
of 1:2 showed the highest synergistic effect (2.4-fold) on LBG. The mixture at a ratio of 1:3 showed the highest synergistic
effect (2.8-fold) on guar gum. These synergistic actions indicated that ManB assembled with mini-CbpA hydrolyzed insoluble
galactomannan, which in turn promoted soluble galactomannan degradation by EngE. 相似文献
998.
Choi JH Yoo KY Lee CH Yi SS Yoo DY Seong JK Yoon YS Hwang IK Won MH 《Cellular and molecular neurobiology》2011,31(2):285-292
N-methyl-d-aspartate receptor (NR) is involved in activity-dependent synaptic plasticity, such as associative long-term potentiation,
and in related central functions, such as learning and memory. In this study, we observed effects of treadmill exercise on
NR1 and doublecortin (DCX, a marker for neuroblast differentiation) in the subgranular zone of the dentate gyrus (DG). At
6 weeks of age, rats were put on a treadmill with or without running for 1 h/day for 5 consecutive days at 22 m/min for 5 weeks.
Exercise increased NR1 immunoreactivity and protein level in the hippocampus. To identify the correlations between NR and
neuroblasts, we intraperitoneally administered a NR antagonist, MK-801, to the exercised rats. MK-801 treatment reduced NR1
protein level in the hippocampus of the exercised rats. In addition, in the MK-801-treated group, the number of DCX cells
was significantly decreased in the subgranular zone of the DG. These results suggest that NR may be one of the important factors
that modulate neuroblast differentiation during exercise in rats. 相似文献
999.
Subramanya Srikantan Kotb Abdelmohsen Eun Kyung Lee Kumiko Tominaga Sarah S. Subaran Yuki Kuwano Ritu Kulshrestha Rohit Panchakshari Hyeon Ho Kim Xiaoling Yang Jennifer L. Martindale Bernard S. Marasa Mihee M. Kim Robert P. Wersto Fred E. Indig Dipanjan Chowdhury Myriam Gorospe 《Molecular and cellular biology》2011,31(18):3790-3801
1000.
Tominaga K Srikantan S Lee EK Subaran SS Martindale JL Abdelmohsen K Gorospe M 《Molecular and cellular biology》2011,31(20):4219-4231