2-吗啉乙磺酸(MES)对拟南芥幼苗生长的影响

本实验研究0.05% MES对野生型拟南芥生长的影响。结果表明,含有MES的培养基pH变化较小,其培养10 d的拟南芥幼苗干重、鲜重、叶绿素含量和含水量均高于对照组,而叶片PAL和POD活性却低于对照组,说明MES通过影响培养基的pH变化促进拟南芥生长。     

实时荧光PCR结合融解曲线分析快速鉴定临床常见曲霉菌

目的 采用实时荧光PCR结合融解曲线分析的方法快速将临床常见曲霉菌鉴定到种的水平.方法 ①普通PCR扩增真菌ITS区后进行序列比对,准确鉴定菌种并设计种特异性引物和探针.②采用实时荧光PCR的方法及融解曲线分析,根据不同的解链温度将5种临床常见曲霉菌鉴定到种的水平.③特异性、敏感性、重复性试验.结果 ①解链曲线分析显示,不同种曲霉菌的种特异性探针有特异的Tm值,根据Tm值的不同可以将5种曲霉菌区分开来:烟曲霉Tm =61.4℃,黄曲霉Tm=57.4℃,黑曲霉Tm=67.7℃,土曲霉Tm=55.2℃和64.5℃,构巢曲霉Tm=65.8℃.其中小孢根霉和疣状瓶霉与烟曲霉探针发生交叉反应,阴性对照不出现特异性的解链曲线.②该方法对5种曲霉菌的检测下限分别为:烟曲霉56.8 fg,黄曲霉1 110fg,黑曲霉13.7 fg,土曲霉123 fg,构巢曲霉780 fg.③重复性试验结果显示,同一种曲霉菌的Tm值波动范围不超过0.5℃.结论 采用实时荧光PCR结合融解曲线分析的方法可以快速准确地将临床常见曲霉菌鉴定到种的水平,具有良好的敏感性、特异性和可重复性,有助于临床侵袭性曲霉感染的诊断和指导抗真菌药物使用.     

鸡减蛋综合征病毒（EDSV—76）末端前体蛋白的基因结构分析

从中国发病鸡群中分离的鸡减蛋综合征病毒弱毒株ＡＡ－２,经常规方法提取其病毒核酸后,组建了完整的限制性内切酶ＰｓｔＩ及ＨｉｎｇⅢ水解片段的基因文库,并对其中ＨｉｎｄⅢ,－ＳａｃⅠ进行了序列测定。同源比较分析证明：其Ｌ链含编码病毒末端前体蛋白,容量为５８０个氨基酸残基的开放读码框架。     

Overexpression of Jatropha curcas Defensin (JcDef) Enhances Sheath Blight Disease Resistance in Tobacco

Plant defensins are small, basic, cysteine‐rich peptides, belonging to the antimicrobial peptide superfamily, commonly found in the plant kingdom. In this study, we cloned and characterized a plant defensin gene from Jatropha curcas (JcDef). JcDef carried conserved receptor binding sites and a cysteine motif, and it was phylogenetically grouped together with defensin Ec‐AMP‐D2‐like in Elaeis guineensis. JcDef is localized to cytoplasm and highly expressed in young tissues with fast metabolism such as cotyledons and stem apexes. Transgenic expression of JcDef in tobacco showed enhanced resistance against sheath blight disease caused by R. solani, indicating the antibacterial function.     

Toxoplasma gondii: Protective effect of an intranasal SAG1 and MIC4 DNA vaccine in mice

Infections by the intracellular protozoan parasite Toxoplasma gondii are widely prevalent in humans and other animals which can cause severe or lethal toxoplasmosis. So the development of a more effective vaccine is needed urgently. A multiantigenic vaccine against toxoplasmosis was constructed in the present study, which contains two T. gondii antigens, SAG1 and MIC4 on the basis of previous immunological and immunization studies. The eukaryotic plasmid pcDNA3.1-SAG1-MIC4, pcDNA3.1-SAG1, pcDNA3.1-MIC4 were constructed first, which can express surface protein SAG1 and microneme protein MIC4 from different stages of T. gondii life cycle, and the expression ability of these DNA vaccine in HeLa cells were examined by Western blot. The efficacy of these plasmids with or without co-administration of a plasmid encoding cholera toxin A2/B as a genetic adjuvant by mucosal way to protect BALB/c mice against toxoplasmosis was evaluated. We found these vaccines were able to elicit a significant humoral and cellular immune response in vaccinated mice and they can increase survival rate and prolong the life of mice that were infected by T. gondii especially in the pcDNA3.1-SAG1-MIC4 group. Co-delivery of cholera toxin A2/B further enhanced the potency of multiantigenic DNA vaccine by intranasal route. These results encourage further research towards achieving vaccinal protection against the T. gondii in animals and humans.     

