全文获取类型
收费全文 | 329篇 |
免费 | 24篇 |
出版年
2023年 | 2篇 |
2022年 | 9篇 |
2021年 | 8篇 |
2020年 | 1篇 |
2019年 | 9篇 |
2018年 | 11篇 |
2017年 | 8篇 |
2016年 | 12篇 |
2015年 | 18篇 |
2014年 | 18篇 |
2013年 | 27篇 |
2012年 | 20篇 |
2011年 | 31篇 |
2010年 | 14篇 |
2009年 | 21篇 |
2008年 | 27篇 |
2007年 | 30篇 |
2006年 | 18篇 |
2005年 | 8篇 |
2004年 | 16篇 |
2003年 | 13篇 |
2002年 | 10篇 |
2001年 | 6篇 |
2000年 | 1篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1993年 | 1篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
排序方式: 共有353条查询结果,搜索用时 593 毫秒
321.
322.
Catherine Argyropoulou Dimitra Daferera Petros A. Tarantilis Costas Fasseas Moschos Polissiou 《Biochemical Systematics and Ecology》2007,35(12):831-837
The chemical composition of the essential oil extracted from fresh leaves of Lippia citriodora (Verbenaceae) was analyzed by GC-FID and GC–MS in May, when growth rates are maximal, and in September, in full bloom. In both samples the main constituents were geranial, neral and limonene constituting 66.3% of the total essential oil yield in May and increasing to 69% in September. Their individual percentage values, however, changed considerably for geranial and neral decreasing from 38.7 to 26.8% and from 24.5 to 21.8%, respectively, and for limonene increasing from 5.8 to 17.7%. All other components remained more or less unchanged both qualitatively and quantitatively. FT-IR spectrometry was also applied for the qualitative determination of the main components. 相似文献
323.
324.
Structural basis for signal-sequence recognition by the translocase motor SecA as determined by NMR 总被引:4,自引:0,他引:4
Gelis I Bonvin AM Keramisanou D Koukaki M Gouridis G Karamanou S Economou A Kalodimos CG 《Cell》2007,131(4):756-769
Recognition of signal sequences by cognate receptors controls the entry of virtually all proteins to export pathways. Despite its importance, this process remains poorly understood. Here, we present the solution structure of a signal peptide bound to SecA, the 204 kDa ATPase motor of the Sec translocase. Upon encounter, the signal peptide forms an alpha-helix that inserts into a flexible and elongated groove in SecA. The mode of binding is bimodal, with both hydrophobic and electrostatic interactions mediating recognition. The same groove is used by SecA to recognize a diverse set of signal sequences. Impairment of the signal-peptide binding to SecA results in significant translocation defects. The C-terminal tail of SecA occludes the groove and inhibits signal-peptide binding, but autoinhibition is relieved by the SecB chaperone. Finally, it is shown that SecA interconverts between two conformations in solution, suggesting a simple mechanism for polypeptide translocation. 相似文献
325.
Doulias PT Kotoglou P Tenopoulou M Keramisanou D Tzavaras T Brunk U Galaris D Angelidis C 《Free radical biology & medicine》2007,42(4):567-577
Heat shock protein-70 (Hsp70) is the main heat-inducible member of the 70-kDa family of chaperones that assist cells in maintaining proteins functional under stressful conditions. In the present investigation, the role of Hsp70 in the molecular mechanism of hydrogen peroxide-induced DNA damage to HeLa cells in culture was examined. Stably transfected HeLa cell lines, overexpressing or lacking Hsp70, were created by utilizing constitutive expression of plasmids containing the functional hsp70 gene or hsp70-siRNA, respectively. Compared to control cells, the Hsp70-overexpressing ones were significantly resistant to hydrogen peroxide-induced DNA damage, while Hsp70-depleted cells showed an enhanced sensitivity. In addition, the "intracellular calcein-chelatable iron pool" was determined in the presence or absence of Hsp70 and found to be related to the sensitivity of nuclear DNA to H(2)O(2). It seems likely that the main action of Hsp70, at least in this system, is exerted at the lysosomal level, by protecting the membranes of these organelles against oxidative stress-induced destabilization. Apart from shedding additional light on the mechanistic details behind the action of Hsp70 during oxidative stress, our results indicate that modulation of cellular Hsp70 may represent a way to make cancer cells more sensitive to normal host defense mechanisms or chemotherapeutic drug treatment. 相似文献
326.
