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Nader Morshed William T Ralvenius Alexi Nott L Ashley Watson Felicia H Rodriguez Leyla A Akay Brian A Joughin PingChieh Pao Jay Penney Lauren LaRocque Diego Mastroeni LiHuei Tsai Forest M White 《Molecular systems biology》2020,16(12)
Alzheimer’s disease (AD) is characterized by the appearance of amyloid‐β plaques, neurofibrillary tangles, and inflammation in brain regions involved in memory. Using mass spectrometry, we have quantified the phosphoproteome of the CK‐p25, 5XFAD, and Tau P301S mouse models of neurodegeneration. We identified a shared response involving Siglec‐F which was upregulated on a subset of reactive microglia. The human paralog Siglec‐8 was also upregulated on microglia in AD. Siglec‐F and Siglec‐8 were upregulated following microglial activation with interferon gamma (IFNγ) in BV‐2 cell line and human stem cell‐derived microglia models. Siglec‐F overexpression activates an endocytic and pyroptotic inflammatory response in BV‐2 cells, dependent on its sialic acid substrates and immunoreceptor tyrosine‐based inhibition motif (ITIM) phosphorylation sites. Related human Siglecs induced a similar response in BV‐2 cells. Collectively, our results point to an important role for mouse Siglec‐F and human Siglec‐8 in regulating microglial activation during neurodegeneration. 相似文献
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Jean Armengaud Agnès Delaunay-Moisan Jean-Yves Thuret Eelco van Anken Diego Acosta-Alvear Tomás Aragón Carolina Arias Marc Blondel Ineke Braakman Jean-François Collet René Courcol Antoine Danchin Jean-François Deleuze Jean-Philippe Lavigne Sophie Lucas Thomas Michiels Edward R. B. Moore Jonathon Nixon-Abell Ramon Rossello-Mora Zheng-Li Shi Antonio G. Siccardi Roberto Sitia Daniel Tillett Kenneth N. Timmis Michel B. Toledano Peter van der Sluijs Elisa Vicenzi 《Environmental microbiology》2020,22(6):1997-2000
The current SARS-CoV-2 pandemic is wreaking havoc throughout the world and has rapidly become a global health emergency. A central question concerning COVID-19 is why some individuals become sick and others not. Many have pointed already at variation in risk factors between individuals. However, the variable outcome of SARS-CoV-2 infections may, at least in part, be due also to differences between the viral subspecies with which individuals are infected. A more pertinent question is how we are to overcome the current pandemic. A vaccine against SARS-CoV-2 would offer significant relief, although vaccine developers have warned that design, testing and production of vaccines may take a year if not longer. Vaccines are based on a handful of different designs (i), but the earliest vaccines were based on the live, attenuated virus. As has been the case for other viruses during earlier pandemics, SARS-CoV-2 will mutate and may naturally attenuate over time (ii). What makes the current pandemic unique is that, thanks to state-of-the-art nucleic acid sequencing technologies, we can follow in detail how SARS-CoV-2 evolves while it spreads. We argue that knowledge of naturally emerging attenuated SARS-CoV-2 variants across the globe should be of key interest in our fight against the pandemic. 相似文献
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Gemma Aragonès Kalavathi Dasuri Opeoluwa Olukorede Sarah G. Francisco Carol Renneburg Caroline Kumsta Malene Hansen Shun Kageyama Masaaki Komatsu Sheldon Rowan Jonathan Volkin Michael Workman Wenxin Yang Paula Daza Diego Ruano Helena Dominguez‐Martín José Antonio Rodríguez‐Navarro Xue‐Liang Du Michael A. Brownlee Eloy Bejarano Allen Taylor 《Aging cell》2020,19(11)
36.
Monja-Mio Kelly M. Olvera-Casanova Diego Herrera-Herrera Gaston Herrera-Alamillo Miguel Ángel Sánchez-Teyer Felipe L. Robert Manuel L. 《In vitro cellular & developmental biology. Plant》2020,56(5):662-669
In Vitro Cellular & Developmental Biology - Plant - 相似文献
37.
Pedro Len‐Lobos Marcela A. Bustamante‐Snchez Cara R. Nelson Diego Alarcn Rodrigo Hasbún Michael Way Hugh W. Pritchard Juan J. Armesto 《Restoration Ecology》2020,28(2):277-281
We argue that the need for a quality seed supply chain is a major bottleneck for the restoration of Chile's native ecosystems, thus supplementing the list of bottlenecks proposed by Bannister et al. in 2018. Specifically, there is a need for defining seed transfer zones, developing standards and capacities for properly collecting and storing seeds, reducing information gaps on seed physiology and longevity, and implementing an efficient seed supply chain with certification of seed origin and quality. Without such capacities, countries are unlikely to meet their restoration commitments. Although we focus on bottlenecks in Chile, the issues we raise are relevant to other countries and thus the global agenda for ecological restoration. 相似文献
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Federico Machinandiarena Leandro Nakamatsu Gustavo E. Schujman Diego de Mendoza Daniela Albanesi 《Molecular microbiology》2020,114(4):653-663
A key aspect in membrane biogenesis is the coordination of fatty acid to phospholipid synthesis rates. In most bacteria, PlsX is the first enzyme of the phosphatidic acid synthesis pathway, the common precursor of all phospholipids. Previously, we proposed that PlsX is a key regulatory point that synchronizes the fatty acid synthase II with phospholipid synthesis in Bacillus subtilis. However, understanding the basis of such coordination mechanism remained a challenge in Gram-positive bacteria. Here, we show that the inhibition of fatty acid and phospholipid synthesis caused by PlsX depletion leads to the accumulation of long-chain acyl-ACPs, the end products of the fatty acid synthase II. Hydrolysis of the acyl-ACP pool by heterologous expression of a cytosolic thioesterase relieves the inhibition of fatty acid synthesis, indicating that acyl-ACPs are feedback inhibitors of this metabolic route. Unexpectedly, inactivation of PlsX triggers a large increase of malonyl-CoA leading to induction of the fap regulon. This finding discards the hypothesis, proposed for B. subtilis and extended to other Gram-positive bacteria, that acyl-ACPs are feedback inhibitors of the acetyl-CoA carboxylase. Finally, we propose that the continuous production of malonyl-CoA during phospholipid synthesis inhibition provides an additional mechanism for fine-tuning the coupling between phospholipid and fatty acid production in bacteria with FapR regulation. 相似文献
40.
Sáenz-de la O Diana Cedillo-Jimenez Christopher Alexis García-Ortega Luis F. Martínez-Reséndiz Mariela Arné-Robles Diego Cruz-Hernandez Andrés Guevara-Gonzalez Ramón Gerardo 《Physiology and Molecular Biology of Plants》2020,26(1):3-13
Physiology and Molecular Biology of Plants - Transgenic tobacco (N. tabacum cv. Xanthi nc) expressing Capsicum chinense CchGLP gene that encodes an Mn-SOD, constitutively produces hydrogen... 相似文献