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941.
Distinctive probiotic features share common TLR2‐dependent signalling in intestinal epithelial cells
Diana Paveljek Karolina Ivi
ak‐Kocjan Primo Treven Mojca Ben
ina Roman Jerala Irena Rogelj 《Cellular microbiology》2021,23(1)
The underlying mechanisms of probiotics and postbiotics are not well understood, but it is known that both affect the adaptive and innate immune responses. In addition, there is a growing concept that some probiotic strains have common core mechanisms that provide certain health benefits. Here, we aimed to elucidate the signalization of the probiotic bacterial strains Lactobacillus paragasseri K7, Limosilactobacillus fermentum L930BB, Bifidobacterium animalis subsp. animalis IM386 and Lactiplantibacillus plantarum WCFS1. We showed in in vitro experiments that the tested probiotics exhibit common TLR2‐ and TLR10‐dependent downstream signalling cascades involving inhibition of NF‐κB signal transduction. Under inflammatory conditions, the probiotics activated phosphatidylinositol 3‐kinase (PI3K)/Akt anti‐apoptotic pathways and protein kinase C (PKC)‐dependent pathways, which led to regulation of the actin cytoskeleton and tight junctions. These pathways contribute to the regeneration of the intestinal epithelium and modulation of the mucosal immune system, which, together with the inhibition of canonical TLR signalling, promote general immune tolerance. With this study we identified shared probiotic mechanisms and were the first to pinpoint the role of anti‐inflammatory probiotic signalling through TLR10. 相似文献
942.
943.
Qi Shaohua Ngwa Conelius Morales Scheihing Diego A. Al Mamun Abdullah Ahnstedt Hilda W. Finger Carson E. Colpo Gabriela Delevati Sharmeen Romana Kim Youngran Choi HuiMahn A. McCullough Louise D. Liu Fudong 《Biology of sex differences》2021,12(1):1-11
Determine if sex differences exist in clinical characteristics and outcomes of adults hospitalized for coronavirus disease 2019 (COVID-19) in a US healthcare system. Case series study. Sequentially hospitalized adults admitted for COVID-19 at two tertiary care academic hospitals in New Orleans, LA, between 27 February and 15 July 2020. Measures included demographics, comorbidities, presenting symptoms, and laboratory results. Outcomes included intensive care unit admission (ICU), invasive mechanical ventilation (IMV), and in-hospital death. We included 776 patients (median age 60.5 years; 61.4% women, 75% non-Hispanic Black). Rates of ICU, IMV, and death were similar in both sexes. In women versus men, obesity (63.8 vs 41.6%, P < 0.0001), hypertension (77.6 vs 70.1%, P = 0.02), diabetes (38.2 vs 31.8%, P = 0.06), chronic obstructive pulmonary disease (COPD, 22.1 vs 15.1%, P = 0.015), and asthma (14.3 vs 6.9%, P = 0.001) were more prevalent. More women exhibited dyspnea (61.2 vs 53.7%, P = 0.04), fatigue (35.7 vs 28.5%, P = 0.03), and digestive symptoms (39.3 vs 32.8%, P = 0.06) than men. Obesity was associated with IMV at a lower BMI (> 35) in women, but the magnitude of the effect of morbid obesity (BMI ≥ 40) was similar in both sexes. COPD was associated with ICU (adjusted OR (aOR), 2.6; 95%CI, 1.5–4.3) and IMV (aOR, 1.8; 95%CI, 1.2–3.1) in women only. Diabetes (aOR, 2.6; 95%CI, 1.2–2.9), chronic kidney disease (aOR, 2.2; 95%CI, 1.3–5.2), elevated neutrophil-to-lymphocyte ratio (aOR, 2.5; 95%CI, 1.4–4.3), and elevated ferritin (aOR, 3.6; 95%CI, 1.7–7.3) were independent predictors of death in women only. In contrast, elevated D-dimer was an independent predictor of ICU (aOR, 7.3; 95%CI, 2.7–19.5), IMV (aOR, 6.5; 95%CI, 2.1–20.4), and death (aOR, 4.5; 95%CI, 1.2–16.4) in men only. This study highlights sex disparities in clinical determinants of severe outcomes in COVID-19 patients that may inform management and prevention strategies to ensure gender equity. 相似文献
944.
