Folate metabolism in the superior cervical ganglion histochemical study.

By means of histochemical methods, folic acid, dihydrofolate reductase and NADH2-cytochrome-C-reductase were studied in the bovine superior cervical ganglion, in parallel with quantitative estimations of dihydrofolate reductase activity and in connection with the process of ageing. Various levels of folate metabolism were present in nerve cells and glial cells, as well as in pre or postganglionic nerves. In the process of ageing the activity of dihydrofolate reductase gradually decreased and the folic acid concentration in the nerve cells increased. Thus the enzyme --- substrate ratio appeared to favour the enzyme in young animals but the substrate in old animals.     

Histochemistry of human cardiac muscle in ontogenesis. Variations in the dehydrogenase and phosphatase systems

Synopsis By means of histoenzymological methods, the development of some oxido-reductase and phosphatase systems was studied in the myocardium of man during ontogenesis. Three metabolic embryonal stages with distinct enzymatic and functional characteristics were found and are described.