Antigen-binding cells in central and peripheral lymphoid tissues of the rabbit

The rosette assay was used to study antigen-binding activity by cells in lymphoid tissues of rabbits immunized with sheep red blood cells and in unimmunized controls. Percentages of rosette-forming cells (RFC) observed were compared with those of cells which secreted antibody (plaque-forming cells, PFC) and cells which both bound antigen and secreted antibody. Rosette-forming cells and PFC were shown to be two distinct reactive cell populations. Thus, in the spleen less than 1% of RFC also formed plaques. Immediately following antigen stimulation, the number of RFC in the bone marrow decreased to below detectable limits. After an initial rise, the number of RFC in the appendix declined similarly. In contrast, RFC levels in the spleen rose steadily from the time of immunization. These patterns suggest that bone marrow and appendix may function as a reservoir of antigen-binding cells which are released to other sites following antigenic stimulation. Rosette-forming cells were rarely observed in the thymus. Rosette-inhibition studies using antisera specific for bone marrow-derived cells (anti-B) and thymus-derived cells (anti-T) revealed a markedly greater proportion of T-RFC in the appendix than in the spleen.     

Expression of the argA Gene Carried by a Defective Lambda Bacteriophage of Escherichia coli

Evidence is presented that the increase in specific activity of N-acetylglutamate synthase observed upon heat induction of Escherichia coli (lambdadargA) is primarily due to a gene dosage effect.     

Der Zug des Sperbers(Accipiter nisus) auf Helgoland

Zusammenfassung Der Sperber ist auf Helgoland nur Durchzügler. Im langjährigen Mittel werden pro Jahr im Winter 0,4, auf dem Heimzug 2,1 und im Herbst 23,0 Fänglinge festgestellt. Der Zugablauf läßt eine Trennung der Zugzeiten der Alt- und Jungvögel einerseits sowie der und andererseits erkennen. Altvögel stellen 14,1%, 40,1% der Fänglinge. Anhand der Flügelmaße und Gewichte läßt sich mit fortschreitender Zugzeit keine klinale Variation nachweisen. Ältere Literaturangaben, Helgoland betreffend, werden teils korrigiert, teils präzisiert. Der geringe Anteil der Altvögel bzw. wird auf deren geringer ausgeprägten Zugtrieb zurückgeführt.

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The migration of the sparrow-hawk on Heligoland

Summary On Heligoland the Sparrow-Hawk occurs as a passage visitor only. Mean numbers (17 years) of birds trapped are 0.4 in winter, 2.1 during spring and 23.0 in autumn. Adults and juveniles as males and females as well show different periods of migration. Among birds trapped there are 14.1% adults and 40.1% females. No clinal variation of wing length and weight according to time of passage could be found. Former literature concerning Heligoland is partly corrected, partly rendered more precise. The low proportions of adults and females respectively are caused by weaker migratory urge.

     

Diosgenin saponins from Dioscorea floribunda

Five spirostanol glycosides and two furostanol glycosides were isolated from Dioscorea floribunda. In addition to the IR spectra of the free glycosides and the MS of the peracetates and permethyl ethers, the most effective method for structural determination proved to be the NMR spectra of the free saponins in pyridine-d₅.     

Ein einfaches Verfahren zur Anzucht von Farnprothallien

Ohne ZusammenfassungMit 1 AbbildungFrau Prof. Dr.E. Schiemann zum 80. Geburtstag gewidmet.     

Unprecedented Therapeutic Potential with a Combination of A2A/NR2B Receptor Antagonists as Observed in the 6-OHDA Lesioned Rat Model of Parkinson's Disease

In Parkinson''s disease, the long-term use of dopamine replacing agents is associated with the development of motor complications; therefore, there is a need for non-dopaminergic drugs. This study evaluated the potential therapeutic impact of six different NR2B and A_2A receptor antagonists given either alone or in combination in unilateral 6-OHDA-lesioned rats without (monotherapy) or with (add-on therapy) the co-administration of L-Dopa: Sch-58261+ Merck 22; Sch-58261+Co-101244; Preladenant + Merck 22; Preladenant + Radiprodil; Tozadenant + Radiprodil; Istradefylline + Co-101244. Animals given monotherapy were assessed on distance traveled and rearing, whereas those given add-on therapy were assessed on contralateral rotations. Three-way mixed ANOVA were conducted to assess the main effect of each drug separately and to determine whether any interaction between two drugs was additive or synergistic. Additional post hoc analyses were conducted to compare the effect of the combination with the effect of the drugs alone. Motor activity improved significantly and was sustained for longer when the drugs were given in combination than when administered separately at the same dose. Similarly, when tested as add-on treatment to L-Dopa, the combinations resulted in higher levels of contralateral rotation in comparison to the single drugs. Of special interest, the activity observed with some combinations could not be described by a simplistic additive effect and involved more subtle synergistic pharmacological interactions. The combined administration of A_2A/NR2B-receptor antagonists improved motor behaviour in 6-OHDA rats. Given the proven translatability of this model such a combination may be expected to be effective in improving motor symptoms in patients.