Jean‐Pierre Desforges Floris M. van Beest Gonalo M. Marques Stine H. Pedersen Larissa T. Beumer Marianna Chimienti Niels Martin Schmidt 《Ecology and evolution》2021,11(1):338-351
Animals have adapted behavioral and physiological strategies to conserve energy during periods of adverse conditions. Heterothermy is one such adaptation used by endotherms. While heterothermy—fluctuations in body temperature and metabolic rate—has been shown in large vertebrates, little is known of the costs and benefits of this strategy, both in terms of energy and in terms of fitness. Hence, our objective was to model the energetics of seasonal heterothermy in the largest Arctic ungulate, the muskox (Ovibos moschatus), using an individual‐based energy budget model of metabolic physiology. We found that the empirically based drop in body temperature (winter max ~−0.8°C) overwinter in adult females resulted in substantial fitness benefits in terms of reduced daily energy expenditure and body mass loss. Body mass and energy reserves were 8.98% and 14.46% greater in modeled heterotherms compared to normotherms by end of winter. Based on environmental simulations, we show that seasonal heterothermy can, to some extent, buffer the negative consequences of poor prewinter body condition or reduced winter food accessibility, leading to greater winter survival (+20%–30%) and spring energy reserves (+10%–30%), and thus increased probability of future reproductive success. These results indicate substantial adaptive short‐term benefits of seasonal heterothermy at the individual level, with potential implications for long‐term population dynamics in highly seasonal environments. 相似文献
945.
Sunghyun Woo Dongyeob Lee Yong‐Chan Cho Sangsun Lee Eunsuk Kim 《Ecology and evolution》2021,11(1):516-525
Comparative studies of invasive, noninvasive alien, and native congenic plant species can identify plant traits that drive invasiveness. In particular, functional traits associated with rapid growth rate and high fecundity likely facilitate invasive success. As such traits often exhibit high phenotypic plasticity, characterizing plastic responses to anthropogenic environmental changes such as eutrophication and disturbance is important for predicting the invasive success of alien plant species in the future. Here, we compared trait expression and phenotypic plasticity at the species level among invasive, noninvasive alien, and native Bidens species. Plants were grown under nutrient addition and competition treatments, and their functional, morphological, and seed traits were examined. Invasive B. frondosa exhibited higher phenotypic plasticity in most measured traits than did the alien noninvasive B. pilosa or native B. bipinnata. However, differential plastic responses to environmental treatments rarely altered the rank of trait values among the three Bidens species, except for the number of inflorescences. The achene size of B. frondosa was larger, but its pappus length was shorter than that of B. pilosa. Two species demonstrated opposite plastic responses of pappus length to fertilization. These results suggest that the plasticity of functional traits does not significantly contribute to the invasive success of B. frondosa. The dispersal efficiency of B. frondosa is expected to be lower than that of B. pilosa, suggesting that long‐distance dispersal is likely not a critical factor in determining invasive success. 相似文献
946.
Zoraida Calle Natalia Henao‐Gallego Carolina Giraldo Inge Armbrecht 《Restoration Ecology》2013,21(6):729-735
Landslides and gullies are two common manifestations of land degradation in the densely populated Colombian Andes. In these unstable areas, further mass movements pose a serious threat to local populations and cause off‐site environmental damage through sedimentation, pollution, and increased flooding. A novel approach for restoring severely eroded slopes combines the use of stabilization structures made with stalks of Guadua angustifolia Kunth, Poaceae (bamboo), with high‐density planting of species that exhibit quick growth and sprouting. This study compared the vegetation and ground‐dwelling ant assemblages of 10 pairs of gullies, each pair formed by one enhanced and one untreated or control gully, 6–8 years after restoration or abandonment. The restoration treatment had significant effects on the complexity of vegetation. Average values for plant species richness, basal area, stem density, foliage density index, and total vegetation volume were 11.6, 140, 30, 11.5, and 15.6 times larger, respectively, in enhanced than in control gullies. Mirroring the differences in vegetation, average ant species richness was significantly larger (13 vs. 7.6 species per gully), and a higher proportion of ant species nested within enhanced than control gullies (52.5 vs. 30%). While control gullies were dominated by generalist ants such as Ectatomma ruidum and Linepithema angulatum, enhanced gullies had more specialized ground‐dwelling species, normally associated with high plant cover and abundant leaf litter such as Octostruma balzani and Heteroponera inca. We conclude that this restoration strategy promotes a fast recovery of vegetation and the ground‐dwelling ant fauna in these tropical mountains. 相似文献
947.
