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51.
Parry DA Logan CV Hayward BE Shires M Landolsi H Diggle C Carr I Rittore C Touitou I Philibert L Fisher RA Fallahian M Huntriss JD Picton HM Malik S Taylor GR Johnson CA Bonthron DT Sheridan EG 《American journal of human genetics》2011,89(3):451-458
Familial biparental hydatidiform mole (FBHM) is the only known pure maternal-effect recessive inherited disorder in humans. Affected women, although developmentally normal themselves, suffer repeated pregnancy loss because of the development of the conceptus into a complete hydatidiform mole in which extraembryonic trophoblastic tissue develops but the embryo itself suffers early demise. This developmental phenotype results from a genome-wide failure to correctly specify or maintain a maternal epigenotype at imprinted loci. Most cases of FBHM result from mutations of NLRP7, but genetic heterogeneity has been demonstrated. Here, we report biallelic mutations of C6orf221 in three families with FBHM. The previously described biological properties of their respective gene families suggest that NLRP7 and C6orf221 may interact as components of an oocyte complex that is directly or indirectly required for determination of epigenetic status on the oocyte genome. 相似文献
52.
Evolution of herbicide resistance in weeds is a growing problem across the world, and it has been suggested that low herbicide rates may be contributing to this problem. An individual-based simulation model that represents weed population dynamics and the evolution of polygenic herbicide resistance was constructed and used to investigate whether using lower herbicide rates or standard rates at reduced efficacy could reduce the sustainability of cropping systems by causing faster increases in weed population density as herbicide resistance develops. A number of different possible genetic bases for resistance were considered, including monogenic resistance and polygenic resistance conferred by several genes. The results show that cutting herbicide rates does not affect the rate at which weed densities reach critical levels when resistance is conferred exclusively by a single dominant gene. In some polygenic situations, cutting herbicide rates substantially reduces sustainability, due to a combination of faster increase in resistance gene frequency and reduced kill rates in all genotypes, while in other polygenic situations the effect is small. Differences in sustainability depend on combined strength of the resistance genes, variability in phenotypic susceptibility and rate delivered, level of control due to alternative measures, and degree of genetic dominance and epistasis. In the situation where resistance can be conferred by both a single dominant major gene or a number of co-dominant minor genes in combination, the difference made by low rates depends on the relative initial frequency of the major and minor genes. These results show that careful consideration of herbicide rate and understanding the genetic basis of resistance are important aspects of weed management. 相似文献
53.
Andromonoecy is hypothesized to evolve as a mechanism enabling plants to independently allocate resources to female and male function. If staminate flower production is a mechanism to regulate allocation to female function (i.e., fruit production), then large-fruited species should be more strongly andromonoecious than smaller-fruited taxa because more resources are required to mature large fruit. We combined phylogenetically independent contrast analyses with extensive phenotypic characterization under common greenhouse conditions to examine the predicted relationship between fruit mass and the strength of andromonoecy among 13 species in Solanum sections Acanthophora and Lasiocarpa. The strength of andromonoecy, defined as the proportion of staminate flowers produced within inflorescences, was significantly and positively associated with fruit mass in both na?ve and phylogenetically independent analyses. Our results are consistent with the hypothesis that andromonoecy functions as a mechanism to regulate allocation to female function and suggest that the strength of andromonoecy is also associated with resource limitation. In general, we find that strong andromonoecy appears to arise via reductions in hermaphroditic flower number. However, increases in staminate flowers have also contributed to transitions to strong andromonoecy in certain species. Finally, our analyses identified a suite of correlated characters (flower size, ovary width, fruit mass) that are associated with changes in the sexual expression of andromonoecy. 相似文献
54.
55.
Pseudomonas aeruginosa produces the cell-to-cell signal molecule 2-heptyl-3-hydroxy-4-quinolone (The Pseudomonas quinolone signal; PQS), which is integrated within a complicated quorum sensing signaling system. PQS belongs to the family of 2-alkyl-4-quinolones (AQs), which have been previously described for their antimicrobial activities. PQS is synthesized via the pqsABCDE operon which is responsible for generating multiple AQs including 2-heptyl-4-quinolone (HHQ), the immediate PQS precursor. In addition, PQS signaling plays an important role in P. aeruginosa pathogenesis because it regulates the production of diverse virulence factors including elastase, pyocyanin and LecA lectin in addition to affecting biofilm formation. Here, we summarize the most recent findings on the biosynthesis and regulation of PQS and other AQs including the discovery of AQs in other bacterial species. 相似文献
56.
Pamela K. Diggle 《American journal of botany》1991,78(3):377-393
Sex expression (the proportions of hermaphrodite and staminate flowers produced) of the andromonoecious species Solatium hirtum is labile, and this lability of whole plant sex expression is due to labile sex expression of individual floral buds. In this paper I examine the developmental processes that underlie the differences in floral sex expression of hermaphrodite and staminate flowers of Solarium hirtum, focusing particularly on the processes responsible for the observed lability of floral sex expression. Differences in bud growth rate and relative growth of floral organs in these buds are evident at about the time of megasporocyte meiosis (11–12 days before anthesis). However, gynoecial sterility in staminate buds does not occur until just 6–7 days before anthesis. At this time, abnormalities in ovule development occur in staminate buds: the ovules begin to appear necrotic, the integumentary tapetum collapses, and the megagametophytes of many ovules cease normal development. These observations are consistent with the predictions of labile floral development. 相似文献
57.
