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Neomeris (Lamouroux, 1816) is an extant taxon, the origin of which can be tracked back into Early Cretaceous times. The introduction of a new mid-Cretaceous species from Brazil, i.e., Neomeris srivastavai n. sp., offers the opportunity to review the subdivision of the genus into three subgenera, to complete the catalogue of the fossil calcareous algae of Brazil, and to point out the huge stratigraphic gap and lack of documentation between the first occurrence of the dasycladacean model of reproduction, i.e., choristospory, and the oldest record so far known of an undescribed fossil Neomeris (from Portugal). 相似文献
93.
Stirred tank bioreactor culture combined with serum‐/xenogeneic‐free culture medium enables an efficient expansion of umbilical cord‐derived mesenchymal stem/stromal cells
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Amanda Mizukami Ana Fernandes‐Platzgummer Joana G. Carmelo Kamilla Swiech Dimas T. Covas Joaquim M. S. Cabral Cláudia L. da Silva 《Biotechnology journal》2016,11(8):1048-1059
Mesenchymal stem/stromal cells (MSC) are being widely explored as promising candidates for cell‐based therapies. Among the different human MSC origins exploited, umbilical cord represents an attractive and readily available source of MSC that involves a non‐invasive collection procedure. In order to achieve relevant cell numbers of human MSC for clinical applications, it is crucial to develop scalable culture systems that allow bioprocess control and monitoring, combined with the use of serum/xenogeneic (xeno)‐free culture media. In the present study, we firstly established a spinner flask culture system combining gelatin‐based Cultispher®S microcarriers and xeno‐free culture medium for the expansion of umbilical cord matrix (UCM)‐derived MSC. This system enabled the production of 2.4 (±1.1) x105 cells/mL (n = 4) after 5 days of culture, corresponding to a 5.3 (±1.6)‐fold increase in cell number. The established protocol was then implemented in a stirred‐tank bioreactor (800 mL working volume) (n = 3) yielding 115 million cells after 4 days. Upon expansion under stirred conditions, cells retained their differentiation ability and immunomodulatory potential. The development of a scalable microcarrier‐based stirred culture system, using xeno‐free culture medium that suits the intrinsic features of UCM‐derived MSC represents an important step towards a GMP compliant large‐scale production platform for these promising cell therapy candidates. 相似文献
94.
Molecular dynamics simulations of matrix metalloproteinase 2: role of the structural metal ions 总被引:1,自引:0,他引:1
Herein we investigate the role played by the so-called "structural metal ions" in the catalytic domain of the matrix metalloproteinase 2 enzyme (MMP-2 or gelatinase A). We performed seven molecular dynamics simulations that differ in the number and position of the noncatalytic zinc and calcium ions bound to the MMP-2 catalytic domain. An additional simulation including the three fibronectin-type modules inserted into the catalytic domain was also carried out. The analysis of the trajectories confirms that the binding/removal of the structural ions does not perturb the secondary structure elements but influences the position of several solvent-exposed loop regions that are placed near the active site cleft. The position of these loops modulates the accessibility of important anchorage points for substrate binding that have been identified in the active site groove. On the basis of semiempirical quantum chemical calculations, we estimated the relative free energies of the MMP-2 models, obtaining thus that the binding of two zinc and two calcium ions to the MMP-2 catalytic domain is energetically favored. In this MMP-2 model, which shows the most compact structure, all of the substrate binding sites are readily accessible. Globally, our results help to rationalize at the atomic level the calcium and zinc dependence of the hydrolytic activity catalyzed by the MMPs. 相似文献
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Andrade SF da Silva Filho AA de O Resende D Silva ML Cunha WR Nanayakkara NP Bastos JK 《Zeitschrift für Naturforschung. C, Journal of biosciences》2008,63(7-8):497-502
Austroplenckia populnea (Celastraceae), known as "marmelinho do campo", is used in Brazilian folk medicine as antimicrobial, anti-inflammatory, and antitumoural agent. The aim of the present work was to evaluate the antimicrobial, antileishmanial and antimalarial activities of the crude hydroalcoholic extract of A. populnea (CHE) and some of its isolated compounds. The phytochemical study of the CHE was carried out affording the isolation of methyl populnoate (1), populnoic acid (2), and stigmast-5-en-3-O-beta-(D-glucopyranoside) (3). This is the first time that the presence of compound 3 in A. populnea is reported. The results showed that the CHE presents antifungal and antibacterial activities, especially against Candida glabrata and Candida albicans, for which the CHE showed IC50 values of 0.7 microg mL(-1) and 5.5 microg mL(-1), respectively, while amphotericin B showed an IC50 value of 0.1 microg mL(-1) against both microorganisms. Compounds 1-3 were inactive against all tested microorganisms. In the antileishmanial activity test against Leishmania donovani, the CHE showed an IC50 value of 52 microg mL(-1), while compounds 2 and 3 displayed an IC50 value of 18 microg mL(-1) In the antimalarial assay against Plasmodium falciparum (D6 and W2 clones), it was observed that all evaluated samples were inactive. In order to compare the effect on the parasites with the toxicity to mammalian cells, the cytotoxicity activity of the isolated compounds was evaluated against Vero cells, showing that all evaluated samples exhibited no cytotoxicity at the maximum dose tested. 相似文献
97.