Gabriela Petrof Arti Nanda Jake Howden Takuya Takeichi James?R. McMillan Sophia Aristodemou Linda Ozoemena Lu Liu Andrew?P. South Celine Pourreyron Dimitra Dafou Laura?E. Proudfoot Hejab Al-Ajmi Masashi Akiyama W.H.?Irwin McLean Michael?A. Simpson Maddy Parsons John?A. McGrath 《American journal of human genetics》2014,95(3):308-314
327.
Hadjipavlou-Litina D Magoulas GE Bariamis SE Drainas D Avgoustakis K Papaioannou D 《Bioorganic & medicinal chemistry》2010,18(23):8204-8217
A series of symmetric and asymmetric spermine (SPM) conjugates with all-trans-retinoic acid (ATRA), acitretin (ACI), (E)-3-(trioxsalen-4′-yl)acrylic acid (TRAA) and l-DOPA, amides of ACI, l-DOPA and TRAA with 1-aminobutane, benzylamine, dopamine and 1,12-diaminobutane as well as hybrid conjugates of O,O′-dimethylcaffeic acid (DMCA) with TRAA or N-fumaroyl-indole-3-carboxanilide (FICA) and 2-(2-aminoethoxy)ethanol were synthesized and their antioxidant properties were studied. The reducing activity (RA)% of the compounds were evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging assay and found to be in the range 0–92(20 min)%/96(60 min)% at 100 μM, the most powerful being the conjugates l-DOPA-SPM-l-DOPA (8, RA = 89%/96%) and l-DOPA-dopamine (13, RA = 92%/92%). Conjugate DMCA-NH(CH2CH2O)2-FICA (14) was the most powerful LOX inhibitor with IC50 33.5 μM, followed by the conjugates ACI-NHCH2Ph (10, IC50 40.5 μM), ACI-SPM-TRAA (7, IC50 41.5 μM), DMCA-NH(CH2CH2O)2-TRAA (15, IC50 65 μM), 13 (IC50 81.5 μM) and ACI-dopamine (11, IC50 87 μM). The most potent inhibitors of lipid peroxidation at 100 μM were the conjugates 15 (98%) and ACI-SPM-ACI (4, 97%) whereas all other compounds showed activities comparable or lower than trolox. The most interesting compounds, namely ATRA-SPM-ATRA (3), 4, 10, 11 and 15, as well as unconjugated compounds such as ATRA and dopamine, were studied for their anti-inflammatory activity in vivo on rat paw oedema induced by Carrageenan and found to exhibit, for doses of 0.01 mmol/mL of conjugates per Kg of rat body weight, weaker anti-inflammatory activities (3.6–40%) than indomethacin (47%) with conjugate 3 being the most potent (40%) in this series of compounds. The cytocompatibility of selected compounds was evaluated by the viability of RAMEC cells in the presence of different concentrations (0.5–50 μM) of the compounds. Conjugates 3 (IC50 2.6 μM) and 4 (IC50 4.7 μM) were more cytotoxic than the corresponding unconjugated retinoids ATRA (IC50 18.3 μM) and ACI (IC50 14.6 μM), whereas conjugate 15 (IC50 12.9 μM) was less cytotoxic than either DCSP (IC50 11.3 μM) or the tert-butyl ester of TRAA (IC50 2.9 μM). 相似文献
328.
329.