A Sequence-Tagged Site Map of Human Chromosome 11 总被引:1,自引:0,他引:1
Michael W. Smith Stephen P. Clark Jane S. Hutchinson Yalin H. Wei Allan C. Churukian Lori B. Daniels Karin L. Diggle Michael W. Gen Anthony J. Romo Ying Lin Licia Selleri David L. McElligott Glen A. Evans 《Genomics》1993,17(3)
We report the construction of 370 sequence-tagged sites (STSs) that are detectable by PCR amplification under sets of standardized conditions and that have been regionally mapped to human chromosome 11. DNA sequences were determined by sequencing directly from cosmid templates using primers complementary to T3 and T7 promoters present in the cloning vector. Oligonucleotide PCR primers were predicted by computer and tested using a battery of genomic DNAs. Cosmids were regionally localized on chromosome 11 by using fluorescence in situ hybridization or by analyzing a somatic cell hybrid panel. Additional STSs corresponding to known genes and markers on chromosome 11 were also produced under the same series of standardized conditions. The resulting STSs provide uniform coverage of chromosome 11 with an average spacing of 340 kb. The DNA sequence determined for use in STS production corresponds to about 0.1% (116 kb) of chromosome 11 and has been analyzed for the presence of repetitive sequences, similarities to known genes and motifs, and possible exons. Computer analysis of this sequence has identified and therefore mapped at least eight new genes on chromosome 11. 相似文献
58.
Greg?Stuart-HillEmail author Richard?Diggle Bevan?Munali Jo?Tagg David?Ward 《Biodiversity and Conservation》2005,14(11):2611-2631
Namibia's Community Based Natural Resource Management program is a joint venture between government, national non-governmental
organisations and rural communities. A component of the program involves communities in monitoring various aspects of their
conservancy, ranging from wildlife numbers, through economic returns, to patrolling records and infringements of the rules.
A main feature of community monitoring is the Event Book System, which differs from conventional monitoring in that the community
dictates what needs to be monitored, and scientists only facilitate the design process and conservancy members undertake all
data analysis. The system has been adopted with good results by more than 30 communal conservancies in Namibia, covering almost
seven million ha, and is now also being piloted in six national parks. Continued emphasis is needed on enhancing community
interpretation and use of data for active adaptive management, particularly where conservancy leaders are transient due to
the democratic nature of local organizations. Moreover, because the system is driven by local priorities, it does not cover
all aspects of a comprehensive biodiversity monitoring programme. Where society deems other biodiversity values worth monitoring,
conservancies must either be willingly persuaded to act on this, or external systems must be established to cater for these
needs. If a community already has a monitoring system of its own, a win–win solution might be for the community to be sub-contracted
to undertake these ȁ8external modules' on behalf of national agencies. 相似文献
59.
Developmental preformation is ubiquitous among alpine and arctic tundra plant species and may cause a delay in plant morphological responses to environmental variation. The duration of preformation and seasonal pattern of development were examined in Caltha leptosepala to identify characteristics of architecture and development that may influence the timing of plant responses to environmental cues, both within a single growing season and between years. All structures in C. leptosepala are preformed: leaves are initiated one or two growing seasons before they mature and flowers are initiated one growing season before maturation. Features of development and architecture in C. leptosepala, however, appear to differ from the determinate growth patterns of other exclusively preforming species, and may allow within-season variability in the seasonal development and maturation of structures. Cohorts of leaves initiated are asynchronous with maturation cohorts, and each year the number of leaf primordia per plant at snowmelt exceeds the number to mature aboveground. Therefore, some flexibility in whether leaves complete a 2-yr or 3-yr developmental trajectory might occur. Plasticity in reproductive phenotype might also occur via the process of floral abortion. Despite developmental characteristics that might facilitate the expression of phenotypic plasticity, only slight variability was observed in the duration of preformation or in the seasonal pattern of initiation and emergence of structures. Growth patterns of C. leptosepala thus appear to be fundamentally constrained, and limitations to annual growth may assure that sufficient preformed primordia remain belowground at the end of each growing season for maturation of a full cohort during the subsequent season. 相似文献
60.
Gavin Melaugh Jaime Hutchison Kasper N?rskov Kragh Yasuhiko Irie Aled Roberts Thomas Bjarnsholt Stephen P. Diggle Vernita D. Gordon Rosalind J. Allen 《PloS one》2016,11(3)
Bacterial biofilms are usually assumed to originate from individual cells deposited on a surface. However, many biofilm-forming bacteria tend to aggregate in the planktonic phase so that it is possible that many natural and infectious biofilms originate wholly or partially from pre-formed cell aggregates. Here, we use agent-based computer simulations to investigate the role of pre-formed aggregates in biofilm development. Focusing on the initial shape the aggregate forms on the surface, we find that the degree of spreading of an aggregate on a surface can play an important role in determining its eventual fate during biofilm development. Specifically, initially spread aggregates perform better when competition with surrounding unaggregated bacterial cells is low, while initially rounded aggregates perform better when competition with surrounding unaggregated cells is high. These contrasting outcomes are governed by a trade-off between aggregate surface area and height. Our results provide new insight into biofilm formation and development, and reveal new factors that may be at play in the social evolution of biofilm communities. 相似文献