Interest in the characterization of the salivary proteome has increased in the last few years. This review discusses the different techniques and methodologies applied to the separation and identification of salivary proteins. Nowadays, proteomic techniques are the state of the art for the analysis of biologic materials and saliva is no exception. 2D electrophoresis and tryptic digest analysis by mass spectrometry are the typical methodology, but new approaches using 2D liquid chromatography/mass spectrometry methods have already been introduced for saliva analysis. Due to their important physiologic role in the oral cavity, low-molecular-weight proteins and peptides are also included in this article and the methodologies discussed. 相似文献
98.
Claudinéia?R.?Pelacani Raimundo?S.?BarrosEmail author Dimas?M.?Ribeiro Renita?B.?C.?Frigeri 《Acta Physiologiae Plantarum》2005,27(3):387-394
An acidic condition (low pH) of the germination media promoted dormancy breakage of scarified seeds of Townsville stylo (Stylosanthes humilis H.B.K.), an annual tropical forage legume, whether produced by either an unbuffered (HCl-KOH) or a buffered (phthalate, McIlvaine)
medium. Except for aminooxyacetic acid, all ethylene biosynthesis inhibitors tested and supplied with the low pH solutions
decreased germination to variable extents. Low pH-stimulated dormant seeds produced ethylene 4-fold as much than untreated
seeds. Production of ethylene by seeds treated with high pH solutions, which did not affect their dormant state, was also
very low. 相似文献
99.
Herein, we present results from molecular dynamics simulations of the DD-transpeptidase/penicillin-binding protein from Streptomyces K15 and its Michaelis complex with benzylpenicillin. For the apo-enzyme, six different configurations of the active site were modeled in aqueous solution and their relative stabilities were estimated by means of quantum mechanical energy calculations. The energetically most stable configuration has a neutral Lys(213) residue. In this configuration, the nucleophilic Ser(35) hydroxyl group interchanges with a water molecule in the "oxy-anion hole" and the Lys(38)/Lys(213) ammonium/amino groups are connected through the Ser(96) hydroxyl group. Subsequently, the enzyme-penicillin complexes corresponding to the four most stable configurations of the apo-enzyme were modeled. In the presence of the beta-lactam antibiotic, the configuration with a neutral Lys(38) residue is favored energetically and shows the best orientation for nucleophilic attack. In addition, a very stable contact between the Ser(35) hydroxyl group and the neutral amino group of Lys(38) supports the assignation of Lys(38) as the base catalyst for the acylation step. Finally, some mechanistic implications of enzyme-inhibitor contacts involving the benzylpenicillin carboxylate group are also discussed. 相似文献
100.
The effects of coating thickness, type of adhesive, and type and concentration of enhancer on the mechanical properties of two acrylic pressure-sensitive adhesives (PSAs) were investigated using a 2(4) factorial design and an optimization technique. Sixteen formulations containing 0% or 10% of either caprylic acid or methyl laurate in two different PSAs, namely Duro-Tak 87-2196 and Duro-Tak 87-2097, were prepared. The adhesive properties of these laminates were evaluated by applying the 90 degrees Dynamic Adhesive Strength Peel Test (90 degrees DASPT) and 1800 Release Liner Peel Test (180 degrees RLPT). Coating thickness, concentration of enhancer, and type of adhesive did affect the 90 degrees DASPT. For the 180 degrees RLPT, the most significant factors were coating thickness and concentration of enhancer, with a strong interaction observed between the two. Coating thickness and concentration of enhancer were also used to create mathematical models that correlated these factors with the mechanical properties of the PSAs. For this purpose, the optimization technique 3(2) was applied. It was found that the correlation of the above factors can be adequately described with polynomial equations, which can be used for predicting the mechanical properties of the laminates containing the above PSAs and methyl laurate (0%-10%). 相似文献