全文获取类型
收费全文 | 1589篇 |
免费 | 117篇 |
国内免费 | 135篇 |
专业分类
1841篇 |
出版年
2024年 | 2篇 |
2023年 | 17篇 |
2022年 | 52篇 |
2021年 | 77篇 |
2020年 | 59篇 |
2019年 | 86篇 |
2018年 | 77篇 |
2017年 | 57篇 |
2016年 | 82篇 |
2015年 | 101篇 |
2014年 | 110篇 |
2013年 | 125篇 |
2012年 | 127篇 |
2011年 | 114篇 |
2010年 | 76篇 |
2009年 | 74篇 |
2008年 | 84篇 |
2007年 | 58篇 |
2006年 | 59篇 |
2005年 | 48篇 |
2004年 | 48篇 |
2003年 | 39篇 |
2002年 | 37篇 |
2001年 | 31篇 |
2000年 | 34篇 |
1999年 | 35篇 |
1998年 | 13篇 |
1997年 | 13篇 |
1996年 | 15篇 |
1995年 | 15篇 |
1994年 | 8篇 |
1993年 | 8篇 |
1992年 | 10篇 |
1991年 | 9篇 |
1990年 | 10篇 |
1989年 | 5篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1986年 | 6篇 |
1985年 | 7篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1980年 | 1篇 |
排序方式: 共有1841条查询结果,搜索用时 15 毫秒
71.
Dayong Xia Xiaofu Zhai Honglian Wang Zhiyong Chen Chuanjing Fu Meihua Zhu 《Journal of cellular and molecular medicine》2019,23(6):4088-4096
Alpha lipoic acid (ALA) is a powerful antioxidant which has been widely used in the treatment of different system diseases, such as cardiovascular and cerebrovascular diseases. But, there are few studies that refer to protective effects and potential mechanisms on traumatic brain injury (TBI). This study was carried out to investigate the neuroprotective effect following TBI and illuminate the underlying mechanism. Weight drop‐injured model in rats was induced by weight‐drop. ALA was administrated via intraperitoneal injection after TBI. Neurologic scores were examined following several tests. Neurological score was performed to measure behavioural outcomes. Nissl staining and TUNEL were performed to evaluate the neuronal apoptosis. Western blotting was engaged to analyse the protein content of the Nuclear factor erythroid 2‐related factor 2 (Nrf2) and its downstream protein factors, including hemeoxygenase‐1 (HO‐1) and quinine oxidoreductase‐1 (NQO1). ALA treatment alleviated TBI‐induced neuron cell apoptosis and improved neurobehavioural function by up‐regulation of Nrf2 expression and its downstream protein factors after TBI. This study presents new perspective of the mechanisms responsible for the neuronal apoptosis of ALA, with possible involvement of Nrf2 pathway. 相似文献
72.
Lei Qin Yu Lei Huwei Wang Jiahui Dong Yiying Wu Dengyun Zhai Feiyu Kang Ying Tao Quan‐Hong Yang 《Liver Transplantation》2019,9(29)
Metallic potassium (K) is a desirable anode for potassium secondary batteries due to its low electrode potential in nonaqueous electrolytes and high theoretical capacity. Nevertheless, instability caused by dendritic growth, large volume changes, and parasitic side reactions hamper its practical application. Here, an anode containing metallic K is fabricated by infiltrating an aligned carbon nanotube membrane (ACM) with molten K because of its good wettability to molten K due to the strong capillary forces. The K metal is spatially distributed on the 3D ACM framework, which offers sufficient electrode/electrolyte contact for charge transfer. The robust ACM host provides a large number of K nucleation sites and physically confines the K deposited there, thus mitigating dimensional changes during cycling. The pathways for electrons and ions in the anode are associated to form a mixed conducting network, which is beneficial for the electrochemical redox. Consequently, the anode shows stable plating/stripping profiles with low polarization in symmetric cells using conventional carbonate‐based electrolytes. In addition, dendrite growth is suppressed, and the anode demonstrates excellent suitability when paired with a Prussian blue cathode in a full cell. This design strategy is expected to provide a way to address the problems with using metallic K anodes. 相似文献
73.
Inhibition of the adenosinergic pathway: the indispensable part of oncological therapy in the future
Huang Yi Gu Zili Fan Yang Zhai Guangxi Zhao Xiaogang Sun Qifeng Shi Yanbin Lin Guimei 《Purinergic signalling》2019,15(1):53-67
Purinergic Signalling - In recent years, immunotherapy has produced many unexpected breakthroughs in oncological therapy; however, it still has many deficiencies. For example, the number of... 相似文献
74.
