Investigation of lipid-exchange lipoproteins from rat liver and hepatoma 27]

A phospholipid exchange lipoprotein from the postmicrosomal supernatant of rat hepatoma 27, which stimulated in vitro the exchange of sphingomyelin between mitochondria and microsomes, was found. Sphingomyelin is incorporated into the mitochondria under incubation of this complex with rat liver mitochondria (in which sphingomyelin is absent) an microsomes. Under the same conditions the phospholipid exchange lipoproteins of rat liver do not transfer sphingomyelin form microsomes to mitochrondria.     

Glycosphingolipids from human T-lymphoma MOLT-4 cells

The glycosphingolipids of human lymphoma MOLT-4 cells were studied, using biochemical methods and specific antisera to gangliosides. The major neutral glycosphingolipids were found to be glucosyl- and lactosyl ceramides. GM3, GM2, GM1 and GD1a were identified as ganglioside components.     

Biologically effective sphingolipids devoid of 4-trans-double bond in the sphingoid hydrocarbon chain

The bioregulatory functions of sphingolipids devoid of 4E-double bond in the sphingoid chain are discussed; the sphingolipids are shown to be biologically active. The English version of the paper.     

Phospholipid composition of mitochondria and microsomes from some transplantable rat tumors

The phospholipid compositions of mitochondria and microsomes from rat liver, kidney, nephroma RA and sarcoma 45 were investigated. The "lipid dedifferentiation" of hepatoma membranes found earlier was shown to extend also to other tumours. However, this phenomenon may concern some, but not all phospholipids.     

Gangliosides from human placenta

Gangliosides of human placenta were studied, using biochemical methods and specific antibodies. The placenta was found to contain three types of gangliosides with oligosaccharide chains Lac, GgOse4 and nLcOse4.     

Comparative study of gangliosides from murine B- and T-lymphomas

Gangliosides from murine B-lymphomas (MOPC 21 and MOPC 406) and T-lymphoma EL-4 were studied by thin-layer chromatography, immunoprecipitation with specific antisera to gangliosides and by treatment with neuraminidase. It was found that the gangliosides of all three lymphomas differ in their interaction with antisera and neuraminidase although they are similar in their chromatographic behaviour.