Hanski S Houbenov N Ruokolainen J Chondronicola D Iatrou H Hadjichristidis N Ikkala O 《Biomacromolecules》2006,7(12):3379-3384
Novel hierarchical nanostructures based on ionically self-assembled complexes of diblock copolypeptides and surfactants are presented. Rod-coil diblock copolypeptide poly(gamma-benzyl-L-glutamate)-block-poly(L-lysine), PBLG-b-PLL (Mn = 25,000 and 8000 for PBLG and PLL, respectively, polydispersity index 1.08), was complexed with anionic surfactants dodecanesulfonic acid (DSA) or dodecyl benzenesulfonic acid (DBSA), denoted as PBLG-b-PLL(DSA)1.0 and PBLG-b-PLL(DBSA)1.0, respectively. The complexation leading to supramolecular rod-comb architectures was studied by transmission electron microscopy (TEM), small-angle X-ray scattering (SAXS), Fourier transform infrared spectroscopy (FTIR), and polarized optical microscopy (POM). PBLG-b-PLL, PBLG-b-PLL(DBSA)1.0, and PBLG-b-PLL(DSA)1.0 self-assemble with alternating PBLG lamellae and PLL-containing lamellae with a periodicity of 27-33 nm. Within the PBLG lamellae, the rod-like PBLG helices pack with a periodicity of ca. 1.3 nm. The internal structure of the PLL-containing lamellae depends on the complexation. For pure PBLG-b-PLL, the PLL chains adopt a random coil conformation and the PLL domains are disordered. For PBLG-b-PLL(DSA)1.0, lamellar self-assembly of periodicity of 3.7 nm within the PLL(DSA)1.0 domains is observed due to crystalline packing of the linear n-dodecyl tails. For PBLG-b-PLL(DBSA)1.0 with branched dodecyl tails, a distinct SAXS reflection is observed, suggesting self-assembly within the PLL(DBSA)1.0 domains with a periodicity of 2.9 nm. However, due to the absence of higher order reflections, the internal structure cannot be conclusively assigned. The efficient plasticization which leads to fluid-like liquid crystallinity in PBLG-b-PLL(DBSA)1.0 and an alpha-helical conformation according to FTIR allows us to suggest that the PLL(DBSA)1.0 domains have a hexagonal internal structure. The interplay of self-assembly at different length scales combined with rod-like liquid crystallinity can open new routes to design functional materials. 相似文献
330.
Fine with heat,problems with water: microclimate alters water loss in a thermally adapted insular lizard
下载免费PDF全文
![点击此处可从《Oikos》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Anat Belasen Kinsey Brock Binbin Li Dimitra Chremou Efstratios Valakos Panayiotis Pafilis Barry Sinervo Johannes Foufopoulos 《Oikos》2017,126(3):447-457
Global change, including habitat isolation and climate change, has both short‐ and long‐term impacts on wildlife populations. For example, genetic drift and inbreeding result in genetic impoverishment in small, isolated populations, while species undergo range shifts or adaptive phenotypic change in response to shifts in environmental temperatures. In this study, we utilize a model system in which Holocene landscape changes have occurred to examine long‐term effects of population isolation. To examine how isolation may constrain responses to climate change, we characterized ecophysiology across land‐bridge island populations of Erhard's wall lizard Podarcis erhardii. We hypothesized that 1) small, isolated populations that are likely genetically depauperate would exhibit lower phenotypic variability; and 2) populations would be adapted to local microhabitat conditions. We compared a population at a low elevation site on the large island of Naxos with two small populations on nearby islets to determine the effects of population fragmentation. We further compared the low elevation Naxos population with two high elevation sites characterized by disparate microclimates to examine the effects of microclimate. To assess the thermal biology and ecophysiological limits of the study species we measured operative body temperatures (Te), field body temperatures (Tb), preferred temperatures (Tp), thermal tolerances (CTmax and CTmin), and evaporative water loss (EWL). Our results indicate that small, isolated populations did not exhibit thermal biology or evaporative water loss, while EWL and thermoregulatory effort varied according to microhabitat characteristics. This study integrates fine‐scale measurements with environmental data to provide a holistic view of the relationships between ecophysiology, fragmentation, and microclimate. Our methods can be applied to other ectotherms to gain a better understanding of potential impacts of global change on natural populations. 相似文献