Zhai Xiaofeng Zhao Wen Li Kemang Zhang Cheng Wang Congcong Su Shuo Zhou Jiyong Lei Jing Xing Gang Sun Haifeng Shi Zhiyu Gu Jinyan 《中国病毒学》2019,34(6):601-609
Since late 2011, outbreaks of pseudorabies virus(PRV) have occurred in southern China causing major economic losses to the pig industry. We previously reported that variant PRV forms and recombination in China could be the source of continued epidemics. Here, we analyzed samples from intensive pig farms in eastern China between 2017 and 2019, and sequenced the main glycoproteins(gB, gC, gD, and gE) to study the evolution characteristics of PRV. Based on the g C gene, we found that PRV variants belong to clade 2 and detected a founder effect during by the PRV epidemic. In addition,we detected inter-and intra-clade recombination; in particular, inter-clade recombination in the g B genes of strains FJ-ZXF and FJ-W2, which were recombinant with clade 1 strains. We also found specific amino-acid changes and positively selected sites, possibly associated with functional changes. This analysis of the emergence of PRV in China illustrates the need for continuous monitoring and the development of vaccines against specific variants of PRV. 相似文献
75.
WEICHAO Zhai DERRICK YONG JEHAN JOMAA EL-JAWHARI RICHARD CUTHBERT DENNIS MCGONAGLE MAY WIN NAING ELENA JONES 《Cytotherapy》2019,21(8):803-819
Regardless of their tissue of origin, multipotent mesenchymal stromal cells (MSCs) are commonly expanded in vitro for several population doublings to achieve a sufficient number of cells for therapy. Prolonged MSC expansion has been shown to result in phenotypical, morphological and gene expression changes in MSCs, which ultimately lead to the state of senescence. The presence of senescent cells in therapeutic MSC batches is undesirable because it reduces their viability, differentiation potential and trophic capabilities. Additionally, senescent cells acquire senescence-activated secretory phenotype, which may not only induce apoptosis in the neighboring host cells following MSC transplantation, but also trigger local inflammatory reactions. This review outlines the current and promising new methodologies for the identification of senescent cells in MSC cultures, with a particular emphasis on non-destructive and label-free methodologies. Technologies allowing identification of individual senescent cells, based on new surface markers, offer potential advantage for targeted senescent cell removal using new-generation senolytic agents, and subsequent production of therapeutic MSC batches fully devoid of senescent cells. Methods or a combination of methods that are non-destructive and label-free, for example, involving cell size and spectroscopic measurements, could be the best way forward because they do not modify the cells of interest, thus maximizing the final output of therapeutic-grade MSC cultures. The further incorporation of machine learning methods has also recently shown promise in facilitating, automating and enhancing the analysis of these measured data. 相似文献
76.
Yueming Zhang Wenrui Zhang Lina Tao Jinghui Zhai Huan Gao Yanqing Song Xiaoyu Qu 《Journal of biochemical and molecular toxicology》2019,33(9)
Isoniazid (INH) is one of the most commonly used antituberculosis drugs, but its clinical applications have been limited by severe hepatic toxicity. Quercetin (Que), a natural flavonoid, has been proved to have many medicinal properties. This study aimed to clarify the possible protective effects of Que against INH‐induced hepatotoxicity using HepG2 cells. Our results indicated that Que significantly increased cell viability, superoxide dismutase, and GSH levels, while decreased alanine aminotransferase/aspartate aminotransferase levels. Besides, Que significantly abrogated INH‐induced cell apoptosis by upregulating the expression levels of Bcl‐2 and decreasing the levels of Bax, cleaved caspase‐3, and cleaved caspase‐9. Furthermore, Que obviously reversed the inhibition of INH on Sirtuin 1 (SIRT1) expression and extracellular signal‐regulated kinase (ERK) phosphorylation. Next, the SIRT1 inhibitor EX527 blocked the enhancement of Que upon ERK phosphorylation. Notably, EX527 partially abolished the beneficial effects of Que. In brief, our results provided the first evidence that Que protected against INH‐induced HepG2 cells by regulating the SIRT1/ERK pathway. 相似文献
77.
Cai Hailin Xie Pengfei Zeng Weiai Zhai Zhengguang Zhou Wen Tang Zhong 《Molecular breeding : new strategies in plant improvement》2019,39(3):1-10
Molecular Breeding - Apple mildew, caused by the fungus Podosphaera leucotricha, is an ongoing problem for apple growers in most world production regions. To manage apple foliar mildew with less... 相似文献
78.
79.
异基因造血干细胞移植(allo-HSCT)是治愈多种非恶性病的有效方法。脐带血干细胞(UCB)具有免疫原性低、人类白细胞抗原不合耐受性好、移植物抗宿主反应发生率低以及获取相对快捷等特点,可作为非恶性血液疾病患者allo-HSCT的来源。本文简要综述脐血干细胞移植在原发性免疫缺陷病、遗传性骨髓衰竭、遗传代谢病以及自身免疫性疾病等非恶性血液疾病的治疗效果。 相似文献
80.