Endometrial stem cell transplantation restores dopamine production in a Parkinson’s disease model

Parkinson’s disease (PD) is a neurodegenerative disorder caused by the loss of dopaminergic neurons. Adult human endometrial derived stem cells (HEDSC), a readily obtainable type of mesenchymal stem‐like cell, were used to generate dopaminergic cells and for transplantation. Cells expressing CD90, platelet derived growth factor (PDGF)‐Rβ and CD146 but not CD45 or CD31 were differentiated in vitro into dopaminergic neurons that exhibited axon projections, pyramidal cell bodies and dendritic projections that recapitulate synapse formation; these cells also expressed the neural marker nestin and tyrosine hydroxylase, the rate‐limiting enzyme in dopamine synthesis. Whole cell patch clamp recording identified G‐protein coupled inwardly rectifying potassium current 2 channels characteristic of central neurons. A 1‐methyl 4‐phenyl 1,2,3,6‐tetrahydro pyridine induced animal model of PD was used to demonstrate the ability of labelled HEDSC to engraft, migrate to the site of lesion, differentiate in vivo and significantly increase striatal dopamine and dopamine metabolite concentrations. HEDSC are a highly inducible source of allogenic stem cells that rescue dopamine concentrations in an immunocompetent PD mouse model.     

The DLC-1 -29A/T polymorphism is not associated with nasopharyngeal carcinoma risk in Chinese population

Deleted in liver cancer-1 (DLC-1), encoding a Rho GTPase-activating protein (GAP), is considered as a promising candidate tumor suppressor gene in nasopharyngeal carcinoma (NPC). The single-nucleotide polymorphism (SNP) -29A/T upstream of ATG start codon was found when gene mutation profile of DLC-1 in NPC was analyzed. To evaluate the correlation between SNP -29A/T in the promoter region of DLC-1 gene and risk of NPC, a total of 521 samples from a Chinese population, including 320 healthy individuals and 201 NPC patients, were collected for SNP analysis by PCR-single-strand conformation polymorphism and sequencing. The differences in allele and genotype frequencies between NPC patients and controls were tested using logistic regression statistical method. No significant differences were found in allele or genotype frequencies between NPC patients and controls or among different NPC clinical stages. Hence, our data indicate that the SNP -29A/T of DLC-1 gene is not associated with NPC susceptibility.     

HIFU治疗下生物体焦域温度分布的研究与应用

高强度聚焦超声(HIFU)为无创治疗肿瘤带来了新的途径,本文对HIFU治疗下生物体焦域热分布和热传导模型的理论进行了初步的介绍,并在理论的指导下进行不同治疗剂量的实验研究,结果表明组织内具有热波传热效应,治疗时可以根据治疗剂量的需要选取不同的参数,以达到最佳治疗效果。     

家蚕线粒体ND2、COⅠ和若干tRNA基因的克隆及序列分析

克隆并测定了家蚕（Bombyx mori）线粒体基因组3468bp的EcoRⅠ和HindⅢ双酶一段序列,根据序列同源性比较,该DNA片段包括3个蛋白质编码基因：ND2基因、COⅠ基因和COⅡ基因5′端399bp的序列,以及6个tRNA基因和一个尚待确定的tRNA^Met基因。家蚕与果蝇的ND2基因序列同源性约69.7%,COⅠ基因的同源性约83.8%,COⅡ基因5′端的同源性约80％,这表明细胞色素氧化酶基因在物种间比烟酰胺腺漂呤二核苷酸脱氢酶基因保守,6个推定的tRNA基因序列与果蝇相应tRNA基因序列差异较大,另外,除tRNA^Chn基因的二级结构相似外,其它tRNA基因的二级结构与果蝇相应tRNA基因的二级结构也有较大差异。