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21.
N G Nasirov B I Sukhorukov L I Shabarchina A I Petrov 《Molekuliarnaia biologiia》1991,25(4):1024-1032
The change in survival of bacteriophages with DNA of different GC-contents after their incubation in media of different acidities with subsequent neutralization was studied. It was shown that the higher the GC-content, the more sensitive is the phage to the action of H(+)-ions. Evidence is presented that the acidic inactivation of virions is not connected with the helix-coil transition of the intraphage DNA due to its protonation. The extractability of DNA from phages subjected to different concentrations of H(+)-ions with subsequent neutralization of the medium to pH 8 was determined. The changes in: transfection ability, UV-spectra, the quantity of the residual proteins, and the contents of glutamic and lysine amino acid residues in these proteins were investigated. The effect of glutamic acid on the parameters of DNA melting curves was followed for different pH values. Proceeding from the data obtained, we concluded that acidification of the medium from neutral tp pH congruent to 4 leads to formation of non-covalent DNA-protein cross-links due to interaction of the GC base pairs of DNA with glutamic and aspartic amino acid residues, whereas acidification of the medium to pH less than 4 with subsequent neutralization to pH 8 results in the formation of covalent DNA-protein cross-links of Schiff base type. The influence of non-covalent DNA-protein cross-links on the properties of DNA and their regulatory role in genome functioning are discussed. 相似文献
22.
A study of the level of fibronectin in blood serum of Wistar rats at different times after gamma-irradiation (7.5 Gy) has demonstrated a considerable increase in its concentration after 3 days. This increase is a function of radiation dose absorbed (within the range from 1 to 10 Gy). The most pronounced changes are observed with serious and extremely serious forms of acute radiation sickness. The administration of cycloheximide 48 h after irradiation removes completely the increase in the level of blood serum fibronectin. 相似文献
23.
V V Svetlichkin A M Lysenko V G Ignashov N B Petrov 《Nauchnye doklady vysshe? shkoly. Biologicheskie nauki》1985,(10):106-110
Different methods of isotope introduction into plasmid and chromosomal DNA have been compared. The efficiency of label introduction into DNA was estimated by the results of DNA--DNA hybridisation and by the thermostability of hybrid duplexes. Using the enzymatic methods of label introduction levels of DNA specific radioactivity and label binding in homologous and heterologous reactions were close. DNA labeled by the method of enzymatic methylation forms more thermostable hybrid duplexes than "nick-translation" DNA. The advantages of enzymatic methods of label introduction for creating a microorganism reference DNA bank are discussed. 相似文献
24.
A study was made of the changes in the mode of interaction between T- and B-lymphocytes of mouse lymph nodes with respect to the phenomenon of inactivation of non-syngeneic haemopoietic stem cells. It was shown that irradiation of B-lymphocytes with doses of 77.4--232.2 mC/kg changes their helper activity into a suppressor activity with regard to T-cell-killers having a low electrophoretic mobility. 相似文献
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Segmental mobility dynamic peculiarities of poly(U), poly(A) and poly(C) synthetic polymers and their complexes were investigated by spin-label method. Imidazolide spin-label was introduced into 2'-oxi-groups of polymer ribose in correlation: one spin-label on 18-20 bases. Formation of complexes was observed by ESR spectra at two pH: 4.2 and 7.2. Segmental mobility of only single strand spin-labelled polymer segment and in the complex was evaluated by measuring rotational correlation time (tau) determined by dependence of distances between outer wide extrema in ESR spectra from solvent viscosity at different temperatures. It turned out that correlation time tau of single strand structures in a high degree depend on pH and temperature. For three strand structures abrupt increase of tau because of appearance of rigidity was observed. It is possible to evaluate part of triple complexes poly(U.A.A) and poly(U.U.A) existing in dynamic equilibrium depending on pH and temperature by the form of outer wide extrema. Adding of dye to complex of poly(U).poly(A) causes an increase of rigidity of the supermolecular structure. Quantitative characteristics of formed complexes were obtained by simulation of ESR spectra on computer. 相似文献
28.
The authors own results on the variety of the genomic primary structures in human influenza A viruses participating in the epidemic process, including the atypical viruses. The comparative studies revealed new trends in the HA gene antigenic drift on the late stages and the PB1 gene shift. Modifications occurring in the primary structure of the influenza A viruses native genomes during laboratory treatment (adaptation to new hosts, vaccine preparation, egg passaging) have been analyzed. Sequencing of several types of "antigenic anachronisms" revealed the direct links between some of such viruses and the anthropogenic pollution of the biosphere by vaccine strains. Modifications in the HA genes of influenza A viruses during the persistent infection have also been studied. 相似文献
29.
Dmitry V. Beletsky 《Hydrobiologia》1996,322(1-3):75-80
The results of numerical modelling of large-scale circulation in Lakes Onega and Ladoga are presented, with primary emphasis on the temporal variability of currents with time scales of days. Some typical circulation patterns have been inferred from model calculations. They reflect the existence of different dynamic regimes in the lakes, namely, forced and free circulation regimes. The forced circulation regime is the well-known wind-induced double-gyre circulation accompanied by coastal upwelling and downwelling. A case of double-gyre circulation in Lake Onega was investigated in particular detail. The second dynamic regime is a free response (or a relaxation) of the stratified lake to wind cessation, and is connected closely with the evolution of wind-induced upwelling and thermal front propagation. Diagnostic calculations demonstrate that the regime of relaxation supports the restoration of cyclonic circulation in Lake Onega. Barotropic circulation patterns in Lake Ladoga were calculated with the emphasis on prevailing winds from west to south-east. Our calculations show that the bottom relief of Lake Ladoga causes asymmetry in the double-gyre circulation patterns. In particular, approximately equal cyclonic and anticyclonic circulation cells appearing in the case of southerly wind transform to a single dominant cyclonic cell and several small anticyclonic cells in the case of westerly wind. We also found especially strong sensitivity of the sense of rotation of the largest gyre to the east-west components of the wind vector. 相似文献
30.
THELASERTOMOGRAPHICALMETHODUSINGMINIMUMOFPROJECTIONFORBIOLOGICALOBJECTSTRUCTURESTUDYYuriN.Kulchin;OlegB.Vitrik;OlegV.Kirichei... 相似文献