For marine fish and invertebrates, larval dispersal plays a critical role in determining connections among source and sink habitats, and the lack of a predictive understanding of larval dispersal is a fundamental obstacle to the development of spatially explicit restoration plans for marine populations. We investigated larval dispersal patterns of eastern oyster in an estuary along the Northern Gulf of Mexico under different simulation scenarios of tidal amplitude and phase, river discharge, wind direction, and larval vertical migration, using a coupled biophysical transport model. We focused on the dispersal of larvae released from the commercially exploited (Cedar Point, CP) and non‐exploited (Bon Secour Bay, BSB) oyster populations. We found that high flushing rates through the dominant inlet prevented larval exchange between the commercially exploited and non‐exploited populations, resulting in negligible connectivity between them. Variations in tidal amplitude, river discharge and wind direction played a more important role in the amount of larvae retained in Mobile Bay when they are released from CP than from BSB. Under most of the scenarios, larvae from BSB were retained around the spawning area, while larvae from CP showed a predominant westward flow. Net sinking behavior of late‐stage larvae increased larval retention in the bay, but physical transport showed a higher impact in the amount of larvae retained. These findings have enhanced our understanding of larval dispersal of eastern oyster in a wide, shallow estuarine system, and been used to establish spatially explicit strategies for oyster restoration in the Mobile Bay system, Alabama. 相似文献
948.
Disturbed natural areas frequently experience invasion by introduced plant species that can reduce native biodiversity. Biological control can suppress these introduced species, but without restoration another introduced species can invade. Integration of biological control with concurrent revegetation can both aid in weed reduction via interspecific plant competition and establish a restored native plant community. This 3‐year study investigated an integrated approach to controlling the introduced annual Mile‐a‐minute weed (Persicaria perfoliata [L.] H. Gross [Polygonaceae]) using the biocontrol weevil Rhinoncomimus latipes Korotyaev (Coleoptera: Curculionidae) and restoration planting using a native seed mix. A fully factorial design tested weevils and seeding, separately and together, using insecticide to eliminate weevils. The weevils together with the native seed mix reduced P. perfoliata percent cover in 2009 and 2010, and peak seed cluster production in 2010, compared to the insecticide ? no seed control treatment. Persicaria perfoliata final dry biomass was reduced by 75% in 2010 and by 57% in 2011 in the weevils plus seed treatment compared to the control, with weevils having the greatest effect in 2010 and the seed treatment having the greatest impact in 2011. Results suggest an additive effect of biocontrol and seeding in suppressing P. perfoliata. Seeded treatments also developed the highest native plant species richness and diversity, comprised of spontaneous recolonization in addition to species from the seed mix. Results support the use of integrated management of this invasive weed, with suppression through biological control and native revegetation together helping prevent reinvasion while restoring native plant biodiversity. 相似文献
949.
In revegetation projects, distinguishing species that can be passively restored by natural regeneration from those requiring active restoration is not a trivial decision. We quantified tree species dominance (measured by an importance value index, IVIi) and used abundance–size correlations to select those species suitable for passive and/or active restoration of disturbed riparian vegetation in the Lacandonia region, Southern Mexico. We sampled riparian vegetation in a 50 × 10–m transect in each of six reference (RE) and five disturbed (DE) riparian ecosystems. Those species representing more than 50% of total IVI in each ecosystem were selected, and Spearman rank correlation between abundance and diameter classes was calculated. For eight species, it was determined that passive restoration could be sufficient for their establishment. Another eight species could be transplanted by means of active restoration. Five species regenerate well in only one ecosystem type, suggesting that both restoration strategies could be used depending on the degree of degradation. Finally, two species were determined to not be suitable for restoration in the RE (based on the above selection criteria) and were not selected during this initial stage of our restoration project. The high number of tree species found in the RE suggests that the species pool for ecological restoration is large. However, sampling in both ecosystem types helped us reduce the number of species that requires active restoration. Restoration objectives must guide the selection of which methods to implement; in different conditions, other criteria such as dispersal syndrome or social value could be considered in the species selection. 相似文献
950.
The consequences of inbreeding on fitness can be crucial in evolutionary and conservation grounds and depend upon the efficiency of purging against deleterious recessive alleles. Recently, analytical expressions have been derived to predict the evolution of mean fitness, taking into account both inbreeding and purging, which depend on an ‘effective purging coefficient (de)’. Here, we explore the validity of that predictive approach and assay the strength of purging by estimating de for egg‐to‐pupae viability (EPV) after a drastic reduction in population size in a recently captured base population of Drosophila melanogaster. For this purpose, we first obtained estimates of the inbreeding depression rate (δ) for EPV in the base population, and we found that about 40% was due to segregating recessive lethals. Then, two sets of lines were founded from this base population and were maintained with different effective size throughout the rest of the experiment (N = 6; N = 12), their mean EPV being assayed at different generations. Due to purging, the reductions in mean EPV experienced by these lines were considerably smaller than the corresponding neutral predictions. For the 60% of δ attributable to nonlethal deleterious alleles, our results suggest an effective purging coefficient de > 0.02. Similarly, we obtain that de > 0.09 is required to roughly account for purging against the pooled inbreeding depression from lethal and nonlethal deleterious alleles. This implies that purging should be efficient for population sizes of the order of a few tens and larger, but might be inefficient against nonlethal deleterious alleles in smaller populations. 相似文献